{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T15:18:18Z","timestamp":1792682298908,"version":"4.5.0"},"reference-count":39,"publisher":"Polish Society of Microbiologists","issue":"4","license":[{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The STI CNM Real-Time PCR Kit from Vitro S.A. (Spain) demonstrates high sensitivity and specificity, is cost-effective, and can detect the three main etiological agents of urethritis\/cervicitis in a single multiplex PCR. Sexually transmitted infections (STIs) are a significant public health problem and a significant burden of morbidity and mortality in hospitals. The World Health Organization (WHO) estimates the number of daily infections to be 1 million. Currently, the number of infections and antimicrobial-resistant strains is rising. A rapid and accurate etiologic diagnosis is critical to solving this problem. In this study, we compared the STI CNM Real-Time PCR Kit using the Xpert\n                    <jats:sup>\u00ae<\/jats:sup>\n                    CT\/NG technique (Cepheid\n                    <jats:sup>\u00ae<\/jats:sup>\n                    , USA) as Gold Standard for the diagnosis of\n                    <jats:italic>Chlamydia trachomatis<\/jats:italic>\n                    and\n                    <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n                    and EasyNAT\n                    <jats:sup>\u00ae<\/jats:sup>\n                    MG (Ustar Biotechnologies (Hangzhou) Ltd., China) as Gold Standard for the diagnosis of\n                    <jats:italic>Mycoplasma genitalium<\/jats:italic>\n                    infection. Regarding\n                    <jats:italic>C. trachomatis<\/jats:italic>\n                    and\n                    <jats:italic>N. gonorrhoeae<\/jats:italic>\n                    , out of 200 samples, there was a match in 199 cases, with only one positive sample not being detected by the STI CNM Real-Time PCR Kit. This results in a sensitivity of 96% and a specificity of 100% for this kit. Diagnosing\n                    <jats:italic>M. genitalium<\/jats:italic>\n                    infection, out of 200 samples, the STI CNM Real-Time PCR Kit correctly detected all negative and positive samples, with 100% agreement compared to the reference technique. In summary, the STI assay has a very high sensitivity and specificity, comparable to other commercial diagnostic kits. Furthermore, it has the advantage of bundling the detection of the three main bacterial agents of urethritis\/cervicitis, resulting in better cost efficiency.\n                  <\/jats:p>","DOI":"10.33073\/pjm-2024-045","type":"journal-article","created":{"date-parts":[[2024,12,13]],"date-time":"2024-12-13T08:03:32Z","timestamp":1734077012000},"page":"529-534","source":"Crossref","is-referenced-by-count":3,"title":["Advantages of Syndromic Diagnostics: Detection of the Pathogens Causing Urethritis\/Cervicitis with the STI CNM Real-Time PCR Kit from Vitro S.A."],"prefix":"10.33073","volume":"73","author":[{"given":"Miguel \u00c1ngel Casta\u00f1o","family":"L\u00f3pez","sequence":"first","affiliation":[{"name":"Clinical Laboratory Department, Hospital Comarcal Infanta Elena , Huelva , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9ctor Toledo","family":"Porteros","sequence":"additional","affiliation":[{"name":"Clinical Laboratory Department, Hospital Comarcal Infanta Elena , Huelva , Spain"},{"name":"Clinical Laboratory Department, La Merced University Hospital , Osuna , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"De La Iglesia Salgado","sequence":"additional","affiliation":[{"name":"Clinical Laboratory Department, Hospital Comarcal Infanta Elena , Huelva , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josefa Vazquez","family":"Medel","sequence":"additional","affiliation":[{"name":"Clinical Laboratory Department, Hospital Comarcal Infanta Elena , Huelva , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inmaculada Garc\u00eda","family":"Borrero","sequence":"additional","affiliation":[{"name":"Clinical Laboratory Department, Hospital Comarcal Infanta Elena , Huelva , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"18083","published-online":{"date-parts":[[2024,12,13]]},"reference":[{"key":"2026081913071570582_j_pjm_2024_045_ref_001","doi-asserted-by":"crossref","unstructured":"Bristow CC, McGrath MR, Cohen AC, Anderson LJ, Gordon KK, Klausner JD. 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