{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T13:29:14Z","timestamp":1787146154206,"version":"build-2736575974"},"reference-count":32,"publisher":"Polish Society of Microbiologists","issue":"3","license":[{"start":{"date-parts":[[2024,8,25]],"date-time":"2024-08-25T00:00:00Z","timestamp":1724544000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Disorders of the vaginal microbiota can lead to many complications and affect fertility. This study evaluates the role of\n                    <jats:italic>Lactobacillus<\/jats:italic>\n                    in the vagina and its impact on the incidence of colonization by pathogenic microorganisms, analyzing the results of 1,039 women of reproductive age (18\u201349 years) who underwent a microbiological examination of the reproductive tract in 2020. Samples were examined by microscopy, culture, and NAAT. As the number of\n                    <jats:italic>Lactobacillus<\/jats:italic>\n                    increases, the chance of developing symptoms decreases. In fact, it has been shown that the higher the number of\n                    <jats:italic>Lactobacillus<\/jats:italic>\n                    spp. the less frequently\n                    <jats:italic>Gardnerella vaginalis<\/jats:italic>\n                    and\n                    <jats:italic>Streptococcus<\/jats:italic>\n                    group B are observed. As the concentration of\n                    <jats:italic>Lactobacillus<\/jats:italic>\n                    spp. increases by one category, the risk of\n                    <jats:italic>G. vaginalis<\/jats:italic>\n                    after adjustment to age and pH decreases by 80% (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.001). Similarly, the correlation between\n                    <jats:italic>Lactobacillus<\/jats:italic>\n                    spp\n                    <jats:italic\/>\n                    . and vaginal pH was shown. After adjustment to age, the odds of prevalence pH &gt; 4.5 for people with\n                    <jats:italic>Lactobacillus<\/jats:italic>\n                    category higher 1 is 76% lower.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:fig id=\"j_pjm-2024-024_fig_001\" position=\"anchor\" fig-type=\"figure\">\n                      <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/j_pjm-2024-024_fig_001.jpg\"\/>\n                    <\/jats:fig>\n                  <\/jats:p>","DOI":"10.33073\/pjm-2024-024","type":"journal-article","created":{"date-parts":[[2024,8,25]],"date-time":"2024-08-25T05:42:44Z","timestamp":1724564564000},"page":"265-273","source":"Crossref","is-referenced-by-count":1,"title":["The Presence of\n                    <i>Lactobacillus<\/i>\n                    spp. and its Effect on the Occurrence of Other Microorganisms in the Reproductive Tract of Polish Women"],"prefix":"10.33073","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3818-8972","authenticated-orcid":false,"given":"Aneta","family":"Kiecka","sequence":"first","affiliation":[{"name":"Jagiellonian University Medical College, Faculty of Health Sciences, Institute of Physiotherapy, Chair of Biomedical Sciences , Cracow , Poland"},{"name":"Centre of Microbiological Research , Cracow , Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4634-619X","authenticated-orcid":false,"given":"Katarzyna","family":"Rak","sequence":"additional","affiliation":[{"name":"Centre of Microbiological Research , Cracow , Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0820-3174","authenticated-orcid":false,"given":"Joanna","family":"Bia\u0142ecka","sequence":"additional","affiliation":[{"name":"Centre of Microbiological Research , Cracow , Poland"},{"name":"Jagiellonian University Medical College, Department of Pharmaceutical Microbiology , Cracow , Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2772-6787","authenticated-orcid":false,"given":"Anna","family":"Bia\u0142ecka","sequence":"additional","affiliation":[{"name":"Centre of Microbiological Research , Cracow , Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0388-2382","authenticated-orcid":false,"given":"Marian","family":"Szczepanik","sequence":"additional","affiliation":[{"name":"Jagiellonian University Medical College, Faculty of Health Sciences, Institute of Physiotherapy, Chair of Biomedical Sciences , Cracow , Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"18083","published-online":{"date-parts":[[2024,8,25]]},"reference":[{"key":"2026081913071095141_j_pjm-2024-024_ref_001","doi-asserted-by":"crossref","unstructured":"Amabebe E, Anumba DOC. The vaginal microenvironment: The physiologic role of Lactobacilli. Front Med. 2018 Jun;5:181. https:\/\/doi.org\/10.3389\/fmed.2018.00181","DOI":"10.3389\/fmed.2018.00181"},{"key":"2026081913071095141_j_pjm-2024-024_ref_002","doi-asserted-by":"crossref","unstructured":"Amsel R, Totten PA, Spiegel CA, Chen KC, Eschenbach D, Holmes KK. Nonspecific vaginitis. Diagnostic criteria and microbial and epidemiologic associations. Am J Med. 1983 Jan;74(1):14\u201322. https:\/\/doi.org\/10.1016\/0002-9343(83)91112-9","DOI":"10.1016\/0002-9343(83)91112-9"},{"key":"2026081913071095141_j_pjm-2024-024_ref_003","doi-asserted-by":"crossref","unstructured":"Aroutcheva A, Gariti D, Simon M, Shott S, Faro J, Simoes JA, Gurguis A, Faro S. Defense factors of vaginal lactobacilli. 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