{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T05:12:10Z","timestamp":1785388330280,"version":"3.55.0"},"reference-count":44,"publisher":"Inter-Research Science Center","license":[{"start":{"date-parts":[[2021,5,27]],"date-time":"2021-05-27T00:00:00Z","timestamp":1622073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.int-res.com\/journals\/terms-of-use\/"}],"content-domain":{"domain":["int-res.com"],"crossmark-restriction":false},"short-container-title":["Dis. Aquat. Org."],"published-print":{"date-parts":[[2021,5,27]]},"abstract":"<jats:p>In this study, a Gram-negative bacterium was isolated from diseased<jats:italic>Scylla paramamosain<\/jats:italic>and tentatively named strain QX17. The bacterial isolate was identified as<jats:italic>Vibrio parahaemolyticus<\/jats:italic>based on morphological and biochemical characteristics and molecular identification with the 16S rRNA and HSP60 genes. In the challenge experiment,<jats:italic>S. paramamosain<\/jats:italic>injected intramuscularly with the<jats:italic>V. parahaemolyticus<\/jats:italic>isolate developed pathological signs similar to the naturally diseased mud crabs. The infection experiment also showed that the median lethal dosage (LD<jats:sub>50<\/jats:sub>) for QX17 was 4.79 \u00d7 10<jats:sup>2<\/jats:sup>CFU g<jats:sup>-1<\/jats:sup>(crab weight). Histopathological analysis of the diseased mud crabs infected with<jats:italic>V. parahaemolyticus<\/jats:italic>showed deformation and basement membrane rupture of hepatopancreatic tubules in the hepatopancreas, and disordered and broken muscle fiber in the muscle. Antimicrobial susceptibility tests revealed that QX17 was highly sensitive to most of the tested aminoglycosides, tetracyclines, and quinolones. To the best of our knowledge, this is the first study reporting isolation and antibiotic sensitivities of<jats:italic>V. parahaemolyticus<\/jats:italic>from cultured mud crabs. The discovery of<jats:italic>V. parahaemolyticus<\/jats:italic>in cultured mud crabs not only adds to the growing list of emerging pathogens in crab aquaculture in China, but also highlights the necessity of developing early detection strategies and appropriate interventions to reduce the damage caused by<jats:italic>V. parahaemolyticus<\/jats:italic>in mud crab aquaculture.<\/jats:p>","DOI":"10.3354\/dao03587","type":"journal-article","created":{"date-parts":[[2021,3,2]],"date-time":"2021-03-02T12:06:24Z","timestamp":1614686784000},"page":"187-196","update-policy":"https:\/\/doi.org\/10.3354\/policy","source":"Crossref","is-referenced-by-count":13,"title":["Outbreak of vibriosis associated with Vibrio parahaemolyticus in the mud crab Scylla paramamosain cultured in China"],"prefix":"10.3354","volume":"144","author":[{"given":"J","family":"Xie","sequence":"first","affiliation":[{"name":"School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province 315832, PR China"},{"name":"Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo, Zhejiang Province 315832, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Mei","sequence":"additional","affiliation":[{"name":"School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province 315832, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Jin","sequence":"additional","affiliation":[{"name":"School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province 315832, PR China"},{"name":"Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo, Zhejiang Province 315832, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Bu","sequence":"additional","affiliation":[{"name":"School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province 315832, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province 315832, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province 315832, PR China"},{"name":"Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo, Zhejiang Province 315832, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Q","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province 315832, PR China"},{"name":"Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo, Zhejiang Province 315832, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Ma","sequence":"additional","affiliation":[{"name":"School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province 315832, PR China"},{"name":"Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo, Zhejiang Province 315832, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province 315832, PR China"},{"name":"Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo, Zhejiang Province 315832, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1913","reference":[{"key":"Ref1","doi-asserted-by":"publisher","DOI":"10.1016\/S0044-8486(97)00045-8"},{"key":"Ref2","doi-asserted-by":"publisher","DOI":"10.3354\/dao035077"},{"key":"Ref3","unstructured":"Anjay, Das SC, Kumar A, Kaushik P, Kurmi B (2014) Occurrence of Vibrio parahaemolyticus in marine fish and shellfish. 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