{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T17:06:07Z","timestamp":1782320767129,"version":"3.54.5"},"reference-count":43,"publisher":"Aboutscience Srl","license":[{"start":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T00:00:00Z","timestamp":1693267200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["dti"],"abstract":"<jats:p>Background:\u00a0Antimicrobial resistance (AMR) is a global health concern, with extended-spectrum \u03b2-lactamases (ESBLs) and carbapenemases being major contributors. Pleural infection (PI) is a severe condition in West Africa, complicated by AMR. This study aimed to investigate the prevalence and molecular characteristics of ESBL and carbapenemase-producing enterobacteria in pleural effusions in Mali.\nMaterials and methods:\u00a0Pleural fluid samples from 526 patients with pleuritis were analyzed. Enterobacterial species were isolated and identified, and the prevalence of resistance genes (blaOXA-48, blaNDM-1, blaKPC, blaTEM, blaSHV) and virulence factors was determined.\nResults:\u00a0Among the patients, 110 were diagnosed with enterobacterial pleuritis.\u00a0Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis\u00a0were the main pathogens identified. Resistance to \u03b2-lactams and cephalosporins was high, while carbapenems showed good activity. ESBL production was detected in 33.6% of isolates, with\u00a0blaTEM\u00a0being the most common gene. Carbapenemase gene (blaNDM-1) was found in three isolates.\nConclusion:\u00a0The study highlights the high prevalence of multidrug-resistant bacteria and the need for appropriate antibiotic selection based on local resistance patterns. Understanding the molecular characteristics of resistance is crucial for optimizing patient care and developing effective therapeutic strategies. Further research is needed to monitor and control AMR in PIs in Mali.<\/jats:p>","DOI":"10.33393\/dti.2023.2613","type":"journal-article","created":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T09:45:29Z","timestamp":1693302329000},"page":"92-100","source":"Crossref","is-referenced-by-count":7,"title":["ESBL and carbapenemase-producing Enterobacteriaceae in infectious pleural effusions: current epidemiology at H\u00f4pital du Mali"],"prefix":"10.33393","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4325-1929","authenticated-orcid":false,"given":"Aim\u00e9 C\u00e9saire","family":"Kalambry","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5610-5432","authenticated-orcid":false,"given":"Tchamou Malraux Fleury","family":"Potindji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3130-4763","authenticated-orcid":false,"given":"Ibrehima","family":"Guindo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-6058-5982","authenticated-orcid":false,"given":"Ambara","family":"Kassogu\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boubacar Sidiki Ibrahim","family":"Drame","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seydou","family":"Togo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sadio","family":"Yena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seydou","family":"Doumbia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4268-8857","authenticated-orcid":false,"given":"Mahamadou","family":"Diakite","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"18642","published-online":{"date-parts":[[2023,8,29]]},"reference":[{"key":"10105","doi-asserted-by":"crossref","unstructured":"Li P, An J, Wang S, et al. Incidence and prognostic role of pleural effusion in patients with pulmonary embolism: a systematic review and meta-analysis. J Clin Med. 2023:12(6):2315. https:\/\/doi.org\/10.3390\/jcm12062315.","DOI":"10.3390\/jcm12062315"},{"key":"10106","doi-asserted-by":"crossref","unstructured":"Arnold DT, Hamilton FW, Morris TT, et al. Epidemiology of pleural empyema in English hospitals and the impact of influenza. Eur Respir J. 2021;57(6):57. https:\/\/doi.org\/10.1183\/13993003.03546-2020 PMID:33334937","DOI":"10.1183\/13993003.03546-2020"},{"key":"10107","doi-asserted-by":"crossref","unstructured":"Brims F, Popowicz N, Rosenstengel A, et al. Bacteriology and clinical outcomes of patients with culture-positive pleural infection in Western Australia: a 6-year analysis. Respirology. 2019;24(2):171-178. https:\/\/doi.org\/10.1111\/resp.13395 PMID:30187976","DOI":"10.1111\/resp.13395"},{"key":"10108","doi-asserted-by":"crossref","unstructured":"White HD, White BAA, Song J, Fader R, Quiroga P, Arroliga AC. Pleural infections: a 9-year review of bacteriology, case characteristics and mortality. Am J Med Sci. 2013;345(5):349-354. https:\/\/doi.org\/10.1097\/MAJ.0b013e318259bd24 PMID:23044652","DOI":"10.1097\/MAJ.0b013e318259bd24"},{"key":"10109","doi-asserted-by":"crossref","unstructured":"Markatis E, Perlepe G, Afthinos A, et al. Mortality among hospitalized patients with pleural effusions. a multicenter, observational, prospective study. Front Med (Lausanne). 2022;9:828783. https:\/\/doi.org\/10.3389\/fmed.2022.828783 PMID:35280903","DOI":"10.3389\/fmed.2022.828783"},{"key":"10110","doi-asserted-by":"crossref","unstructured":"Tapia MD, Sylla M, Driscoll AJ, et al. The etiology of childhood pneumonia in Mali: findings from the Pneumonia Etiology Research for Child Health (PERCH) study. Pediatr Infect Dis J. 2021;40(9S):S18-S28. https:\/\/doi.org\/10.1097\/INF.0000000000002767 PMID:34448741","DOI":"10.1097\/INF.0000000000002767"},{"key":"10111","doi-asserted-by":"crossref","unstructured":"Singh SR, Teo AKJ, Prem K, et al. Epidemiology of extended-spectrum beta-lactamase and carbapenemase-producing Enterobacterales in the greater mekong subregion: a systematic-review and meta-analysis of risk factors associated with extended-spectrum beta-lactamase and carbapenemase isolation. Front Microbiol. 2021;12:695027. https:\/\/doi.org\/10.3389\/fmicb.2021.695027.","DOI":"10.3389\/fmicb.2021.695027"},{"key":"10112","doi-asserted-by":"crossref","unstructured":"Mustafai MM, Hafeez M, Munawar S, et al. Prevalence of carbapenemase and extended-spectrum \u03b2-lactamase producing Enterobacteriaceae: a cross-sectional study. Antibiotics (Basel). 2023;12(1):12. https:\/\/doi.org\/10.3390\/antibiotics12010148 PMID:36671350","DOI":"10.3390\/antibiotics12010148"},{"key":"10113","doi-asserted-by":"crossref","unstructured":"Sawa T, Kooguchi K, Moriyama K. Molecular diversity of extended-spectrum \u03b2-lactamases and carbapenemases, and antimicrobial resistance. J Intensive Care. 2020;8:13. https:\/\/doi.org\/10.1186\/s40560-020-0429-6.","DOI":"10.1186\/s40560-020-0429-6"},{"key":"10114","doi-asserted-by":"crossref","unstructured":"Castanheira M, Simner PJ, Bradford PA. Extended-spectrum \u03b2-lactamases: an update on their characteristics, epidemiology and detection. JAC Antimicrob Resist. 2021;3(3):dlab092. https:\/\/doi.org\/10.1093\/jacamr\/dlab092.","DOI":"10.1093\/jacamr\/dlab092"},{"key":"10115","unstructured":"World Health Organization. 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Epidemiology and prevalence of extended-spectrum \u03b2-lactamase- and carbapenemase-producing Enterobacteriaceae in humans, animals and the environment in West and Central Africa. Int J Antimicrob Agents. 2021;57(1):106203. https:\/\/doi.org\/10.1016\/j.ijantimicag.2020.106203 PMID:33075511","DOI":"10.1016\/j.ijantimicag.2020.106203"},{"key":"10121","doi-asserted-by":"crossref","unstructured":"Kalambry A, Gaudr\u00e9 N, Drame BS, et al. Profil de r\u00e9sistance aux b\u00eata-lactamines des ent\u00e9robact\u00e9ries isol\u00e9es des pr\u00e9l\u00e8vements urinaires \u00e0 l\u2019H\u00f4pital du Mali. Rev Mali Infectiol Microbiol. 2019;14(2):6-13. https:\/\/doi.org\/10.53597\/remim.v14i2.1363","DOI":"10.53597\/remim.v14i2.1363"},{"key":"10122","doi-asserted-by":"crossref","unstructured":"Muggeo A, Maiga A, Maiga I, et al. First description of IncX3 NDM-5-producing plasmid within Escherichia coli ST448 in Mali. J Med Microbiol. 2020;69(5):685-688. https:\/\/doi.org\/10.1099\/jmm.0.001182 PMID:32375948","DOI":"10.1099\/jmm.0.001182"},{"key":"10123","doi-asserted-by":"crossref","unstructured":"Sangare SA, Rondinaud E, Maataoui N, et al. Very high prevalence of extended-spectrum beta-lactamase-producing Enterobacteriaceae in bacteriemic patients hospitalized in teaching hospitals in Bamako, Mali. PLoS One. 2017;12(2):e0172652. https:\/\/doi.org\/10.1371\/journal.pone.0172652 PMID:28245252","DOI":"10.1371\/journal.pone.0172652"},{"key":"10124","doi-asserted-by":"crossref","unstructured":"Davies HE, Davies RJO, Davies CWH; BTS Pleural Disease Guideline Group. Management of pleural infection in adults: British Thoracic Society pleural disease guideline 2010. Thorax. 2010;65(suppl 2):ii41-ii53. https:\/\/doi.org\/10.1136\/thx.2010.137000 PMID:20696693","DOI":"10.1136\/thx.2010.137000"},{"key":"10125","unstructured":"CASFM\/EUCAST. 2020. CASFM\/EUCAST V1.2 Octobre 2020. 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