{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T14:21:45Z","timestamp":1783952505936,"version":"3.55.0"},"reference-count":42,"publisher":"Aboutscience Srl","issue":"1","license":[{"start":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T00:00:00Z","timestamp":1783900800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["dti"],"abstract":"<jats:p>Introduction: Toxic hepatitis is characterized by enhanced oxidative stress and disruption of antioxidant defensemechanisms in the liver mitochondria. In this study, we investigated the effects of polyphenolic extracts (Helmar-1 and Helmar-2) obtained from Helichrysum maracandicum on mitochondrial antioxidant systems in ratswith experimentally induced toxic hepatitis.Methods: Experimental toxic hepatitis was established in rats through intraperitoneal administration of carbontetrachloride (CCl\u2084) diluted in olive oil (50%, 1 mL\/kg), administered twice weekly over a two-week period. Liverinjury was verified by elevated plasma levels of alanine aminotransferase (ALT) and aspartate aminotransferase(AST). The animals were subsequently treated with Helmar-1 and Helmar-2 extracts at a dose of 20 mg\/kg per dayfor 10 consecutive days. The activities of key antioxidant enzymes (superoxide dismutase, catalase, and glutathioneperoxidase), malondialdehyde (MDA) content, and mitochondrial respiration and oxidative phosphorylationparameters (V\u2082, V\u2083, V\u2084, RCR, and ADP\/O ratio) were assessed in liver mitochondria.Results: Treatment with both Helmar-1 and Helmar-2 extracts resulted in a significant enhancement of antioxidantenzyme activities, a marked reduction in MDA levels, and substantial improvement in mitochondrial respirationand oxidative phosphorylation parameters compared to untreated toxic hepatitis groups.Conclusion: Overall, these results confirm that Helichrysum maracandicum polyphenol extracts improve mitochondrial respiration, oxidative phosphorylation efficiency, antioxidant defense, and membrane stability in toxic hepatitis.<\/jats:p>","DOI":"10.33393\/dti.2026.3805","type":"journal-article","created":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T14:02:40Z","timestamp":1783951360000},"page":"12-21","source":"Crossref","is-referenced-by-count":0,"title":["Comparative effects of Helmar-1 and Helmar-2 polyphenol extracts on mitochondrial bioenergetics, antioxidant defense, and lipid peroxidation in CCl\u2084-induced toxic hepatitis in rats"],"prefix":"10.33393","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5248-245X","authenticated-orcid":false,"given":"Saidahon","family":"Ahmedova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7116-3980","authenticated-orcid":false,"given":"Muzaffar","family":"Asrarov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2212-8692","authenticated-orcid":false,"given":"Sobitjon","family":"Mirzakulov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8881-5973","authenticated-orcid":false,"given":"Aliaksei","family":"Sysa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-2207-5152","authenticated-orcid":false,"given":"Anna","family":"Bokova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2838-4702","authenticated-orcid":false,"given":"Sirojiddin","family":"Sultanov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-9396-8333","authenticated-orcid":false,"given":"Navruzov","family":"Gadoyniyoz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"18642","published-online":{"date-parts":[[2026,7,13]]},"reference":[{"key":"20154","doi-asserted-by":"crossref","unstructured":"Parola M, Robino G. 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