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We investigated antibiotics combination with proton pump inhibitors against oral clinical isolates. The strains were identified as\n                    <jats:italic>Staphylococcus epidermidis<\/jats:italic>\n                    and\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    by the 16S rRNA gene sequencing. In molecular docking, ciprofloxacin, levofloxacin, and omeprazole best fit to active pockets of transcriptional regulators 4BXI and 3QP1. None of the proton pump inhibitors were active against\n                    <jats:italic>S. epidermidis<\/jats:italic>\n                    , whereas omeprazole showed significant inhibition (MIC 3.9 \u03bcg\/ml). Fluoroquinolones were active against both\n                    <jats:italic>S. epidermidis<\/jats:italic>\n                    and\n                    <jats:italic>S. aureus<\/jats:italic>\n                    . In combination analysis, a marked decrease in minimum inhibitory concentration was noticed with omeprazole (MIC 0.12 \u03bcg\/ml). In antiquorum sensing experiments, a significant inhibitory zone was shown for all fluoroquinolones (14\u201320 mm), whereas among proton pump inhibitors, only omeprazole (12 \u00b1 0.12 mm) was active against\n                    <jats:italic>Chromobacterium violaceum<\/jats:italic>\n                    . In combination analysis, a moderate increase in antiquorum sensing activity was recorded for ciprofloxacin, ofloxacin, and proton pump inhibitors. Further, significant\n                    <jats:italic>S. aureus<\/jats:italic>\n                    biofilm eradication was recorded using of ciprofloxacin, levofloxacin, and omeprazole combination (78 \u00b1 2.1%). The time-kill kinetic studies indicated a bactericidal effect by ciprofloxacin: levofloxacin: omeprazole combination over 24 hrs. It was concluded that fluoroquinolone combined with omeprazole could be an effective treatment option for eradicating oral bacterial biofilms.\n                  <\/jats:p>","DOI":"10.33073\/pjm-2024-028","type":"journal-article","created":{"date-parts":[[2024,9,13]],"date-time":"2024-09-13T07:58:57Z","timestamp":1726214337000},"page":"329-342","source":"Crossref","is-referenced-by-count":3,"title":["Activity of Fluoroquinolones and Proton Pump Inhibitors against Resistant Oral Bacterial Biofilms,\n                    <i>in silico<\/i>\n                    and\n                    <i>in vitro<\/i>\n                    Analysis"],"prefix":"10.33073","volume":"73","author":[{"given":"Muhammad","family":"Kamran","sequence":"first","affiliation":[{"name":"Natural Products Research Lab (NPRL), Gomal Centre of Pharmaceutical Sciences, Faculty of Pharmacy, Gomal University , Dera Ismail Khan , Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Raza","sequence":"additional","affiliation":[{"name":"Peshawar Dental College, Riphah International University , Islamabad , Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riaz","family":"Ullah","sequence":"additional","affiliation":[{"name":"Medicinal Aromatic and Poisonous Plants Research Centre, College of Pharmacy, King Saud University , Riyadh , Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amal","family":"Alotaibi","sequence":"additional","affiliation":[{"name":"Department of Basic Science, College of Medicine, Princess Nourah Bint Abdulrahman University , Riyadh , Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R\u00e0heela","family":"Bano","sequence":"additional","affiliation":[{"name":"Department of Pathology, Gomal Medical College , Dera Ismail Khan , Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Zaman","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, Faculty of Veterinary and Animal Sciences, Gomal University , Dera Ismail Khan , Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sadia","family":"Chaman","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Sciences, UVAS , Lahore , Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kashif","family":"Iqbal","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Ibadat International University , Islamabad , Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahid","family":"Rasool","sequence":"additional","affiliation":[{"name":"College of Pharmacy, University of Sargodha , Sargodha , Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adnan","family":"Amin","sequence":"additional","affiliation":[{"name":"Natural Products Research Lab (NPRL), Gomal Centre of Pharmaceutical Sciences, Faculty of Pharmacy, Gomal University , Dera Ismail Khan , Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"18083","published-online":{"date-parts":[[2024,9,13]]},"reference":[{"key":"2026081913071161683_j_pjm-2024-028_ref_001","doi-asserted-by":"crossref","unstructured":"Appiah T, Boakye YD, Agyare C. Antimicrobial activities and timekill kinetics of extracts of selected Ghanaian mushrooms. Evid Based Complement Alternat Med. 2017;2017:4534350. https:\/\/doi.org\/10.1155\/2017\/4534350","DOI":"10.1155\/2017\/4534350"},{"key":"2026081913071161683_j_pjm-2024-028_ref_002","doi-asserted-by":"crossref","unstructured":"Ball P. Quinolone generations: Natural history or natural selection? J Antimicrob Chemother. 2000 Jul;46(Suppl_3):17\u201324. https:\/\/doi.org\/10.1093\/oxfordjournals.jac.a020889","DOI":"10.1093\/oxfordjournals.jac.a020889"},{"key":"2026081913071161683_j_pjm-2024-028_ref_003","doi-asserted-by":"crossref","unstructured":"Batool M, Keating C, Javed S, Nasir A, Muddassar M, Ijaz UZ. A cross-sectional study of potential antimicrobial resistance and ecology in gastrointestinal and oral microbial communities of young normoweight Pakistani individuals. 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