{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T06:22:50Z","timestamp":1775197370803,"version":"3.50.1"},"reference-count":22,"publisher":"Inter-Research Science Center","license":[{"start":{"date-parts":[[2021,2,25]],"date-time":"2021-02-25T00:00:00Z","timestamp":1614211200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.int-res.com\/journals\/terms-of-use\/"}],"content-domain":{"domain":["int-res.com"],"crossmark-restriction":false},"short-container-title":["Dis. Aquat. Org."],"published-print":{"date-parts":[[2021,2,25]]},"abstract":"<jats:p>A pathogen was isolated from diseased pink-tailed chalceus <jats:italic>Chalceus macrolepidotus<\/jats:italic> during a high-mortality outbreak in a freshwater culture farm in Liaoyang, China. The diseased fish were characterized by disoriented behaviors, exophthalmos, and redness and swelling of the top of the head. A Gram-negative, pure strain of bacteria (CM0428) was isolated from the brain, kidney, and liver. The isolate was identified as <jats:italic>Vibrio cholerae<\/jats:italic> based on <jats:italic>ompW<\/jats:italic> gene amplification and 16S rRNA and <jats:italic>gyrB<\/jats:italic> gene sequence analysis. Serogroup testing indicated that CM0428 was a non-O1\/O139 strain of <jats:italic>V. cholerae<\/jats:italic>. The challenge test showed that CM0428 exhibited strong virulence to pink-tailed chalceus, and <jats:italic>hlyA<\/jats:italic> and <jats:italic>toxR<\/jats:italic> virulence-related genes were detected. The isolate was sensitive to multi-class antibiotics, but resistant to tetracycline. Histological examination revealed that <jats:italic>V. cholerae<\/jats:italic> CM0428 infection caused multiple organ and tissue lesions, and typical pathological features were cell degeneration and necrosis.<\/jats:p>","DOI":"10.3354\/dao03565","type":"journal-article","created":{"date-parts":[[2020,11,25]],"date-time":"2020-11-25T10:56:25Z","timestamp":1606301785000},"page":"139-145","update-policy":"https:\/\/doi.org\/10.3354\/policy","source":"Crossref","is-referenced-by-count":5,"title":["First case of non-O1\/O139 Vibrio cholerae infection in the pink-tailed chalceus Chalceus macrolepidotus in China"],"prefix":"10.3354","volume":"143","author":[{"given":"P","family":"Ni","sequence":"first","affiliation":[{"name":"Dalian Key Laboratory of Marine Animal Disease Control and Prevention, Dalian Ocean University, Liaoning 116023, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y","family":"Guo","sequence":"additional","affiliation":[{"name":"Dalian Key Laboratory of Marine Animal Disease Control and Prevention, Dalian Ocean University, Liaoning 116023, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Liu","sequence":"additional","affiliation":[{"name":"Dalian Key Laboratory of Marine Animal Disease Control and Prevention, Dalian Ocean University, Liaoning 116023, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Ma","sequence":"additional","affiliation":[{"name":"Dalian Key Laboratory of Marine Animal Disease Control and Prevention, Dalian Ocean University, Liaoning 116023, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Guo","sequence":"additional","affiliation":[{"name":"Dalian Key Laboratory of Marine Animal Disease Control and Prevention, Dalian Ocean University, Liaoning 116023, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X","family":"Zhao","sequence":"additional","affiliation":[{"name":"Dalian Key Laboratory of Marine Animal Disease Control and Prevention, Dalian Ocean University, Liaoning 116023, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Ye","sequence":"additional","affiliation":[{"name":"Dalian Key Laboratory of Marine Animal Disease Control and Prevention, Dalian Ocean University, Liaoning 116023, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1913","reference":[{"key":"Ref1","doi-asserted-by":"publisher","DOI":"10.1038\/35083691"},{"key":"Ref2","unstructured":"CLSI (Clinical and Laboratory Standards Institute) (2016) M45: Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria, 3rd edn. 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