{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T14:37:50Z","timestamp":1740148670017,"version":"3.37.3"},"reference-count":14,"publisher":"Korean Society for Parasitology","issue":"5","license":[{"start":{"date-parts":[[2021,10,22]],"date-time":"2021-10-22T00:00:00Z","timestamp":1634860800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100008005","name":"Yonsei University College of Medicine","doi-asserted-by":"publisher","award":["6-2015-0063"],"award-info":[{"award-number":["6-2015-0063"]}],"id":[{"id":"10.13039\/501100008005","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["parasitol.kr"],"crossmark-restriction":true},"short-container-title":["Korean J Parasitol"],"abstract":"<jats:p>The pathogenic free-living amoeba Naegleria fowleri causes primary amoebic meningoencephalitis, a fatal infection, by penetrating the nasal mucosa and migrating to the brain via the olfactory nerves. N. fowleri can induce host cell death via lytic necrosis. Similar to phosphorylation, O-linked \u03b2-N-acetylglucosamine (O-GlcNAc) glycosylation (O-GlcNAcylation) is involved in various cell-signaling processes, including apoptosis and proliferation, with O-GlcNAc addition and removal regulated by O-GlcNAc transferase and O-GlcNAcase (OGA), respectively. However, the detailed mechanism of host cell death induced by N. fowleri is unknown. In this study, we investigated whether N. fowleri can induce the modulation of O-GlcNAcylated proteins during cell death in Jurkat T cells. Co-incubation with live N. fowleri trophozoites increased DNA fragmentation. In addition, incubation with N. fowleri induced a dramatic reduction in O-GlcNAcylated protein levels in 30 min. Moreover, pretreatment of Jurkat T cells with the OGA inhibitor PUGNAc prevented N. fowleri\u2013induced O-deGlcNAcylation and DNA fragmentation. These results suggest that O-deGlcNAcylation is an important signaling process that occurs during Jurkat T cell death induced by N. fowleri.<\/jats:p>","DOI":"10.3347\/kjp.2021.59.5.501","type":"journal-article","created":{"date-parts":[[2021,10,25]],"date-time":"2021-10-25T04:33:16Z","timestamp":1635136396000},"page":"501-505","update-policy":"https:\/\/doi.org\/10.3347\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Naegleria fowleri Induces Jurkat T Cell Death via O-deGlcNAcylation"],"prefix":"10.3347","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0414-842X","authenticated-orcid":true,"given":"Young Ah","family":"Lee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyeong Ah","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8207-6110","authenticated-orcid":true,"given":"Myeong Heon","family":"Shin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1900","published-online":{"date-parts":[[2021,10,22]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1111\/1440-1681.13192"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.2008.46.4.217"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.12.7.873-876.2005"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2011.01297.x"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/s1367-5931(02)00384-8"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1038\/nature05815"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01025.2008"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1126\/stke.3122005re13"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2018.07.012"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2009.07.018"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.734533"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2008.06.010"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-010-0728-7"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2013.09.011"}],"container-title":["The Korean Journal of Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/parasitol.kr\/upload\/pdf\/kjp-59-5-501.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,7]],"date-time":"2021-11-07T23:12:27Z","timestamp":1636326747000},"score":1,"resource":{"primary":{"URL":"https:\/\/parasitol.kr\/journal\/view.php?doi=10.3347\/kjp.2021.59.5.501"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,22]]},"references-count":14,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2021,10]]}},"URL":"https:\/\/doi.org\/10.3347\/kjp.2021.59.5.501","relation":{},"ISSN":["0023-4001","1738-0006"],"issn-type":[{"type":"print","value":"0023-4001"},{"type":"electronic","value":"1738-0006"}],"subject":[],"published":{"date-parts":[[2021,10,22]]},"assertion":[{"value":"2021-07-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-16","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}