{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T22:53:18Z","timestamp":1787352798289,"version":"build-2736575974"},"reference-count":33,"publisher":"The Japanese Society for Medical Mycology","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medical Mycology Journal"],"published-print":{"date-parts":[[2018]]},"DOI":"10.3314\/mmj.17-00016","type":"journal-article","created":{"date-parts":[[2018,2,27]],"date-time":"2018-02-27T22:25:13Z","timestamp":1519770313000},"page":"E7-E18","source":"Crossref","is-referenced-by-count":9,"title":["Histopathological Diagnosis of Invasive Fungal Infections in Formalin-Fixed and Paraffin-Embedded Tissues in Conjunction with Molecular Methods"],"prefix":"10.3314","volume":"59","author":[{"given":"Minoru","family":"Shinozaki","sequence":"first","affiliation":[{"name":"Department of Pathology, Toho University Graduate School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naobumi","family":"Tochigi","sequence":"additional","affiliation":[{"name":"Department of Pathology, Toho University Graduate School of Medicine"},{"name":"Department of Surgical Pathology, Toho University School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sota","family":"Sadamoto","sequence":"additional","affiliation":[{"name":"Department of Pathology, Toho University Graduate School of Medicine"},{"name":"Department of Surgical Pathology, Toho University School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Somay","family":"Yamagata Murayama","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Cell Biology, School of Pharmacy, Nihon University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megumi","family":"Wakayama","sequence":"additional","affiliation":[{"name":"Department of Pathology, Toho University Graduate School of Medicine"},{"name":"Department of Surgical Pathology, Toho University School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuo","family":"Nemoto","sequence":"additional","affiliation":[{"name":"Department of Surgical Pathology, Toho University School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1842","reference":[{"key":"1","unstructured":"1) Pagano L, Caira M, Candoni A, et al: The epidemiology of fungal infections in patients with hematologic malignancies: the SEIFEM-2004 study. Haematologica 91: 1068-1075, 2006."},{"key":"2","doi-asserted-by":"crossref","unstructured":"2) Infectious Diseases Working Party (AGIHO) of the German Society of Hematology and Oncology (DGHO), B&ouml;hme A, Ruhnke M, et al: Treatment of invasive fungal infections in cancer patients&mdash;recommendations of the Infectious Diseases Working Party (AGIHO) of the German Society of Hematology and Oncology (DGHO). Ann Hematol 88: 97-110, 2009.","DOI":"10.1007\/s00277-008-0622-5"},{"key":"3","doi-asserted-by":"crossref","unstructured":"3) Shimodaira K, Okubo Y, Nakayama H, Wakayama M, Shinozaki M, Ishiwatari T, Sasai D, Nemoto T, Takahashi K, Ishii T, Saji T, Shibuya K: Trends in the prevalence of invasive fungal infections from an analysis of annual records of autopsy cases of Toho University. Mycoses 55: 435-443, 2012.","DOI":"10.1111\/j.1439-0507.2012.02169.x"},{"key":"4","doi-asserted-by":"crossref","unstructured":"4) Vennewald I, Henker M, Klemm E, Seebacher C: Fungal colonization of the paranasal sinuses. Mycoses 42 Suppl 2: 33-36, 1999.","DOI":"10.1111\/j.1439-0507.1999.tb00010.x"},{"key":"5","doi-asserted-by":"crossref","unstructured":"5) Thaler M, Pastakia B, Shawker TH, O&rsquo;Leary T, Pizzo PA: Hepatic candidiasis in cancer patients: the evolving picture of the syndrome. Ann Intern Med 108: 88-100, 1988.","DOI":"10.7326\/0003-4819-108-1-88"},{"key":"6","doi-asserted-by":"crossref","unstructured":"6) Kahn FW, Jones JM, England DM: The role of bronchoalveolar lavage in the diagnosis of invasive pulmonary aspergillosis. Am J Clin Pathol 86: 518-523, 1986.","DOI":"10.1093\/ajcp\/86.4.518"},{"key":"7","doi-asserted-by":"crossref","unstructured":"7) Groll AH, Shah PM, Mentzel C, Schneider M, Just-Nuebling G, Huebner K: Trends in the postmortem epidemiology of invasive fungal infections at a university hospital. J Infect 33: 23-32, 1996.","DOI":"10.1016\/S0163-4453(96)92700-0"},{"key":"8","doi-asserted-by":"crossref","unstructured":"8) Shinozaki M, Tochigi N, Sadamoto S, Yamagata Murayama S, Wakayama M, Nemoto T, Shibuya K: Technical aspects and applications for developing <I>in situ<\/I> hybridization procedures for formalin-fixed and paraffin-embedded (FFPE) tissues for diagnosis of fungal infections. Med Mycol J 58: E33-E37, 2017.","DOI":"10.3314\/mmj.16-00025"},{"key":"9","doi-asserted-by":"crossref","unstructured":"9) Shinozaki M, Okubo Y, Sasai D, Nakayama H, Ishiwatari T, Murayama S, Tochigi N, Wakayama M, Nemoto T, Shibuya K: Development and evaluation of nucleic acid-based techniques for an auxiliary diagnosis of invasive fungal infections in formalin-fixed and paraffin-embedded (FFPE) tissues. Med Mycol J 53: 241-245, 2012.","DOI":"10.3314\/mmj.53.241"},{"key":"10","doi-asserted-by":"crossref","unstructured":"10) Shinozaki M, Okubo Y, Sasai D, Nakayama H, Murayama SY, Ide T, Wakayama M, Hiruta N, Shibuya K: Identification of <I>Fusarium<\/I> species in formalin-fixed and paraffin-embedded sections by <I>in situ<\/I> hybridization using peptide nucleic acid probes. J Clin Microbiol 49: 808-813, 2011.","DOI":"10.1128\/JCM.01149-10"},{"key":"11","doi-asserted-by":"crossref","unstructured":"11) Seng&uuml;ven B, Baris E, Oygur T, Berktas M: Comparison of methods for the extraction of DNA from formalin-fixed, paraffin-embedded archival tissues. Int J Med Sci 11: 494-499, 2014.","DOI":"10.7150\/ijms.8842"},{"key":"12","doi-asserted-by":"crossref","unstructured":"12) Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA: Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239: 487-491, 1988.","DOI":"10.1126\/science.2448875"},{"key":"13","doi-asserted-by":"crossref","unstructured":"13) Willinger B, Obradovic A, Selitsch B, Beck-Mannagetta J, Buzina W, Braun H, Apfalter P, Hirschl AM, Makristathis A, Rotter M: Detection and identification of fungi from fungus balls of the maxillary sinus by molecular techniques. J Clin Microbiol 41: 581-585, 2003.","DOI":"10.1128\/JCM.41.2.581-585.2003"},{"key":"14","doi-asserted-by":"crossref","unstructured":"14) Makimura K, Tamura Y, Mochizuki T, Hasegawa A, Tajiri Y, Hanazawa R, Uchida K, Saito H, Yamaguchi H: Phylogenetic classification and species identification of dermatophyte strains based on DNA sequences of nuclear ribosomal internal transcribed spacer 1 regions. J Clin Microbiol 37: 920-924, 1999.","DOI":"10.1128\/JCM.37.4.920-924.1999"},{"key":"15","doi-asserted-by":"crossref","unstructured":"15) Skladny H, Buchheidt D, Baust C, Krieg-Schneider F, Seifarth W, Leib-M&ouml;sch C, Hehlmann R: Specific detection of <I>Aspergillus<\/I> species in blood and bronchoalveolar lavage samples of immunocompromised patients by two-step PCR. J Clin Microbiol 37: 3865-3871, 1999.","DOI":"10.1128\/JCM.37.12.3865-3871.1999"},{"key":"16","doi-asserted-by":"crossref","unstructured":"16) Hofman V, Dhouibi A, Butori C, Padovani B, Gari-Toussaint M, Garcia-Hermoso D, Baumann M, V&eacute;nissac N, Cathomas G, Hofman P: Usefulness of molecular biology performed with formaldehyde-fixed paraffin embedded tissue for the diagnosis of combined pulmonary invasive mucormycosis and aspergillosis in an immunocompromised patient. Diagn Pathol 5: 1, 2010.","DOI":"10.1186\/1746-1596-5-1"},{"key":"17","doi-asserted-by":"crossref","unstructured":"17) Bialek R, Konrad F, Kern J, Aepinus C, Cecenas L, Gonzalez GM, Just-N&uuml;bling G, Willinger B, Presterl E, Lass-Fl&ouml;rl C, Rickerts V: PCR based identification and discrimination of agents of mucormycosis and aspergillosis in paraffin wax embedded tissue. J Clin Pathol 58: 1180-1184, 2005.","DOI":"10.1136\/jcp.2004.024703"},{"key":"18","doi-asserted-by":"crossref","unstructured":"18) Hammond SP, Bialek R, Milner DA, Petschnigg EM, Baden LR, Marty FM: Molecular methods to improve diagnosis and identification of mucormycosis. J Clin Microbiol 49: 2151-2153, 2011.","DOI":"10.1128\/JCM.00256-11"},{"key":"19","doi-asserted-by":"crossref","unstructured":"19) Hagari Y, Ishioka S, Ohyama F, Mihara M: Cutaneous infection showing sporotrichoid spread caused by Pseudallescheria boydii (Scedosporium apiospermum): successful detection of fungal DNA in formalin-fixed, paraffin-embedded sections by seminested PCR. Arch Dermatol 138: 271-272, 2002.","DOI":"10.1001\/archderm.138.2.271"},{"key":"20","doi-asserted-by":"crossref","unstructured":"20) Zhi Y, Sasai D, Okubo Y, Shinozaki M, Nakayama H, Yamagata Murayama S, Wakayama M, Ide T, Zhang Z, Shibuya K: Comparison between the effectiveness of polymerase chain reaction and <I>in situ<\/I> hybridization in detecting the presence of pathogenic fungi by using the preserved DNA in formalin-fixed and paraffin-embedded tissues. Jpn J Infect Dis 66: 173-179, 2013.","DOI":"10.7883\/yoken.66.173"},{"key":"21","doi-asserted-by":"crossref","unstructured":"21) Hanazawa R, Murayama SY, Yamaguchi H: <I>In-situ<\/I> detection of <I>Aspergillus fumigatus<\/I>. J Med Microbiol 49: 285-290, 2000.","DOI":"10.1099\/0022-1317-49-3-285"},{"key":"22","doi-asserted-by":"crossref","unstructured":"22) Okubo Y, Shinozaki M, Wakayama M, Nakayama H, Sasai D, Ishiwatari T, Nemoto T, Tochigi N, Shibuya K: Applied gene histopathology: identification of <I>Fusarium<\/I> species in FFPE tissue sections by <I>in situ<\/I> hybridization. Methods Mol Biol 968: 141-147, 2013.","DOI":"10.1007\/978-1-62703-257-5_10"},{"key":"23","doi-asserted-by":"crossref","unstructured":"23) Shinozaki M, Okubo Y, Sasai D, Nakayama H, Murayama SY, Ide T, Wakayama M, Ishiwatari T, Tochigi N, Nemoto T, Shibuya K: Development of a peptide nucleic acid probe to <I>Trichosporon<\/I> species and identification of trichosporonosis by use of <I>in situ<\/I> hybridization in formalin-fixed and paraffin-embedded (FFPE) sections. J Clin Microbiol 51: 295-298, 2013.","DOI":"10.1128\/JCM.02221-12"},{"key":"24","doi-asserted-by":"crossref","unstructured":"24) Oliveira K, Haase G, Kurtzman C, Hyldig-Nielsen JJ, Stender H: Differentiation of <I>Candida albicans<\/I> and <I>Candida dubliniensis<\/I> by fluorescent <I>in situ<\/I> hybridization with peptide nucleic acid probes. J Clin Microbiol 39: 4138-4141, 2001.","DOI":"10.1128\/JCM.39.11.4138-4141.2001"},{"key":"25","doi-asserted-by":"crossref","unstructured":"25) Yeo SF, Wong B: Current status of nonculture methods for diagnosis of invasive fungal infections. Clin Microbiol Rev 15: 465-484, 2002.","DOI":"10.1128\/CMR.15.3.465-484.2002"},{"key":"26","doi-asserted-by":"crossref","unstructured":"26) Sangoi AR, Rogers WM, Longacre TA, Montoya JG, Baron EJ, Banaei N: Challenges and pitfalls of morphologic identification of fungal infections in histologic and cytologic specimens: a ten-year retrospective review at a single institution. Am J Clin Pathol 131: 364-375, 2009.","DOI":"10.1309\/AJCP99OOOZSNISCZ"},{"key":"27","doi-asserted-by":"crossref","unstructured":"27) Shinozaki M, Okubo Y, Nakayama H, Mitsuda A, Ide T, Yamagata Murayama S, Shibuya K: Application of <I>in situ<\/I> hybridization to tissue sections for identification of molds causing invasive fungal infection. Nihon Ishinkin Gakkai Zasshi 50: 75-83, 2009.","DOI":"10.3314\/jjmm.50.075"},{"key":"28","doi-asserted-by":"crossref","unstructured":"28) Lau A, Chen S, Sorrell T, Carter D, Malik R, Martin P, Halliday C: Development and clinical application of a panfungal PCR assay to detect and identify fungal DNA in tissue specimens. J Clin Microbiol 45: 380-385, 2007.","DOI":"10.1128\/JCM.01862-06"},{"key":"29","doi-asserted-by":"crossref","unstructured":"29) Moncada PA, Budvytiene I, Ho DY, Deresinski SC, Montoya JG, Banaei N: Utility of DNA sequencing for direct identification of invasive fungi from fresh and formalin-fixed specimens. Am J Clin Pathol 140: 203-208, 2013.","DOI":"10.1309\/AJCPNSU2SDZD9WPW"},{"key":"30","doi-asserted-by":"crossref","unstructured":"30) Walsh TJ, Groll A, Hiemenz J, Fleming R, Roilides E, Anaissie E: Infections due to emerging and uncommon medically important fungal pathogens. Clin Microbiol Infect 10: 48-66, 2004.","DOI":"10.1111\/j.1470-9465.2004.00839.x"},{"key":"31","unstructured":"31) Dedhia P, Tarale S, Dhongde G, Khadapkar R, Das B: Evaluation of DNA extraction methods and real time PCR optimization on formalin-fixed paraffin-embedded tissues. Asian Pac J Cancer Prev 8: 55-59, 2007."},{"key":"32","doi-asserted-by":"crossref","unstructured":"32) Frickmann H, Loderstaedt U, Racz P, Tenner-Racz K, Eggert P, Haeupler A, Bialek R, Hagen RM: Detection of tropical fungi in formalin-fixed, paraffin-embedded tissue: still an indication for microscopy in times of sequence-based diagnosis? Biomed Res Int 2015. doi: 10.1155\/2015\/938721. [Epub ahead of print]","DOI":"10.1155\/2015\/938721"},{"key":"33","doi-asserted-by":"crossref","unstructured":"33) Mu&ntilde;oz-Cadavid C, Rudd S, Zaki SR, Patel M, Moser SA, Brandt ME, G&oacute;mez BL: Improving molecular detection of fungal DNA in formalin-fixed paraffin-embedded tissues: comparison of five tissue DNA extraction methods using panfungal PCR. J Clin Microbiol 48: 2147-2153, 2010.","DOI":"10.1128\/JCM.00459-10"}],"container-title":["Medical Mycology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/mmj\/59\/1\/59_17-00016\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,28]],"date-time":"2020-10-28T20:15:12Z","timestamp":1603916112000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/mmj\/59\/1\/59_17-00016\/_article\/-char\/ja\/"}},"subtitle":["Comparison of Reproducibility and Reliability of Histopathological Evaluation, Polymerase Chain Reaction, and &lt;I&gt;In Situ&lt;\/I&gt; Hybridization"],"short-title":[],"issued":{"date-parts":[[2018]]},"references-count":33,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018]]}},"URL":"https:\/\/doi.org\/10.3314\/mmj.17-00016","relation":{},"ISSN":["2185-6486","2186-165X"],"issn-type":[{"value":"2185-6486","type":"print"},{"value":"2186-165X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018]]}}}