{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T13:51:59Z","timestamp":1780494719044,"version":"3.54.1"},"reference-count":31,"publisher":"The Japanese Society for Medical Mycology","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medical Mycology Journal"],"published-print":{"date-parts":[[2025,7,31]]},"DOI":"10.3314\/mmj.25-00014","type":"journal-article","created":{"date-parts":[[2025,8,30]],"date-time":"2025-08-30T22:08:23Z","timestamp":1756591703000},"page":"131-137","source":"Crossref","is-referenced-by-count":2,"title":["Report of Four Azole-Resistant <i>Aspergillus fumigatus<\/i> Isolates with TR34 or TR46 Mutations in Japan Referred to a Mycosis Reference Center for Examination"],"prefix":"10.3314","volume":"66","author":[{"given":"Teppei","family":"Arai","sequence":"first","affiliation":[{"name":"Division of Clinical Research, Medical Mycology Research Center, Chiba University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidetaka","family":"Majima","sequence":"additional","affiliation":[{"name":"Division of Clinical Research, Medical Mycology Research Center, Chiba University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoto","family":"Maruguchi","sequence":"additional","affiliation":[{"name":"Division of Clinical Research, Medical Mycology Research Center, Chiba University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayaka","family":"Ban","sequence":"additional","affiliation":[{"name":"Management Unit of Microbiological Resources, Medical Mycology Research Center, Chiba University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Yaguchi","sequence":"additional","affiliation":[{"name":"Management Unit of Microbiological Resources, Medical Mycology Research Center, Chiba University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Division of Clinical Research, Medical Mycology Research Center, Chiba University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1842","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"1) Denning DW: Global incidence and mortality of severe fungal disease. Lancet Infect Dis 24: e428–38, 2024.","DOI":"10.1016\/S1473-3099(23)00692-8"},{"key":"2","doi-asserted-by":"crossref","unstructured":"2) Howard SJ, Cerar D, Anderson MJ, Albarrag A, Fisher MC, Pasqualotto AC, et al: Frequency and evolution of Azole resistance in Aspergillus fumigatus<\/i> associated with treatment failure. Emerg Infect Dis 15: 1068–76, 2009.","DOI":"10.3201\/eid1507.090043"},{"key":"3","doi-asserted-by":"crossref","unstructured":"3) Buil JB, Snelders E, Denardi LB, Melchers WJG, Verweij PE: Trends in azole resistance in Aspergillus fumigatus<\/i>, the Netherlands, 1994-2016. Emerg Infect Dis 25: 176–8, 2019.","DOI":"10.3201\/eid2501.171925"},{"key":"4","doi-asserted-by":"crossref","unstructured":"4) Lestrade PPA, Meis JF, Melchers WJG, Verweij PE: Triazole resistance in Aspergillus fumigatus<\/i>: recent insights and challenges for patient management. Clin Microbiol Infect 25: 799–806, 2019.","DOI":"10.1016\/j.cmi.2018.11.027"},{"key":"5","doi-asserted-by":"crossref","unstructured":"5) Tashiro M, Izumikawa K, Hirano K, Ide S, Mihara T, Hosogaya N, et al: Correlation between triazole treatment history and susceptibility in clinically isolated Aspergillus fumigatus<\/i>. Antimicrob Agents Chemother 56: 4870–5, 2012.","DOI":"10.1128\/AAC.00514-12"},{"key":"6","doi-asserted-by":"crossref","unstructured":"6) Hagiwara D: Current status of azole-resistant Aspergillus fumigatus<\/i> isolates in East Asia. Med Mycol J 59: e71–6, 2018.","DOI":"10.3314\/mmj.18.010"},{"key":"7","doi-asserted-by":"crossref","unstructured":"7) Tashiro M, Izumikawa K, Minematsu A, Hirano K, Iwanaga N, Ide S, et al: Antifungal susceptibilities of Aspergillus fumigatus<\/i> clinical isolates obtained in Nagasaki, Japan. Antimicrob Agents Chemother 56: 584–7, 2012.","DOI":"10.1128\/AAC.05394-11"},{"key":"8","doi-asserted-by":"crossref","unstructured":"8) Takeda K, Suzuki J, Watanabe A, Arai T, Koiwa T, Shinfuku K, et al: High detection rate of azole-resistant Aspergillus fumigatus<\/i> after treatment with azole antifungal drugs among patients with chronic pulmonary aspergillosis in a single hospital setting with low azole resistance. Med Mycol 59: 327–34, 2021.","DOI":"10.1093\/mmy\/myaa052"},{"key":"9","doi-asserted-by":"crossref","unstructured":"9) Hagiwara D, Takahashi H, Fujimoto M, Sugahara M, Misawa Y, Gonoi T, et al: Multi-azole resistant Aspergillus fumigatus<\/i> harboring Cyp51A TR46\/Y121F\/T289A isolated in Japan. J Infect Chemother 22: 577–9, 2016.","DOI":"10.1016\/j.jiac.2016.01.015"},{"key":"10","doi-asserted-by":"crossref","unstructured":"10) Onishi K, Muhammad Sarumoh B, Hagiwara D, Watanabe A, Kamei K, Toyotome T: Azole-resistant Aspergillus fumigatus<\/i> containing a 34-bp tandem repeat in cyp51A promoter is isolated from the environment in Japan. Med Mycol J 58: E67–70, 2017.","DOI":"10.3314\/mmj.17-00002"},{"key":"11","unstructured":"11) Clinical and Laboratory Standards Institute: Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous Fungi, 3rd Edition. https:\/\/clsi.org\/standards\/products\/microbiology\/documents\/m38\/"},{"key":"12","doi-asserted-by":"crossref","unstructured":"12) de Valk HA, Meis JF, Curfs IM, Muehlethaler K, Mouton JW, Klaassen CH: Use of a novel panel of nine short tandem repeats for exact and high-resolution fingerprinting of Aspergillus fumigatus<\/i> isolates. J Clin Microbiol 43: 4112–20, 2005.","DOI":"10.1128\/JCM.43.8.4112-4120.2005"},{"key":"13","doi-asserted-by":"crossref","unstructured":"13) Chowdhary A, Kathuria S, Xu J, Sharma C, Sundar G, Singh PK, et al: Clonal expansion and emergence of environmental multiple-triazole-resistant Aspergillus fumigatus<\/i> strains carrying the TR\u2083\u2084\/L98H mutations in the cyp51A gene in India. PLoS One 7: e52871, 2012.","DOI":"10.1371\/journal.pone.0052871"},{"key":"14","doi-asserted-by":"crossref","unstructured":"14) Ahangarkani F, Badali H, Abbasi K, Nabili M, Khodavaisy S, de Groot T, et al: Clonal expansion of environmental triazole resistant Aspergillus fumigatus<\/i> in Iran. J Fungi 6: 199, 2020.","DOI":"10.3390\/jof6040199"},{"key":"15","doi-asserted-by":"crossref","unstructured":"15) Escribano P, Recio S, Pel\u00e1ez T, Bouza E, Guinea J: Aspergillus fumigatus<\/i> strains with mutations in the cyp51A<\/i> gene do not always show phenotypic resistance to itraconazole, voriconazole, or posaconazole. Antimicrob Agents Chemother 55: 2460–2, 2011.","DOI":"10.1128\/AAC.01358-10"},{"key":"16","doi-asserted-by":"crossref","unstructured":"16) Mortensen KL, Jensen RH, Johansen HK, Skov M, Pressler T, Howard SJ, et al: Aspergillus<\/i> species and other molds in respiratory samples from patients with cystic fibrosis: a laboratory-based study with focus on Aspergillus fumigatus<\/i> azole resistance. J Clin Microbiol 49: 2243–51, 2011.","DOI":"10.1128\/JCM.00213-11"},{"key":"17","doi-asserted-by":"crossref","unstructured":"17) Tsuchido Y, Tanaka M, Nakano S, Yamamoto M, Matsumura Y, Nagao M: Prospective multicenter surveillance of clinically isolated Aspergillus<\/i> species revealed azole-resistant Aspergillus fumigatus<\/i> isolates with TR34\/L98H mutation in the Kyoto and Shiga regions of Japan. Med Mycol 57: 997–1003, 2019.","DOI":"10.1093\/mmy\/myz003"},{"key":"18","doi-asserted-by":"crossref","unstructured":"18) Dunne K, Hagen F, Pomeroy N, Meis JF, Rogers TR: Intercountry transfer of triazole-resistant Aspergillus fumigatus<\/i> on plant bulbs. Clin Infect Dis 65: 147–9, 2017.","DOI":"10.1093\/cid\/cix257"},{"key":"19","doi-asserted-by":"crossref","unstructured":"19) Hagiwara D: Isolation of azole-resistant Aspergillus fumigatus<\/i> from imported plant bulbs in Japan and the effect of fungicide treatment. J Pestic Sci 45: 147–50, 2020.","DOI":"10.1584\/jpestics.D20-017"},{"key":"20","doi-asserted-by":"crossref","unstructured":"20) Nakano Y, Tashiro M, Urano R, Kikuchi M, Ito N, Moriya E, et al: Characteristics of azole-resistant Aspergillus fumigatus<\/i> attached to agricultural products imported to Japan. J Infect Chemother 26: 1021–5, 2020.","DOI":"10.1016\/j.jiac.2020.05.008"},{"key":"21","doi-asserted-by":"crossref","unstructured":"21) Cogliati M, Buil JB, Esposto MC, Prigitano A, Roman\u00f2 L, Melchers WJG: Evaluation of environmental factors related to Aspergillus fumigatus<\/i> azole resistance in the Netherlands. Sci Total Environ 958: 177923, 2025.","DOI":"10.1016\/j.scitotenv.2024.177923"},{"key":"22","doi-asserted-by":"crossref","unstructured":"22) Sewell TR, Zhu J, Rhodes J, Hagen F, Meis JF, Fisher, et al: Nonrandom distribution of azole resistance across the global population of Aspergillus fumigatus<\/i>. mBio 10: e00392-19, 2019.","DOI":"10.1128\/mBio.00392-19"},{"key":"23","doi-asserted-by":"crossref","unstructured":"23) Etienne KA, Berkow EL, Gade L, Nunnally N, Lockhart SR, Beer K, et al: Genomic diversity of azole-resistant Aspergillus fumigatus<\/i> in the United States. mBio 12: e0180321, 2021.","DOI":"10.1128\/mBio.01803-21"},{"key":"24","doi-asserted-by":"crossref","unstructured":"24) Rhodes J, Abdolrasouli A, Dunne K, Sewell TR, Zhang Y, Ballard E, et al: Population genomics confirms acquisition of drug-resistant Aspergillus fumigatus<\/i> infection by humans from the environment. Nat Microbiol 7: 663–74, 2022.","DOI":"10.1038\/s41564-022-01091-2"},{"key":"25","doi-asserted-by":"crossref","unstructured":"25) Camps SMT, Rijs AJMM, Klaassen CHW, Meis JF, O\u2019Gorman CM, Dyer PS, et al: Molecular epidemiology of Aspergillus fumigatus<\/i> isolates harboring the TR34\/L98H azole resistance mechanism. J Clin Microbiol 50: 2674–80, 2012.","DOI":"10.1128\/JCM.00335-12"},{"key":"26","doi-asserted-by":"crossref","unstructured":"26) Arendrup MC, Hare RK, J\u00f8rgensen KM, Bollmann UE, Bech TB, Hansen CC, et al: Environmental hot spots and \nresistance-associated application practices for azole-resistant Aspergillus fumigatus<\/i>, Denmark, 2020\u20132023. Emerg Infect Dis 30: 1531–41, 2024.","DOI":"10.3201\/eid3008.240096"},{"key":"27","doi-asserted-by":"crossref","unstructured":"27) Snelders E, van der Lee HAL, Kuijpers J, Rijs AJMM, Varga J, Samson RA, et al: Emergence of azole resistance in Aspergillus fumigatus<\/i> and spread of a single resistance mechanism. PLoS Med 5: e219, 2008.","DOI":"10.1371\/journal.pmed.0050219"},{"key":"28","doi-asserted-by":"crossref","unstructured":"28) Meis JF, Chowdhary A, Rhodes JL, Fisher MC, Verweij PE: Clinical implications of globally emerging azole resistance in Aspergillus fumigatus<\/i>. Philos Trans R Soc Lond B Biol Sci 371: 20150460, 2016.","DOI":"10.1098\/rstb.2015.0460"},{"key":"29","doi-asserted-by":"crossref","unstructured":"29) Verweij PE, Chowdhary A, Melchers WJG, Meis JF: Azole resistance in Aspergillus fumigatus<\/i>: Can we retain the clinical use of mold-active antifungal azoles? Clin Infect Dis 62: 362–8, 2016.","DOI":"10.1093\/cid\/civ885"},{"key":"30","doi-asserted-by":"crossref","unstructured":"30) Verweij PE, Zhang J, Debets AJM, Meis JF, van de Veerdonk FL, Schoustra SE, et al: In-host adaptation and acquired triazole resistance in Aspergillus fumigatus<\/i>: a dilemma for clinical management. Lancet Infect Dis 16: e251–60, 2016.","DOI":"10.1016\/S1473-3099(16)30138-4"},{"key":"31","doi-asserted-by":"crossref","unstructured":"31) Schoustra SE, Debets AJM, Rijs AJMM, Zhang J, Snelders E, Leendertse PC, et al: Environmental hotspots for azole resistance selection of Aspergillus fumigatus<\/i>, the Netherlands. Emerg Infect Dis 25: 1347–53, 2019.","DOI":"10.3201\/eid2507.181625"}],"container-title":["Medical Mycology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/mmj\/66\/3\/66_131\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,6]],"date-time":"2025-09-06T03:33:30Z","timestamp":1757129610000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/mmj\/66\/3\/66_131\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,31]]},"references-count":31,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.3314\/mmj.25-00014","relation":{},"ISSN":["2185-6486","2186-165X"],"issn-type":[{"value":"2185-6486","type":"print"},{"value":"2186-165X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,31]]}}}