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Mast cells play a key role in the inflammatory response against T. vaginalis infection. In this study, we examined the signaling pathways involved in mast cell activation induced by T. vaginalis-derived secretory products (TvSP), focusing on IKK2, calcium, MAP kinase (MAPK), and PI3 kinase (PI3K). TvSP stimulation induced phosphorylation and degradation of I\u03baB, indicating NF-\u03baB activation, and triggered phosphorylation of ERK1\/2, p38 MAPK, and AKT. TvSP also increased the surface expression of CD63, a marker of exocytosis, which was reduced by IKK inhibition, calcium chelation, or blockade of PI3K and PKC. Furthermore, inhibition of PI3K or MAPKs decreased TvSP-induced interleukin-8 production. These results suggest that IKK2 and calcium are critical for TvSP-induced degranulation, while PI3K and MAPK pathways contribute to interleukin-8 production in mast cells.<\/jats:p>","DOI":"10.3347\/phd.25079","type":"journal-article","created":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T06:39:34Z","timestamp":1768199974000},"page":"45-51","update-policy":"https:\/\/doi.org\/10.3347\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["IKK2, calcium, MAP kinase, and PI3 kinase are required for exocytosis and interleukin-8 production in human mast cells stimulated by Trichomonas vaginalis-derived secretory products"],"prefix":"10.3347","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4661-4190","authenticated-orcid":true,"given":"Shin Hye","family":"Park","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0414-842X","authenticated-orcid":true,"given":"Young 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