{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T07:03:20Z","timestamp":1785135800067,"version":"3.55.0"},"reference-count":28,"publisher":"Korean Society for Parasitology","issue":"3","license":[{"start":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T00:00:00Z","timestamp":1782086400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["parahostdis.org"],"crossmark-restriction":true},"short-container-title":["PHD"],"abstract":"<jats:p>Profilins are ubiquitous pan-allergens responsible for cross-reactivity between pollens and plant foods. While we previously demonstrated that recombinant Acanthamoeba profilin (rAc-PF) drives allergic airway inflammation via Th2\/Th17 pathways in murine models, its clinical relevance in human allergic disease remains unclear. This study investigated rAc-PF as a novel inhalant allergen and its immunological impact on patients with allergic airway diseases. A total of 176 patients with allergic airway diseases underwent skin prick tests with rAc-PF and a standard panel of 55 common aeroallergens. Acanthamoeba-specific and rAc-PF\u2013specific serum IgE levels were quantified using ELISA. To elucidate immune mechanisms, peripheral blood mononuclear cells from atopic asthma patients were stimulated with rAc-PF, and Th2\/Th17 cytokine production was analyzed. Thirteen patients (7.4%) showed positive skin prick tests reactions to rAc-PF. This sensitization was significantly associated with tree, grass, and weed pollens, indicating a pan-allergen characteristic due to high cross-reactivity. Notably, one patient sensitized to rAc-PF reacted to no other common inhalants, suggesting rAc-PF as a unique causative agent. Patients exhibited significantly elevated levels of serum Acanthamoeba-specific and rAc-PF\u2013specific IgE compared to healthy controls. Furthermore, rAc-PF stimulation of peripheral blood mononuclear cells from asthmatic patients induced robust production of IL-4, IL-5, IL-13, and IL-17A in humans. rAc-PF is identified as a novel allergen capable of inducing IgE-mediated sensitization and mixed Th2\/Th17 responses. The strong association with pollen sensitization supports its role as an environmental pan-allergen. Therefore, rAc-PF should be considered a clinically relevant diagnostic target, especially in patients with polysensitization or unidentified triggers.<\/jats:p>","DOI":"10.3347\/phd.26021","type":"journal-article","created":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T00:52:52Z","timestamp":1782089572000},"page":"229-236","update-policy":"https:\/\/doi.org\/10.3347\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Acanthamoeba profilin as a novel airway allergen with diverse sensitization patterns including pollen cross-reactivity"],"prefix":"10.3347","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4992-1866","authenticated-orcid":true,"given":"Mi-Kyung","family":"Park","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4065-2962","authenticated-orcid":true,"given":"Hye-Kyung","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6696-7981","authenticated-orcid":true,"given":"Hak Sun","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1900","published-online":{"date-parts":[[2026,6,22]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2020.00191"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2008.02971.x"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3049"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1186\/1939-4551-7-12"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1038\/nri3786"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.02.024"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1038\/nature07204"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.anai.2023.11.014"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.3347\/PHD.25050"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1007\/s12551-020-00723-3"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1126\/science.1857985"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1515\/BC.2004.089"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1159\/000236263"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006979"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0092726"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0011085"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.151.3.7881653"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.2147\/JIR.S450866"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1996.tb00056.x"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.2000.00821.x"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.165563"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016\/s0091-6749(96)70197-5"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1126\/science.283.5405.1183"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1172\/JCI17748"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2012.76"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2001.117929"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.6.2593"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1016\/s1053-2498(02)01234-2"}],"container-title":["Parasites, Hosts and Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/parahostdis.org\/upload\/pdf\/PHD-26021.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T06:36:32Z","timestamp":1785134192000},"score":1,"resource":{"primary":{"URL":"https:\/\/parahostdis.org\/journal\/view.php?doi=10.3347\/PHD.26021"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,22]]},"references-count":28,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,7]]}},"URL":"https:\/\/doi.org\/10.3347\/phd.26021","relation":{},"ISSN":["2982-5164","2982-6799"],"issn-type":[{"value":"2982-5164","type":"print"},{"value":"2982-6799","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,22]]},"assertion":[{"value":"2026-03-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}