{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T14:38:02Z","timestamp":1740148682051,"version":"3.37.3"},"reference-count":30,"publisher":"Korean Neurosurgical Society","issue":"2","license":[{"start":{"date-parts":[[2022,2,3]],"date-time":"2022-02-03T00:00:00Z","timestamp":1643846400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"crossref","award":["2020YFC1107600"],"award-info":[{"award-number":["2020YFC1107600"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Shandong Province Key R&D Program of China","award":["2019JZZY011106"],"award-info":[{"award-number":["2019JZZY011106"]}]}],"content-domain":{"domain":["jkns.or.kr"],"crossmark-restriction":true},"short-container-title":["J Korean Neurosurg Soc"],"published-print":{"date-parts":[[2022,3,1]]},"abstract":"<jats:p>Objective : To explore the histological feature of the cervical disc degeneration in patients with degenerative ossification (DO) and its potential mechanisms.Methods : A total of 96 surgical segments, from cervical disc degenerative disease patients with surgical treatment, were divided into ossification group (group O, n=46) and non-ossification group (group NO, n=50) based on preoperative radiological exams. Samples of disc tissues and osteophytes were harvested during the decompression operation. The hematoxylin-eosin staining, Masson trichrome staining and Safranin O-fast green staining were used to compare the histological differences between the two groups. And the distribution and content of transforming growth factor (TGF)-\u03b21, p-Smad2 and p-Smad3 between the two groups were compared by a semi-quantitative immunohistochemistry (IHC) method.Results : For all the disc tissues, the content of disc cells and collagen fibers decreased gradually from the outer annulus fibrosus (OAF) to the central nucleus pulposus (NP). Compared with group NO, the number of disc cells in group O increased significantly. But for proteoglycan in the inner annulus fibrosus (IAF) and NP, the content in group O decreased significantly. IHC analysis showed that TGF-\u03b21, p-Smad2, and p-Smad3 were detected in all tissues. For group O, the content of TGF-\u03b21 in the OAF and NP was significantly higher than that in group NO. For p-Smad2 in IAF and p-Smad3 in OAF, the content in group O were significantly higher than group NO.Conclusion : Histologically, cervical disc degeneration in patients with DO is more severe than that without DO. Local higher content of TGF-\u03b21, p-Smad2, and p-Smad3 are involved in the disc degeneration with DO. Further studies with multi-approach analyses are needed to better understand the role of TGF-\u03b2\/Smads signaling pathway in the disc degeneration with DO.<\/jats:p>","DOI":"10.3340\/jkns.2021.0082","type":"journal-article","created":{"date-parts":[[2022,2,3]],"date-time":"2022-02-03T02:14:31Z","timestamp":1643854471000},"page":"186-195","update-policy":"https:\/\/doi.org\/10.3340\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Histological Changes of Cervical Disc Tissue in Patients with Degenerative Ossification"],"prefix":"10.3340","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9977-5866","authenticated-orcid":true,"given":"Yang","family":"Xiong","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8436-6856","authenticated-orcid":true,"given":"Ying-Li","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4469-0720","authenticated-orcid":true,"given":"Yu-Shan","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0930-1593","authenticated-orcid":true,"given":"Xiu-Mei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7917-2205","authenticated-orcid":true,"given":"Xing","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1892","reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1172\/jci118884"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2018.03.019"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/s0940-9602(11)80337-7"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1177\/0363546518773709"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1093\/neuros\/nyw078"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.3714"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2016.08.006"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2017.7464"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1093\/biohorizons\/hzp005"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2011.03.034"},{"key":"ref11","first-page":"543","volume-title":"Animal models for disc degeneration-an update","author":"Jin","year":"2018"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1097\/01.brs.0000068245.27017.9f"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1206791"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1002\/wdev.283"},{"key":"ref15","first-page":"891","volume-title":"Expressions of transforming growth factor \u03b21 and connective tissue growth factor in human lumbar intervertebral discs in different degrees of degeneration","author":"Li","year":"2014"},{"key":"ref16","first-page":"681","volume-title":"TGF-beta signaling in chondrocytes","author":"Li","year":"2005"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.3390\/toxins9110359"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.4062\/biomolther.2019.043"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-10692-5"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1242\/dev.027342"},{"key":"ref21","first-page":"353","volume-title":"Selection of the optimal puncture needle for induction of a rat intervertebral disc degeneration model","author":"Qian","year":"2019"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2013.10.001"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1038\/s41434-018-0004-0"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1007\/s00586-001-0361-y"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1007\/s005860000213"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2013.1348"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1016\/bs.ctdb.2018.11.018"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2016.09.077"},{"key":"ref29","first-page":"897","volume-title":"Association of transforming growth factor-\u03b21 with pathological grading of intervertebral disc degeneration","author":"Yang","year":"2012"},{"key":"ref30","first-page":"2483","volume-title":"Interleukin-1 inhibits Sox9 and collagen type II expression via nuclear factor-kappaB in the cultured human intervertebral disc cells","author":"Yu","year":"2009"}],"container-title":["Journal of Korean Neurosurgical Society"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/jkns.or.kr\/upload\/pdf\/jkns-2021-0082.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/jkns.or.kr\/journal\/view.php?doi=10.3340\/jkns.2021.0082","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/jkns.or.kr\/upload\/pdf\/jkns-2021-0082.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T05:28:58Z","timestamp":1646717338000},"score":1,"resource":{"primary":{"URL":"http:\/\/jkns.or.kr\/journal\/view.php?doi=10.3340\/jkns.2021.0082"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,1]]},"references-count":30,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2022,3]]}},"URL":"https:\/\/doi.org\/10.3340\/jkns.2021.0082","relation":{},"ISSN":["2005-3711","1598-7876"],"issn-type":[{"type":"print","value":"2005-3711"},{"type":"electronic","value":"1598-7876"}],"subject":[],"published":{"date-parts":[[2022,3,1]]},"assertion":[{"value":"2021-04-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-03","order":2,"name":"published","label":"Published online","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-03-01","order":3,"name":"published","label":"Print publication","group":{"name":"publication_history","label":"Publication History"}}]}}