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A rapid, reliable, and practical immunoassay, named dot immuno-gold filtration assay (DIGFA), was developed to supporting clinical diagnosis of gnathostomiasis. The practical tool detected anti-Gnathostoma-specific IgG4 in human serum using crude extract of third-stage larvae as antigen. The result of the test was shown by anti-human IgG4 monoclonal antibody conjugated colloidal gold. The sensitivity and specificity of the test were both 100% for detection in human sera from patients with gnathostomiasis (13\/13) and from healthy negative controls (50\/50), respectively. Cross-reactivity with heterogonous serum samples from patients with other helminthiases ranged from 0 (trichinosis, paragonimiasis, clonorchiasis, schistosomiasis, and cysticercosis) to 25.0% (sparganosis), with an average of 6.3% (7\/112). Moreover, specific IgG4 antibodies diminished at 6 months after treatment. This study showed that DIGFA for the detection of specific IgG4 in human sera could be a promising tool for the diagnosis of gnathostomiasis and useful for evaluating therapeutic effects.<\/jats:p>","DOI":"10.3347\/kjp.2021.59.3.257","type":"journal-article","created":{"date-parts":[[2021,6,22]],"date-time":"2021-06-22T01:03:46Z","timestamp":1624323826000},"page":"257-263","update-policy":"http:\/\/dx.doi.org\/10.3347\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of Rapid IgG4 Test for Diagnosis of 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