{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T13:29:14Z","timestamp":1787146154593,"version":"build-2736575974"},"reference-count":14,"publisher":"Polish Society of Microbiologists","issue":"3","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    is a bacterium sensitive to desiccation. Its use in disinfectant testing methods is important given its association with hospital-acquired infections. According to PNEN 14561:2008, obtaining a sufficient number of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    cells on a glass test surface under clean conditions after the drying process is essential for evaluating the bactericidal effectiveness of disinfectants. Does reducing the drying temperature of glass carriers contaminated with\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    under clean conditions from the standard-recommended 37\u00b0C \u00b1 1\u00b0C to 24\u00b0C \u00b1 1\u00b0C improve bacterial recovery after drying? The recovery of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    from glass surfaces after drying was performed by the procedure specified for the water control, as outlined in PN-EN 14561:2008. Additionally, in the 24\u00b0C \u00b1 1\u00b0C, the glass carriers were inoculated onto TSA medium, whereas at 37 \u00b0C \u00b1 1\u00b0C the number of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    was determined in hard water following the contact time. The mean recovery of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    (6.37 log \u00b1 0.12 log) was significantly higher in the 24\u00b0C \u00b1 1\u00b0C variant compared to the 37\u00b0C \u00b1 1\u00b0C variant (5.60 log \u00b1 0.24 log). At 24\u00b0C \u00b1 1\u00b0C,\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    remained on the glass carrier after recovery, whereas at 37\u00b0C \u00b1 1\u00b0C, the bacteria were detected in hard water. Lowering the drying temperature did not allow recovery of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    at the level required by PN-EN 14561:2008. The reduction in\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    recovery resulted from bacterial detachment into hard water and retention on the carrier surface.\n                  <\/jats:p>","DOI":"10.33073\/pjm-2025-034","type":"journal-article","created":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T01:07:10Z","timestamp":1758244030000},"page":"401-406","source":"Crossref","is-referenced-by-count":0,"title":["Influence of Drying Condition and Other Aspects on Recovery of\n                    <i>Pseudomonas aeruginosa<\/i>\n                    in the PN-EN 14561 Carrier Test"],"prefix":"10.33073","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6311-2961","authenticated-orcid":false,"given":"AGNIESZKA","family":"CHOJECKA","sequence":"first","affiliation":[{"name":"Department of Bacteriology and Biocontamination Control; National Institute of Public Health NIH \u2013 National Research Institute , Warsaw , Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"18083","published-online":{"date-parts":[[2025,9,16]]},"reference":[{"key":"2026081913072873647_j_pjm-2025-034_ref_001","doi-asserted-by":"crossref","unstructured":"Boyd A, Chakrabarty AM. Pseudomonas aeruginosa biofilms: Role of the alginate exopolysaccharide. J Ind Microbiol. 1995 Sep;15(3):162\u2013168. https:\/\/doi.org\/10.1007\/bf01569821","DOI":"10.1007\/BF01569821"},{"key":"2026081913072873647_j_pjm-2025-034_ref_002","doi-asserted-by":"crossref","unstructured":"Couchoud C, Bertrand X, Bourgeon M, Piton G, Valot B, Hocquet D. Genome-based typing reveals rare events of patient contamination with Pseudomonas aeruginosa from other patients and sink traps in a medical intensive care unit. J Hosp Infect. 2023 Apr;134:63\u201370. https:\/\/doi.org\/10.1016\/j.jhin.2023.01.010","DOI":"10.1016\/j.jhin.2023.01.010"},{"key":"2026081913072873647_j_pjm-2025-034_ref_003","doi-asserted-by":"crossref","unstructured":"Diggle SP, Whiteley M. Microbe profile: Pseudomonas aeruginosa: Opportunistic pathogen and lab rat. 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Chemical disinfectants and antiseptics. Quantitative carrier test for the evaluation of bactericidal activity for instruments used in the medical area. Test method and requirements (phase 2, step 2). Warsaw (Poland): Polish Committee for Standardization; 2008."},{"key":"2026081913072873647_j_pjm-2025-034_ref_010","unstructured":"PN-EN 14885:2022-09. Chemical disinfectants and antiseptics. Application of European Standards for chemical disinfectants and antiseptics. Warsaw (Poland): Polish Committee for Standardization; 2022."},{"key":"2026081913072873647_j_pjm-2025-034_ref_011","doi-asserted-by":"crossref","unstructured":"Rozman U, Pu\u0161nik M, Kmetec S, Duh D, \u0160ostar Turk S. Reduced susceptibility and increased resistance of bacteria against disinfectants: A systematic review. 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