{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,29]],"date-time":"2025-08-29T00:02:22Z","timestamp":1756425742132,"version":"3.44.0"},"reference-count":16,"publisher":"Korean Society for Parasitology","issue":"3","license":[{"start":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T00:00:00Z","timestamp":1755648000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["RS-2024-00456300"],"award-info":[{"award-number":["RS-2024-00456300"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003710","name":"Korea Health Industry Development Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003625","name":"Ministry of Health and Welfare","doi-asserted-by":"publisher","award":["RS-2023-KH139971"],"award-info":[{"award-number":["RS-2023-KH139971"]}],"id":[{"id":"10.13039\/501100003625","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["parahostdis.org"],"crossmark-restriction":true},"short-container-title":["Parasites Hosts Dis"],"abstract":"<jats:p>Trichomonads are flagellated protozoa that have occasionally been detected in the human respiratory tract, although detection rates have often been underestimated. We applied a nested PCR assay targeting the 18S rRNA gene of trichomonads to induced sputum from asthma patients to determine the prevalence of &lt;i&gt;Trichomonas&lt;\/i&gt;. Induced sputum was collected from 41 adults with asthma and analyzed through nested PCR using broad-range trichomonad primers and DNA sequencing for species identification. Nested PCR detected trichomonad DNA in 10 of the 41 (24.4%) samples. Sequencing and phylogenetic analysis revealed &lt;i&gt;Trichomonas tenax&lt;\/i&gt; in 8 cases and &lt;i&gt;Tetratrichomonas&lt;\/i&gt; sp. in 2 cases. These findings indicate that trichomonads can be present in the lower airways of patients with asthma, warranting further investigation into their clinical relevance.<\/jats:p>","DOI":"10.3347\/phd.25028","type":"journal-article","created":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T06:21:48Z","timestamp":1756362108000},"page":"254-257","update-policy":"https:\/\/doi.org\/10.3347\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Detection of trichomonads in induced sputum from asthma patients in Korea"],"prefix":"10.3347","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9537-5726","authenticated-orcid":true,"given":"Myung-hee","family":"Yi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9308-4917","authenticated-orcid":true,"given":"Myungjun","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7416-3377","authenticated-orcid":true,"given":"Jun Ho","family":"Choi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0731-8439","authenticated-orcid":true,"given":"Yoon Hee","family":"Cho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-0258-8100","authenticated-orcid":true,"given":"Hyun Kyung","family":"Oh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0249-8749","authenticated-orcid":true,"given":"Jung-Won","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2456-6298","authenticated-orcid":true,"given":"Ju Yeong","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1900","published-online":{"date-parts":[[2025,8,20]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-019-4118-9"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1093\/trstmh\/try027"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.3347\/PHD.22160"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.3347\/PHD.24049"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-20-1-1"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01056-13"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016\/s0755-4982(05)84193-4"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00353-09"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci11050203"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2011.07.036"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajem.2019.03.029"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1017\/s0031182005008000"},{"key":"ref13","first-page":"33","volume-title":"Prevalence of oral trichomoniasis in patients with periodontitis and gingivitis using PCR and direct smear","author":"Athari","year":"2007"},{"key":"ref14","first-page":"197","volume-title":"Pulmonary trichomoniasis: improved diagnosis by using polymerase chain reaction targeting <italic>Trichomonas tenax<\/italic> 18S rRNA gene in sputum specimens","author":"Mahmoud","year":"2004"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.8.3886-3887.2004"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/2023010"}],"container-title":["Parasites, Hosts and Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/parahostdis.org\/upload\/pdf\/PHD-25028.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T06:21:59Z","timestamp":1756362119000},"score":1,"resource":{"primary":{"URL":"https:\/\/parahostdis.org\/journal\/view.php?doi=10.3347\/PHD.25028"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,20]]},"references-count":16,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,8]]}},"URL":"https:\/\/doi.org\/10.3347\/phd.25028","relation":{},"ISSN":["2982-5164","2982-6799"],"issn-type":[{"value":"2982-5164","type":"print"},{"value":"2982-6799","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,20]]},"assertion":[{"value":"2025-04-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}