{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T03:27:12Z","timestamp":1789788432669,"version":"4.0.1"},"reference-count":31,"publisher":"Inter-Research Science Center","license":[{"start":{"date-parts":[[2020,11,5]],"date-time":"2020-11-05T00:00:00Z","timestamp":1604534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.int-res.com\/journals\/terms-of-use\/"}],"content-domain":{"domain":["int-res.com"],"crossmark-restriction":false},"short-container-title":["Dis. Aquat. Org."],"published-print":{"date-parts":[[2020,11,5]]},"abstract":"<jats:p><jats:italic>Nocardia seriolae<\/jats:italic> is the causative agent of nocardiosis in both marine and freshwater fish. Here, we report on multiple outbreaks of nocardiosis associated with elevated mortality (23-35%) in farmed largemouth bass in Sichuan, China, from 2017 to 2018. A total of 9 strains isolated from diseased largemouth bass were identified as <jats:italic>N. seriolae<\/jats:italic> by phenotypic characterization, 16S rRNA and <jats:italic>hsp65<\/jats:italic> gene sequence analysis. The clinical signs of infected largemouth bass included hemorrhage, skin ulcers and prominent tubercles varying in size in the gill, liver, spleen and kidney. Experimental infection indicated that these isolates were the pathogens responsible for the mortalities. <jats:italic>In vitro<\/jats:italic> antibacterial activities of 12 antibiotics against <jats:italic>N. seriolae<\/jats:italic> isolates were determined as minimum inhibitory concentrations. Histopathological observation of diseased fish infected with <jats:italic>N. seriolae<\/jats:italic> showed necrotizing granulomatous hepatitis, nephritis, splenitis, epithelial hypertrophy and hyperplasia with degenerative changes of the epithelium in the gill. Large quantities of bacterial aggregates were found in the necrotic area of the granuloma by Lillie-Twort Gram stain and immunocytochemistry. Our findings indicated that <jats:italic>N. seriolae<\/jats:italic> is a serious threat to the largemouth bass <jats:italic>Micropterus salmoides<\/jats:italic> industry in Southwest China.<\/jats:p>","DOI":"10.3354\/dao03517","type":"journal-article","created":{"date-parts":[[2020,8,4]],"date-time":"2020-08-04T10:10:41Z","timestamp":1596535841000},"page":"13-21","update-policy":"https:\/\/doi.org\/10.3354\/policy","source":"Crossref","is-referenced-by-count":60,"title":["Nocardia seriolae: a serious threat to the largemouth bass Micropterus salmoides industry in Southwest China"],"prefix":"10.3354","volume":"142","author":[{"given":"X","family":"Lei","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan 611130, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan 611130, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Geng","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan 611130, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan 611130, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"PO","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan 611130, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Aquaculture, Sichuan Agricultural University, Wenjiang Sichuan 611130, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Aquaculture, Sichuan Agricultural University, Wenjiang Sichuan 611130, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z","family":"Zuo","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan 611130, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"He","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan 611130, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan 611130, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan 611130, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan 611130, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W","family":"Lai","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan 611130, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1913","reference":[{"key":"Ref1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12134"},{"key":"Ref2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2761.2000.00217.x"},{"key":"Ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2007.06.004"},{"key":"Ref4","unstructured":"CLSI (Clinical and Laboratory Standards Institue) (2015) Performance standards for antimicrobial susceptibility testing, 25rd informational supplement, M100-S25. 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