{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T20:45:40Z","timestamp":1792097140658,"version":"4.4.0"},"reference-count":35,"publisher":"Korean Society for Parasitology","issue":"4","license":[{"start":{"date-parts":[[2023,11,28]],"date-time":"2023-11-28T00:00:00Z","timestamp":1701129600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"funder":[{"name":"PAICyT-UANL","award":["CN943-19"],"award-info":[{"award-number":["CN943-19"]}]},{"name":"PAICyT-UANL","award":["125-CS-2022"],"award-info":[{"award-number":["125-CS-2022"]}]},{"name":"CONAHCyT"}],"content-domain":{"domain":["parahostdis.org"],"crossmark-restriction":true},"short-container-title":["Parasites Hosts Dis"],"abstract":"<jats:p>Chagas disease, caused by Trypanosoma cruzi parasite, is a significant but neglected tropical public health issue in Latin America due to the diversity of its genotypes and pathogenic profiles. This complexity is compounded by the adverse effects of current treatments, underscoring the need for new therapeutic options that employ medicinal plant extracts without negative side effects. Our research aimed to evaluate the trypanocidal activity of Bidens pilosa fractions against epimastigote and trypomastigote stages of T. cruzi, specifically targeting the Brener and Nuevo Le\u00f3n strains\u2014the latter isolated from Triatoma gerstaeckeri in General Ter\u00e1n, Nuevo Le\u00f3n, M\u00e9xico. We processed the plant\u2019s aerial parts (stems, leaves, and flowers) to obtain a methanolic extract (Bp-mOH) and fractions with varying solvent polarities. These preparations inhibited more than 90% of growth at concentrations as low as 800 \u03bcg\/ml for both parasite stages. The median lethal concentration (LC50) values for the Bp-mOH extract and its fractions were below 500 \u03bcg\/ml. Tests for cytotoxicity using Artemia salina and Vero cells and hemolytic activity assays for the extract and its fractions yielded negative results. The methanol fraction (BPFC3MOH1) exhibited superior inhibitory activity. Its functional groups, identified as phenols, enols, alkaloids, carbohydrates, and proteins, include compounds such as 2-hydroxy-3-methylbenzaldehyde (50.9%), pentadecyl prop-2-enoate (22.1%), and linalool (15.4%). Eight compounds were identified, with a match confirmed by the National Institute of Standards and Technology (NIST-MS) software through mass spectrometry analysis.<\/jats:p>","DOI":"10.3347\/phd.23069","type":"journal-article","created":{"date-parts":[[2023,11,30]],"date-time":"2023-11-30T06:48:52Z","timestamp":1701326932000},"page":"405-417","update-policy":"https:\/\/doi.org\/10.3347\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["In vitro anti-Trypanosoma cruzi activity of methanolic extract of Bidens pilosa and identification of active compounds by gas chromatography-mass spectrometry analysis"],"prefix":"10.3347","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4493-2830","authenticated-orcid":true,"given":"Gabriel Enrique","family":"C\u00e1zares-Jaramillo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zinnia Judith","family":"Molina-Garza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Itza Eloisa","family":"Luna-Cruz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luisa Yolanda","family":"Sol\u00eds-Soto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Luis","family":"Rosales-Encina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8220-6314","authenticated-orcid":true,"given":"Lucio","family":"Galaviz-Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1900","published-online":{"date-parts":[[2023,11,28]]},"reference":[{"key":"ref1","volume-title":""},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1021\/cr500546h"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1055\/s-0029-1185377"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.3109\/13880200903485729"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.11648\/j.ajlm.20170204.15"},{"key":"ref6","first-page":"99","volume-title":"Evaluation of yerba mate fruit pulp total and fractioned soxhlet extraction using thermal analyses","author":"daSilveira","year":"2019"},{"key":"ref7","first-page":"21","volume-title":"Isolation, purification, and identification of curcuminoids from turmeric (Curcuma longa L.) by column chromatography","author":"Revathy","year":"2011"},{"key":"ref8","volume-title":"Manual de pr\u00e1cticas de bioqu\u00edmica [por]"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2019.02.008"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(85)90073-8"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1007\/pl00008532"},{"key":"ref12","first-page":"29","volume-title":"","author":"Sherma","year":"2003"},{"key":"ref13","volume-title":"","author":"Kramida"},{"key":"ref14","first-page":"1","volume-title":"Crown gall tumors on potato discs and brine shrimp lethality: two simple bioassays for higher plant screening and fractionation. 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