{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T22:16:17Z","timestamp":1777760177623,"version":"3.51.4"},"reference-count":29,"publisher":"Polish Society of Microbiologists","issue":"1","license":[{"start":{"date-parts":[[2022,2,27]],"date-time":"2022-02-27T00:00:00Z","timestamp":1645920000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Wautersiella falsenii<\/jats:italic>\n                    is a rarely non-fermenting Gram-negative bacterium and belongs to the\n                    <jats:italic>Flavobacteriaceae<\/jats:italic>\n                    family. This nosocomial pathogen can cause several human infections, especially among immunocompromised patients. Here, we describe the whole genome sequence of a clinical\n                    <jats:italic>W. falsenii<\/jats:italic>\n                    strain isolated from a urine sample of a 35-year-old woman with a urinary tract infection in Tunisia. We investigated its phenotype and genotype.\n                  <\/jats:p>\n                  <jats:p>\n                    After bacterial identification by the MALDI-TOF method, the whole-genome sequencing of this strain was performed. This isolate was not susceptible to various antibiotics, including \u03b2-lactams, aminoglycosides, and quinolones. However, it remains susceptible to imipenem (MIC = 0.25 mg\/l), ertapenem (MIC = 0.75 mg\/l), and meropenem (MIC = 0.19 mg\/l). Interestingly, the E-TEST\n                    <jats:sup>\u00ae<\/jats:sup>\n                    (MP\/MPI) showed a reduced MIC of meropenem +\/\u2212 EDTA (0.064 \u03bcg\/ml). Besides, the color change from yellow to red in the \u03b2 CARBA test only after 24 hours of incubation can be interpreted in two ways. On the one hand, as a likely low expression of the gene encoding metallo-\u03b2-lactamase. On the other hand, and more likely, it may be a false-positive result because, according to the test manufacturer's recommendations, the test should be read after 30 minutes. Perhaps, therefore, this gene is not expressed in the tested strain. Moreover, the whole-genome sequence analysis demonstrated the presence of a novel chromosomally located subclass B1 metallo-\u03b2-lactamase EBR-like enzyme, sharing 94.92% amino acid identity with a previously described carbapenemase produced by\n                    <jats:italic>Empedobacter brevis<\/jats:italic>\n                    , EBR-1. The results also showed the detection of other antibiotic resistance genes and the absence of plasmids. So far, this study is the first report on the detection of\n                    <jats:italic>W. falsenii<\/jats:italic>\n                    in Tunisia. These findings prove that\n                    <jats:italic>W. falsenii<\/jats:italic>\n                    could be a potential reservoir of antibiotic resistance genes, e.g., \u03b2-lactamases. Collaborative efforts and effective hygiene measures should be established to prevent the emergence of this species in our health care settings.\n                  <\/jats:p>","DOI":"10.33073\/pjm-2022-010","type":"journal-article","created":{"date-parts":[[2022,2,27]],"date-time":"2022-02-27T03:35:37Z","timestamp":1645932937000},"page":"73-81","source":"Crossref","is-referenced-by-count":6,"title":["Detection by Whole-Genome Sequencing of a Novel Metallo-\u03b2-Lactamase Produced by\n                    <i>Wautersiella falsenii<\/i>\n                    Causing Urinary Tract Infection in Tunisia"],"prefix":"10.33073","volume":"71","author":[{"given":"Raouaa","family":"Maaroufi","sequence":"first","affiliation":[{"name":"Aix-Marseille Universit\u00e9 MEPHI, AP-HM, IRD, IHU M\u00e9diterran\u00e9e Infection , Marseille , France"},{"name":"Laboratoire des Microorganismes et Biomol\u00e9cules Actives , Facult\u00e9 des Sciences de Tunis , Universit\u00e9 Tunis El-Manar , Tunis , Tunisie"},{"name":"Laboratoire de Recherche Sciences et Technologies de l\u2019Environnement , Institut Sup\u00e9rieur des Sciences et Technologies de l\u2019Environnement de Borj-Cedria , Hammam-Lif Universit\u00e9 de Carthage , Tunisie"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olfa","family":"Dziri","sequence":"additional","affiliation":[{"name":"Laboratoire des Microorganismes et Biomol\u00e9cules Actives , Facult\u00e9 des Sciences de Tunis , Universit\u00e9 Tunis El-Manar , Tunis , Tunisie"},{"name":"Laboratoire de Recherche Sciences et Technologies de l\u2019Environnement , Institut Sup\u00e9rieur des Sciences et Technologies de l\u2019Environnement de Borj-Cedria , Hammam-Lif Universit\u00e9 de Carthage , Tunisie"},{"name":"Unit\u00e9 de Service en Commun pour la Recherche \u00ab Plateforme G\u00e9nomique \u00bb, Institut Sup\u00e9rieur des Sciences et Technologies de l\u2019Environnement de Borj-Cedria, Hammam-Lif , Universit\u00e9 de Carthage , Tunisie"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda","family":"Hadjadj","sequence":"additional","affiliation":[{"name":"Aix-Marseille Universit\u00e9 MEPHI, AP-HM, IRD, IHU M\u00e9diterran\u00e9e Infection , Marseille , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seydina M.","family":"Diene","sequence":"additional","affiliation":[{"name":"Aix-Marseille Universit\u00e9 MEPHI, AP-HM, IRD, IHU M\u00e9diterran\u00e9e Infection , Marseille , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Marc","family":"Rolain","sequence":"additional","affiliation":[{"name":"Aix-Marseille Universit\u00e9 MEPHI, AP-HM, IRD, IHU M\u00e9diterran\u00e9e Infection , Marseille , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8294-8289","authenticated-orcid":false,"given":"Chedly","family":"Chouchani","sequence":"additional","affiliation":[{"name":"Laboratoire des Microorganismes et Biomol\u00e9cules Actives , Facult\u00e9 des Sciences de Tunis , Universit\u00e9 Tunis El-Manar , Tunis , Tunisie"},{"name":"Laboratoire de Recherche Sciences et Technologies de l\u2019Environnement , Institut Sup\u00e9rieur des Sciences et Technologies de l\u2019Environnement de Borj-Cedria , Hammam-Lif Universit\u00e9 de Carthage , Tunisie"},{"name":"Unit\u00e9 de Service en Commun pour la Recherche \u00ab Plateforme G\u00e9nomique \u00bb, Institut Sup\u00e9rieur des Sciences et Technologies de l\u2019Environnement de Borj-Cedria, Hammam-Lif , Universit\u00e9 de Carthage , Tunisie"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"18083","published-online":{"date-parts":[[2022,2,27]]},"reference":[{"key":"2026042902430251688_j_pjm-2022-010_ref_001","doi-asserted-by":"crossref","unstructured":"Bellais S, Aubert D, Naas T, Nordmann P. 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Biochem J. 1998 May 15;332 (1):145\u2013152. https:\/\/doi.org\/10.1042\/bj3320145","DOI":"10.1042\/bj3320145"},{"key":"2026042902430251688_j_pjm-2022-010_ref_021","doi-asserted-by":"crossref","unstructured":"Sato K, Fujii T, Okamoto R, Inoue M, Mitsuhashi S. Biochemical properties of beta-lactamase produced by Flavobacterium odoratum. Antimicrob Agents Chemother. 1985 Apr;27(4):612\u2013614. https:\/\/doi.org\/10.1128\/AAC.27.4.612","DOI":"10.1128\/AAC.27.4.612"},{"key":"2026042902430251688_j_pjm-2022-010_ref_022","doi-asserted-by":"crossref","unstructured":"Smith CJ, Owen C, Kirby L. Activation of a cryptic streptomycin-resistance gene in the Bacteroides erm transposon, Tn4551. Mol Microbiol. 1992 Aug;6(16):2287\u20132297. https:\/\/doi.org\/10.1111\/j.1365-2958.1992.tb01404.x","DOI":"10.1111\/j.1365-2958.1992.tb01404.x"},{"key":"2026042902430251688_j_pjm-2022-010_ref_023","unstructured":"Soci\u00e9t\u00e9 Fran\u00e7aise de Microbiologie. 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Int J Syst Evol Microbiol. 2014 Aug;64(Pt 8):2723\u20132728. https:\/\/doi.org\/10.1099\/ijs.0.063115-0","DOI":"10.1099\/ijs.0.063115-0"}],"container-title":["Polish Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/reference-global.com\/pdf\/10.33073\/pjm-2022-010","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T13:51:54Z","timestamp":1777470714000},"score":1,"resource":{"primary":{"URL":"https:\/\/reference-global.com\/article\/10.33073\/pjm-2022-010"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,27]]},"references-count":29,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2022,2,27]]},"published-print":{"date-parts":[[2022,3,1]]}},"alternative-id":["10.33073\/pjm-2022-010"],"URL":"https:\/\/doi.org\/10.33073\/pjm-2022-010","relation":{},"ISSN":["2544-4646"],"issn-type":[{"value":"2544-4646","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,27]]}}}