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Helminth parasites secrete calreticulin (CRT), which regulates or blocks the host\u2019s immune response. However, no data on &lt;i&gt;A. ceylanicum&lt;\/i&gt; calreticulin (&lt;i&gt;Ace&lt;\/i&gt;-CRT) are available. We investigated the biological function of recombinant &lt;i&gt;Ace&lt;\/i&gt;-CRT (&lt;i&gt;rAce&lt;\/i&gt;-CRT). rAce-CRT showed reliable antigenicity and stimulated the proliferation of mouse splenocytes and canine peripheral blood mononuclear cells. Quantitative reverse-transcription PCR assays revealed that r&lt;i&gt;Ace&lt;\/i&gt;-CRT primarily promoted the expression of T helper 2 cytokines, particularly IL-13, in canine peripheral blood lymphocytes. r&lt;i&gt;Ace&lt;\/i&gt;-CRT inhibited complement-mediated sheep erythrocyte hemolysis in vitro. 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