{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T13:29:17Z","timestamp":1787146157712,"version":"build-2736575974"},"reference-count":21,"publisher":"Polish Society of Microbiologists","issue":"2","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Bloodstream infections cause significant morbidity and mortality, and rapid identification of the causative microorganisms, along with their antimicrobial resistance profiles, is crucial for appropriate treatment. Molecular diagnostic systems such as the BioFire FilmArray Blood Culture Identification 2 (BCID2) panel provide faster results than conventional culture-based methods. This study evaluated the performance of the BCID2 panel for rapid detection of microorganisms and antimicrobial resistance genes in 50 positive blood cultures collected at a university hospital between January 2023 and September 2025. All samples were obtained from peripheral blood cultures, and repeat or catheter-related cultures were excluded. Samples were analyzed using conventional culture methods, mass spectrometry-based identification, and automated antimicrobial susceptibility testing, in parallel with the BCID2 panel. Resistance genes detected by the panel were confirmed using polymerase chain reaction. At least one microorganism was detected in forty-seven samples (94%), showing strong agreement between molecular and culture-based methods. Complete concordance was observed for\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    and\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    , while\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    showed slightly lower but acceptable agreement. Polymicrobial results were detected by BCID2 in 20 cases (40%). Detected resistance genes correlated well with phenotypic resistance results. However, the absence of clinical correlation data and repeat blood culture information limits the interpretation of these findings, particularly for coagulase-negative staphylococci. The BCID2 panel enables rapid and reliable identification of bloodstream pathogens and relevant resistance genes, serving as a valuable complement to conventional diagnostic methods when interpreted alongside clinical data.\n                  <\/jats:p>","DOI":"10.33073\/pjm-2026-020","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T11:57:44Z","timestamp":1782820664000},"page":"220-226","source":"Crossref","is-referenced-by-count":0,"title":["Rapid Detection of Pathogens and Resistance Genes in Positive Blood Cultures Using BioFire\n                    <sup>\u00ae<\/sup>\n                    FilmArray BCID2"],"prefix":"10.33073","volume":"75","author":[{"given":"Canberk","family":"\u00c7inar","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine, Ondokuz May\u0131s University , Samsun , Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeliz Tanriverdi","family":"\u00c7ayci","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine, Ondokuz May\u0131s University , Samsun , Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zehranur","family":"Atakcan","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine, Ondokuz May\u0131s University , Samsun , Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u0130lknur","family":"Biyik","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine, Ondokuz May\u0131s University , Samsun , Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kemal","family":"Bilgin","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine, Ondokuz May\u0131s University , Samsun , Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Demet G\u00fcr","family":"Vural","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine, Ondokuz May\u0131s University , Samsun , Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asuman","family":"Birinci","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine, Ondokuz May\u0131s University , Samsun , Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"18083","published-online":{"date-parts":[[2026,6,29]]},"reference":[{"key":"2026081913073902546_j_pjm-2026-020_ref_001","doi-asserted-by":"crossref","unstructured":"Banerjee R, Teng CB, Cunningham SA, Ihde SM, Steckelberg JM, Moriarty JP, Patel R. 2015. Randomized trial of rapid multiplex polymerase chain reaction\u2013based blood culture identification and susceptibility testing. Clin Infect Dis. 61(7):1071\u20131080. https:\/\/doi.org\/10.1093\/cid\/civ447","DOI":"10.1093\/cid\/civ447"},{"key":"2026081913073902546_j_pjm-2026-020_ref_002","doi-asserted-by":"crossref","unstructured":"Beekmann SE, Diekema DJ, Chapin KC, Doern GV. 2003. Effects of rapid detection of bloodstream infections on length of hospitalization and hospital charges. J Clin Microbiol. 41(7):3119\u20133125. https:\/\/doi.org\/10.1128\/JCM.41.7.3119-3125.2003","DOI":"10.1128\/JCM.41.7.3119-3125.2003"},{"key":"2026081913073902546_j_pjm-2026-020_ref_003","doi-asserted-by":"crossref","unstructured":"Briggs N, Campbell S, Gupta S. 2021. Advances in rapid diagnostics for bloodstream infections. Diagn Microbiol Infect Dis. 99(1):115219. https:\/\/doi.org\/10.1016\/j.diagmicrobio.2020.115219","DOI":"10.1016\/j.diagmicrobio.2020.115219"},{"key":"2026081913073902546_j_pjm-2026-020_ref_004","doi-asserted-by":"crossref","unstructured":"Daneman N, Rishu AH, Pinto R, Aslanian P, Fowler RA, Powis J, Bell CM, Cook DJ, Marshall JC, Heyland DK. 2025. Antibiotic treatment for 7 versus 14 days in patients with bloodstream infections. N Engl J Med. 392(11):1065\u20131078. https:\/\/doi.org\/10.1056\/NEJMoa2404991","DOI":"10.1056\/NEJMoa2404991"},{"key":"2026081913073902546_j_pjm-2026-020_ref_005","doi-asserted-by":"crossref","unstructured":"Elzi L, Babouee B, V\u00f6geli N, Laffer R, Dangel M, Frei R, Widmer AF, Battegay M, Fl\u00fcckiger U. 2012. How to discriminate contamination from bloodstream infection due to coagulase-negative staphylococci: a prospective study with 654 patients. 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