{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T12:55:41Z","timestamp":1791377741174,"version":"4.3.2"},"reference-count":20,"publisher":"Korean Society for Parasitology","issue":"2","license":[{"start":{"date-parts":[[2024,5,27]],"date-time":"2024-05-27T00:00:00Z","timestamp":1716768000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["RS-2023-00248480"],"award-info":[{"award-number":["RS-2023-00248480"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["parahostdis.org"],"crossmark-restriction":true},"short-container-title":["Parasites Hosts Dis"],"abstract":"<jats:p>We investigated organ specific &lt;i&gt;Toxocara canis&lt;\/i&gt; larval migration in mice infected with &lt;i&gt;T. canis&lt;\/i&gt; larvae. We observed the worm burden and systemic immune responses. Three groups of BALB\/c mice (&lt;i&gt;n&lt;\/i&gt;=5 each) were orally administered 1,000 &lt;i&gt;T. canis&lt;\/i&gt; 2nd stage larvae to induce larva migrans. Mice were sacrificed at 1, 3, and 5 weeks post-infection. Liver, lung, brain, and eye tissues were collected. Tissue from 2 mice per group was digested for larval count, while the remaining 3 mice underwent histological analysis. Blood hematology and serology were evaluated and compared to that in a control uninfected group (&lt;i&gt;n&lt;\/i&gt;=5) to assess the immune response. Cytokine levels in bronchoalveolar lavage (BAL) fluid were also analyzed. We found that, 1 week post-infection, the mean parasite load in the liver (72\u00b17.1), brain (31\u00b14.2), lungs (20\u00b15.7), and eyes (2\u00b10) peaked and stayed constant until the 3 weeks. By 5-week post-infection, the worm burden in the liver and lungs significantly decreased to 10\u00b14.2 and 9\u00b15.7, respectively, while they remained relatively stable in the brain and eyes (18\u00b14.2 and 1\u00b10, respectively). Interestingly, ocular larvae resided in all retinal layers, without notable inflammation in outer retina. Mice infected with &lt;i&gt;T. canis&lt;\/i&gt; exhibited elevated levels of neutrophils, monocytes, eosinophils, and immunoglobulin E. At 5 weeks post-infection, interleukin (IL)-5 and IL-13 levels were elevated in BAL fluid. Whereas IL-4, IL-10, IL-17, and interferon-\u03b3 levels in BAL fluid were similar to that in controls. Our findings demonstrate that a small portion of &lt;i&gt;T. canis&lt;\/i&gt; larvae migrate to the eyes and brain within the first week of infection. Minimal tissue inflammation was observed, probably due to increase of anti-inflammatory cytokines. This study contributes to our understanding of the histological and immunological responses to &lt;i&gt;T. canis&lt;\/i&gt; infection in mice, which may have implications to further understand human toxocariasis.<\/jats:p>","DOI":"10.3347\/phd.23125","type":"journal-article","created":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T05:41:47Z","timestamp":1717134107000},"page":"243-250","update-policy":"https:\/\/doi.org\/10.3347\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Organ-specific Toxocara canis larvae migration and host immune response in experimentally infected mice"],"prefix":"10.3347","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7092-5429","authenticated-orcid":true,"given":"Min Seok","family":"Kim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6118-2069","authenticated-orcid":true,"given":"Yan","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3692-7169","authenticated-orcid":true,"given":"Se Joon","family":"Woo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1900","published-online":{"date-parts":[[2024,5,27]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.5415\/apallergy.2014.4.3.134"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-010-2145-y"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1542\/peds.9.1.7"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1097\/OPX.0000000000000404"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2012.12.033"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1079\/JOH200175"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-015-0962-7"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-011-2371-y"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2007.11.001"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrs.2012.12.033"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.3341\/kjo.2016.30.4.258"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1001\/jama.194.13.1345"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002938"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2014.10.004"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016\/s0304-4017(03)00046-3"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2010.03.006"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2006.06.002"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2022.869976"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2005.02250.x"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016\/s0192-0561(00)00013-8"}],"container-title":["Parasites, Hosts and Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/parahostdis.org\/upload\/pdf\/PHD-23125.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,3]],"date-time":"2024-06-03T02:13:04Z","timestamp":1717380784000},"score":1,"resource":{"primary":{"URL":"https:\/\/parahostdis.org\/journal\/view.php?doi=10.3347\/PHD.23125"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,27]]},"references-count":20,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2024,5]]}},"URL":"https:\/\/doi.org\/10.3347\/phd.23125","relation":{},"ISSN":["2982-5164","2982-6799"],"issn-type":[{"value":"2982-5164","type":"print"},{"value":"2982-6799","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,27]]},"assertion":[{"value":"2023-12-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}