{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T16:18:56Z","timestamp":1746807536907,"version":"3.40.5"},"reference-count":15,"publisher":"Inter-Research Science Center","license":[{"start":{"date-parts":[[2021,1,28]],"date-time":"2021-01-28T00:00:00Z","timestamp":1611792000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.int-res.com\/journals\/terms-of-use\/"}],"content-domain":{"domain":["int-res.com"],"crossmark-restriction":false},"short-container-title":["Dis. Aquat. Org."],"published-print":{"date-parts":[[2021,1,28]]},"abstract":"<jats:p>Neoplasia is rarely reported in decapod crustaceans, and sarcoma has not been previously reported in any crab species. A California king crab <jats:italic>Paralithodes californiensis<\/jats:italic> with a recent history of autotomy (4 legs lost) and anorexia was found dead. Grossly, the crab had a pigmented ulcer on the right cheliped merus. Necropsy tissue samples were placed in 10% neutral buffered formalin and processed routinely for histology. Both histochemical (i.e. Brown and Brenn Gram, Fite-Faraco acid fast, Fontana-Masson, Giemsa, hematoxylin and eosin, Masson\u2019s trichrome, periodic acid-Schiff [PAS], phosphotungstic acid-hematoxylin, and von Kossa) and immunohistochemical (i.e. cytokeratin, vimentin, and lysozyme) stains were performed. The body wall (presumably of the right cheliped merus) was ulcerated and subtended by a densely cellular, unencapsulated, invasive neoplasm composed of spindle cells arranged in intersecting streams and bundles embedded in a small to moderate amount of fibromatous stroma. Neoplastic cells were oval to elongate with fibrillar, pale eosinophilic cytoplasm that occasionally contained moderate numbers of small, spherical, brightly eosinophilic granules that were highlighted with PAS and Giemsa stains. Neoplastic cells had mild atypia and no evident mitoses. Immunohistochemical stains were noncontributory. This neoplasm is consistent with hemocytic sarcoma of semi-granulocytic origin. Decapod crustaceans have 3 types of hemocytes: hyalinocytes, granulocytes, and semi-granulocytes. Neoplastic cells had PAS- and Giemsa-positive granules, which are present in both semi-granulocytes and granulocytes. Semi-granulocytes can elongate and are associated with deposition of extracellular matrix during some immune responses. Neoplastic cells were elongate and associated with deposition of matrix. These findings suggest neoplastic cells were of semi-granulocytic origin.<\/jats:p>","DOI":"10.3354\/dao03551","type":"journal-article","created":{"date-parts":[[2020,10,28]],"date-time":"2020-10-28T10:23:03Z","timestamp":1603880583000},"page":"13-18","update-policy":"https:\/\/doi.org\/10.3354\/policy","source":"Crossref","is-referenced-by-count":1,"title":["Hemocytic sarcoma of the body wall in a California king crab Paralithodes californiensis"],"prefix":"10.3354","volume":"143","author":[{"given":"EEB","family":"LaDouceur","sequence":"first","affiliation":[{"name":"Joint Pathology Center, 606 Stephen Sitter Ave., Silver Spring, MD 20910, USA"},{"name":"Northwest ZooPath, 654 W Main St., Monroe, WA 98272, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JL","family":"Gray","sequence":"additional","affiliation":[{"name":"Joint Pathology Center, 606 Stephen Sitter Ave., Silver Spring, MD 20910, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Smolowitz","sequence":"additional","affiliation":[{"name":"Roger Williams University, 1 Old Ferry Rd., Bristol, RI 02809, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Schleiderer","sequence":"additional","affiliation":[{"name":"Monterey Bay Aquarium, 886 Cannery Row, Monterey, CA 93940, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Murray","sequence":"additional","affiliation":[{"name":"Monterey Bay Aquarium, 886 Cannery Row, Monterey, CA 93940, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1913","reference":[{"unstructured":"Brock JA, Lightner DV (1990) Diseases of crustacea, Chap 3. In: Kinne O (ed) Diseases of marine animals, Vol 3. Biologische Anstalt Helgoland, Hamburg, p 245-424","key":"Ref1"},{"unstructured":"Cerenius L, Jiravanichpaisal P, Liu H, Soderhall I (2010) Crustacean immunity, Chap 13. In: Soderhall K (ed) Invertebrate immunity. Springer, New York, NY, p 239-259","key":"Ref2"},{"doi-asserted-by":"crossref","unstructured":"Donaldson WE (2005) Biological field techniques for lithodid crabs. Alaska Sea Grant College Program, Fairbanks, AK","key":"Ref3","DOI":"10.4027\/bftlc.2005"},{"doi-asserted-by":"publisher","key":"Ref4","DOI":"10.1016\/S0044-8486(00)00418-X"},{"doi-asserted-by":"publisher","key":"Ref5","DOI":"10.1016\/0022-2011(87)90159-5"},{"doi-asserted-by":"publisher","key":"Ref6","DOI":"10.3354\/dao003101"},{"doi-asserted-by":"publisher","key":"Ref7","DOI":"10.4081\/ejh.2010.e9"},{"doi-asserted-by":"publisher","key":"Ref8","DOI":"10.1016\/j.cvex.2016.07.001"},{"doi-asserted-by":"publisher","key":"Ref9","DOI":"10.18353\/crustacea.Special2006.6_167"},{"doi-asserted-by":"publisher","key":"Ref10","DOI":"10.1016\/0022-2011(78)90012-5"},{"doi-asserted-by":"publisher","key":"Ref11","DOI":"10.1016\/j.jip.2013.08.013"},{"doi-asserted-by":"publisher","key":"Ref12","DOI":"10.1016\/0022-2011(73)90135-3"},{"doi-asserted-by":"publisher","key":"Ref13","DOI":"10.1016\/0022-2011(87)90144-3"},{"doi-asserted-by":"publisher","key":"Ref14","DOI":"10.1177\/1753425909102876"},{"doi-asserted-by":"publisher","key":"Ref15","DOI":"10.1002\/ijc.23947"}],"container-title":["Diseases of Aquatic Organisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.int-res.com\/articles\/dao2021\/143\/d143p013.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.int-res.com\/articles\/dao2021\/143\/d143p013.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,28]],"date-time":"2021-01-28T13:03:25Z","timestamp":1611839005000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.int-res.com\/abstracts\/dao\/v143\/p13-18\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,28]]},"references-count":15,"URL":"https:\/\/doi.org\/10.3354\/dao03551","relation":{},"ISSN":["0177-5103","1616-1580"],"issn-type":[{"type":"print","value":"0177-5103"},{"type":"electronic","value":"1616-1580"}],"subject":[],"published":{"date-parts":[[2021,1,28]]}}}