{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T02:23:53Z","timestamp":1788143033579,"version":"build-2803163510"},"reference-count":75,"publisher":"Inter-Research Science Center","license":[{"start":{"date-parts":[[2020,12,17]],"date-time":"2020-12-17T00:00:00Z","timestamp":1608163200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.int-res.com\/journals\/terms-of-use\/"}],"content-domain":{"domain":["int-res.com"],"crossmark-restriction":false},"short-container-title":["Dis. Aquat. Org."],"published-print":{"date-parts":[[2020,12,17]]},"abstract":"<jats:p><jats:italic>Brucella<\/jats:italic> spp. and <jats:italic>Leptospira<\/jats:italic> spp. antibodies were surveyed in 35 southern elephant seals (SESs) <jats:italic>Mirounga leonina<\/jats:italic> at Elephant Island (South Shetland Islands), western Antarctic peninsula, in the Austral summer of 2003 and 2004. The rose Bengal test and a commercial competitive ELISA (c-ELISA) were used to detect <jats:italic>Brucella<\/jats:italic> spp. exposure, and the microscopic agglutination test (MAT) with 22 live serovars was used to determine anti-<jats:italic>Leptospira<\/jats:italic> spp. antibodies. We found evidence of <jats:italic>Brucella<\/jats:italic> spp. exposure in 3 of 35 (8.6%) SESs tested via the c-ELISA displaying high percentage inhibition (PI), similar to other studies in pinnipeds in which <jats:italic>Brucella<\/jats:italic> spp. antibodies have been determined. Two of the 3 positives were pups (PI = 70.4 and 86.6%), while the third was an adult female (PI = 48.8%). The 3 c-ELISA positive SESs were additionally tested via the serum agglutination test but were found to be negative. All individuals were negative for antibodies against 22 <jats:italic>Leptospira<\/jats:italic> spp. serovars by MAT. These results contribute to the knowledge and monitoring of zoonotic pathogens with epizootic potential in Southern Ocean pinnipeds. Given the potential impact that pathogens may have on the abundance of wild (sometimes threatened and endangered) populations, constant monitoring and surveillance are required to prevent pathogen spread, particularly under forecast climate change scenarios.<\/jats:p>","DOI":"10.3354\/dao03548","type":"journal-article","created":{"date-parts":[[2020,10,15]],"date-time":"2020-10-15T10:35:14Z","timestamp":1602758114000},"page":"161-170","update-policy":"https:\/\/doi.org\/10.3354\/policy","source":"Crossref","is-referenced-by-count":7,"title":["Serological screening for Brucella spp. and Leptospira spp. antibodies in southern elephant seals Mirounga leonina from Elephant Island, Antarctica, in 2003 and 2004"],"prefix":"10.3354","volume":"142","author":[{"given":"AM","family":"S\u00e1nchez-Sarmiento","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Patologia Comparada de Animais Selvagens, Departamento de Patologia, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, SP 05508-270, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V","family":"Ruoppolo","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologia Comparada de Animais Selvagens, Departamento de Patologia, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, SP 05508-270, Brazil"},{"name":"International Fund for Animal Welfare (IFAW), Yarmouth Port, MA 02675, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MMC","family":"Muelbert","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo (IMar\/UNIFESP), Rua Dr Carvalho de Mendon\u00e7a 144, Santos, SP 11070-100, Brazil"},{"name":"Institute for Marine and Antarctic Studies, University of Tasmania, Battery Point, Tasmania 7004, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JS","family":"Ferreira Neto","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Zoonoses Bacterianas, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, SP 05508-270, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JL","family":"Cat\u00e3o-Dias","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologia Comparada de Animais Selvagens, Departamento de Patologia, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, SP 05508-270, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1913","reference":[{"key":"Ref1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.164.8.250"},{"key":"Ref2","unstructured":"Alton CG, Jones IM, Angus RD, Verger JM (1988) Techniques for the brucellosis laboratory. 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