{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T06:33:31Z","timestamp":1771655611268,"version":"3.50.1"},"reference-count":46,"publisher":"Korean Society for Parasitology","issue":"6","license":[{"start":{"date-parts":[[2020,12,29]],"date-time":"2020-12-29T00:00:00Z","timestamp":1609200000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100012421","name":"Agricultural Science and Technology Innovation Program","doi-asserted-by":"publisher","award":["CAAS-ASTIP-2016-LVRI-03"],"award-info":[{"award-number":["CAAS-ASTIP-2016-LVRI-03"]}],"id":[{"id":"10.13039\/501100012421","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Elite Program of Chinese Academy of Agricultural Sciences"}],"content-domain":{"domain":["parasitol.kr"],"crossmark-restriction":true},"short-container-title":["Korean J Parasitol"],"abstract":"<jats:p>&lt;i&gt;Giardia lamblia&lt;\/i&gt; is a common enteric pathogen associated with diarrheal diseases. There are some reports of &lt;i&gt;G. lamblia&lt;\/i&gt; infection among different breeds of cattle in recent years worldwide. However, it is yet to know whether cattle in Jiangxi province, southeastern China is infected with &lt;i&gt;G. lamblia&lt;\/i&gt;. The objectives of the present study were to investigate the prevalence and examine the multilocus genotypes of &lt;i&gt;G. lamblia&lt;\/i&gt; in cattle in Jiangxi province. A total of 556 fecal samples were collected from 3 cattle breeds (dairy cattle, beef cattle, and buffalo) in Jiangxi province, and the prevalence and genotypes of &lt;i&gt;G. lamblia&lt;\/i&gt; were determined by the nested PCR amplification of the beta-giardin (&lt;i&gt;bg&lt;\/i&gt;) gene. A total of 52 samples (9.2%) were positive for &lt;i&gt;G. lamblia&lt;\/i&gt;. The highest prevalence of &lt;i&gt;G. lamblia&lt;\/i&gt; was detected in dairy cattle (20.0%), followed by that in beef cattle (6.4%), and meat buffalo (0.9%). Multilocus sequence typing of &lt;i&gt;G. lamblia&lt;\/i&gt; was performed based on sequences of the &lt;i&gt;bg&lt;\/i&gt;, triose phosphate isomerase and glutamate dehydrogenase loci, and 22, 42, and 52 samples were amplifiable, respectively, forming 15 MLGs. Moreover, one mixed &lt;i&gt;G. lamblia&lt;\/i&gt; infection (assemblages A and E) was found in the present study. Altogether, 6 novel assemblage E subtypes (E41*-E46*) were identified for the first time. These results not only provided baseline data for the control of &lt;i&gt;G. lamblia&lt;\/i&gt; infection in cattle in this southeastern province of China, but also enriched the molecular epidemiological data and genetic diversity of &lt;i&gt;G. lamblia&lt;\/i&gt; in cattle.<\/jats:p>","DOI":"10.3347\/kjp.2020.58.6.681","type":"journal-article","created":{"date-parts":[[2020,12,30]],"date-time":"2020-12-30T08:33:51Z","timestamp":1609317231000},"page":"681-687","update-policy":"https:\/\/doi.org\/10.3347\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Prevalence and Multilocus Genotyping of Giardia lamblia in Cattle in Jiangxi Province, China: Novel Assemblage E Subtypes 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