{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T08:02:10Z","timestamp":1777276930924,"version":"3.51.4"},"reference-count":12,"publisher":"Acta Naturae Ltd","issue":"1","license":[{"start":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T00:00:00Z","timestamp":1776816000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Naturae"],"abstract":"<jats:p>R-loops that contain a DNA:RNA hybrid and unpaired single-stranded DNA are important determinants of normal cell physiology and of the pathogenesis of numerous diseases. Although several different approaches to R-loop mapping in the genome have been developed, these techniques can produce conflicting results. In order to assess their robustness, a recent study by Chedin et al. compared the R-loop genomic distribution assessed using different methods in normal and cancer cell lines. Importantly, that study assumed a high degree of similarity between R-loop genomic distributions across different cellular types. Here, we compared DRIP datasets produced using the same protocol in different cell lines to show that only 26% of R-loop peaks are shared between chronic myeloid leukemia-derived HAP1 cells and human pluripotent stem cells. Meanwhile, HAP1-derived double knockout cell lines are characterized by much higher fractions of R-loop peaks that are identical both to each other (most of them) and to the R-loop peaks of their parental line (71 and 55%). We conclude that cellular type represents a major determinant of R-loop genomic distribution and, therefore, that only a systematic comparison of a large array of various cell\/tissue type-derived R-loop datasets may address the inconsistencies between different R-loop mapping techniques.<\/jats:p>","DOI":"10.32607\/actanaturae.27833","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T23:18:40Z","timestamp":1776813520000},"page":"79-82","source":"Crossref","is-referenced-by-count":0,"title":["Cellular type is a major determinant of R-loop genomic distribution"],"prefix":"10.32607","volume":"18","author":[{"given":"K. Yu.","family":"Oleynikova","sequence":"additional","affiliation":[{"name":"Research Center of Biotechnology, Russian Academy of Sciences"},{"name":"ITMO University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. A.","family":"Zhigalova","sequence":"additional","affiliation":[{"name":"Research Center of Biotechnology, Russian Academy of Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. P.","family":"Hutchins","sequence":"additional","affiliation":[{"name":"Southern University of Science and Technology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. S.","family":"Ruzov","sequence":"additional","affiliation":[{"name":"Research Center of Biotechnology, Russian Academy of Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"17808","published-online":{"date-parts":[[2026,4,22]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2025.102325"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-024-10025-6"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(86)90040-2"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-2477-7_28"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-2477-7_23"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2020.02.014"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-2477-7_7"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2020106394"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-019-0549-x"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-021-00867-2"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaf109"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1126\/science.1178955"}],"container-title":["Acta Naturae"],"original-title":[],"link":[{"URL":"https:\/\/actanaturae.ru\/2075-8251\/article\/viewFile\/27833\/1828","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/actanaturae.ru\/2075-8251\/article\/viewFile\/27833\/1828","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T07:23:37Z","timestamp":1777274617000},"score":1,"resource":{"primary":{"URL":"https:\/\/actanaturae.ru\/2075-8251\/article\/view\/27833"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,22]]},"references-count":12,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,4,22]]}},"URL":"https:\/\/doi.org\/10.32607\/actanaturae.27833","relation":{},"ISSN":["2075-8243"],"issn-type":[{"value":"2075-8243","type":"print"}],"subject":[],"published":{"date-parts":[[2026,4,22]]}}}