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The pathophysiological mechanisms of aggregate formation and the role of A\u03b2 proteoforms need thorough study both to understand the role played by specific processes in the initiation of neuronal degradation and to find effective preventive means of therapeutic action. The present work investigates the dynamics of accumulation of phosphorylated serine-8 proteoform A\u03b2 (pSer8-A\u03b2) using the 5xFAD mouse amyloid model. A\u03b2 samples from human cerebrospinal fluid (CSF) and brain were also investigated. Western blot studies using 1E4E11 and 4G8 antibodies showed that accumulation of pSer8-A\u03b2 in mouse brain starts as early as at the age of 3 months and reaches a maximum by the age of 14\u201317 months, which is generally similar to the dynamics of accumulation of the total pool of A\u03b2 peptides. The pSer8-A\u03b2 level in human CSF in AD patients can reach ~ 1\u201310% of the total amount of A\u03b2. Mass spectrometric analysis showed that A\u03b2 phosphorylation by the Ser8, Tyr10, and Ser26 residues in brain tissues, as well as phosphorylation of the APP by Thr719 residue, is possible. These findings support the assumption that pSer8-A\u03b2 proteoforms are involved in amyloidosis in AD.<\/jats:p>","DOI":"10.32607\/actanaturae.27456","type":"journal-article","created":{"date-parts":[[2024,11,12]],"date-time":"2024-11-12T16:25:58Z","timestamp":1731428758000},"page":"93-101","source":"Crossref","is-referenced-by-count":1,"title":["The Features of Beta-Amyloid Phosphorylation in Alzheimer\u2019s Disease"],"prefix":"10.32607","volume":"16","author":[{"given":"P A.","family":"Strelnikova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. E.","family":"Bugrova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. 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