{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:14:05Z","timestamp":1791936845998,"version":"4.3.4"},"reference-count":51,"publisher":"Inter-Research Science Center","license":[{"start":{"date-parts":[[2020,10,8]],"date-time":"2020-10-08T00:00:00Z","timestamp":1602115200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.int-res.com\/journals\/terms-of-use\/"}],"content-domain":{"domain":["int-res.com"],"crossmark-restriction":false},"short-container-title":["Dis. Aquat. Org."],"published-print":{"date-parts":[[2020,10,8]]},"abstract":"<jats:p>Gastritis and gastric ulcers are well-recognized symptoms in cetaceans, and the genus <jats:italic>Helicobacter<\/jats:italic> is considered as the main cause. In this study, we examined the gastric fluid of captive common bottlenose dolphins <jats:italic>Tursiops truncatus<\/jats:italic> with gastric diseases in order to isolate the organisms responsible for diagnosis and treatment. Four Gram-negative, rod-shaped isolates (TSB<jats:sup>T<\/jats:sup>, TSH1, TSZ, and TSH3) with tightly coiled spirals with 2-4 turns and 2-6 bipolar, sheathed flagella, were obtained from gastric fluids of common bottlenose dolphins with gastric diseases. Phylogenetic analysis, based on 16S rRNA, <jats:italic>atp<\/jats:italic>A, and 60 kDa heat-shock protein (<jats:italic>hsp<\/jats:italic>60) genes, demonstrated that these isolates form a novel lineage within the genus <jats:italic>Helicobacter<\/jats:italic>. Analyses of 16S rRNA, <jats:italic>atp<\/jats:italic>A, and <jats:italic>hsp<\/jats:italic>60 gene sequences showed that isolate TSB<jats:sup>T<\/jats:sup> was most closely related to <jats:italic>H. cetorum<\/jats:italic> MIT99-5656<jats:sup>T<\/jats:sup> (98.5% similarity), <jats:italic>H. pylori<\/jats:italic> ATCC 43504<jats:sup>T<\/jats:sup> (76.7% similarity), and <jats:italic>H. pylori<\/jats:italic> ATCC 43504<jats:sup>T<\/jats:sup> (78.0% similarity), respectively. Type strains of <jats:italic>Helicobacter<\/jats:italic> showing resistance to 2% NaCl have not been reported previously; however, these novel isolates were resistant to 2% NaCl. Culture supernatant of some isolates induced intracellular vacuolization in mammalian cultured cells. These data, together with the different morphological and biochemical characteristics of the isolates, reveal that these isolates represent a novel species for which we propose the name <jats:italic>Helicobacter delphinicola<\/jats:italic> sp. nov. with type strain TSB<jats:sup>T<\/jats:sup> (= JCM 32789<jats:sup>T<\/jats:sup> = TSD-183<jats:sup>T<\/jats:sup>). Future studies will confirm whether <jats:italic>H. delphinicola<\/jats:italic> plays a role in lesion etiopathogenesis in cetaceans.<\/jats:p>","DOI":"10.3354\/dao03511","type":"journal-article","created":{"date-parts":[[2020,8,4]],"date-time":"2020-08-04T10:10:41Z","timestamp":1596535841000},"page":"157-169","update-policy":"https:\/\/doi.org\/10.3354\/policy","source":"Crossref","is-referenced-by-count":14,"title":["Helicobacter delphinicola sp. nov., isolated from common bottlenose dolphins Tursiops truncatus with gastric diseases"],"prefix":"10.3354","volume":"141","author":[{"given":"T","family":"Segawa","sequence":"first","affiliation":[{"name":"Cetacean Research Center, Graduate School of Bioresources, Mie University, 1577 Kurimamachiya, Tsu, Mie 514-8507, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Ohno","sequence":"additional","affiliation":[{"name":"Port of Nagoya Public Aquarium, 1-3 Minato-machi, Minato-ku, Nagoya 455-0033, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Tsuchida","sequence":"additional","affiliation":[{"name":"Academy of Emerging Sciences, Chubu University, Kasugai, Aichi 487-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Ushida","sequence":"additional","affiliation":[{"name":"Academy of Emerging Sciences, Chubu University, Kasugai, Aichi 487-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Yoshioka","sequence":"additional","affiliation":[{"name":"Cetacean Research Center, Graduate School of Bioresources, Mie University, 1577 Kurimamachiya, Tsu, Mie 514-8507, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1913","reference":[{"key":"Ref1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms10516"},{"key":"Ref2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-41-1-148"},{"key":"Ref3","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.057141-0"},{"key":"Ref4","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.59.4.1264-1270.1991"},{"key":"Ref5","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.2.356-365.2004"},{"key":"Ref6","doi-asserted-by":"publisher","DOI":"10.7589\/2013-02-047"},{"key":"Ref7","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(12)70137-7"},{"key":"Ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2018.04.014"},{"key":"Ref9","doi-asserted-by":"publisher","DOI":"10.1578\/AM.41.3.2015.345"},{"key":"Ref10","doi-asserted-by":"publisher","DOI":"10.3390\/toxins8060173"},{"key":"Ref11","unstructured":"Frances MDG, Leslie AD, Karyl LW (2018) Bacterial infections and diseases. 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