{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T07:16:16Z","timestamp":1790752576100,"version":"4.1.0"},"reference-count":26,"publisher":"Inter-Research Science Center","license":[{"start":{"date-parts":[[2022,8,18]],"date-time":"2022-08-18T00:00:00Z","timestamp":1660780800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["int-res.com"],"crossmark-restriction":false},"short-container-title":["Dis. Aquat. Org."],"published-print":{"date-parts":[[2022,8,18]]},"abstract":"<jats:p>Conservation of endangered animal species is a major task of zoos. Husbandry and breeding of Atlantic puffins <jats:italic>Fratercula arctica<\/jats:italic> in captivity is challenging. In 2019, the entire chick population (n = 4 chicks) in Berne Animal Park\u2019s Atlantic puffin colony (Bern, Switzerland) died within 7 d. Due to supply constraints, the chicks had been fed with wild-caught European minnows <jats:italic>Phoxinus phoxinus<\/jats:italic>. At necropsy, the main pathological finding in all deceased puffin chicks was a multifocal, moderate to severe subacute heterophilic and granulomatous enteritis with intralesional adult trematodes and eggs. Metacercariae surrounded by few necrotic cells and scattered macrophages were found in the brain and spinal cord of the food fish. Additional microbiological analyses of both the puffin chicks and fish were unremarkable. <jats:italic>Diplostomum phoxini<\/jats:italic> DNA could be identified in formalin-fixed paraffin-embedded tissue from the small intestine of all puffin chicks and European minnows following PCR and sequencing of the 18S ribosomal RNA gene and the internal transcribed spacer (ITS1) region. This report illustrates the importance of intensive health checks of food fish for animal species kept in captivity.<\/jats:p>","DOI":"10.3354\/dao03683","type":"journal-article","created":{"date-parts":[[2022,7,12]],"date-time":"2022-07-12T09:10:16Z","timestamp":1657617016000},"page":"161-167","update-policy":"https:\/\/doi.org\/10.3354\/policy","source":"Crossref","is-referenced-by-count":3,"title":["Fatal Diplostomum phoxini infection in captive Atlantic puffin Fratercula arctica chicks following ingestion of infected European minnows Phoxinus phoxinus"],"prefix":"10.3354","volume":"150","author":[{"given":"S","family":"Hoby","sequence":"first","affiliation":[{"name":"Berne Animal Park, Tierparkweg 1, 3005 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I","family":"Berenguer Veiga","sequence":"additional","affiliation":[{"name":"Institute of Animal Pathology, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Olias","sequence":"additional","affiliation":[{"name":"Institute of Animal Pathology, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"K\u00fcchler","sequence":"additional","affiliation":[{"name":"Institute of Animal Pathology, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Sch\u00f6nb\u00e4chler","sequence":"additional","affiliation":[{"name":"Berne Animal Park, Tierparkweg 1, 3005 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Blatter","sequence":"additional","affiliation":[{"name":"Berne Animal Park, Tierparkweg 1, 3005 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Schmidt-Posthaus","sequence":"additional","affiliation":[{"name":"Institute for Fish and Wildlife Health, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1913","reference":[{"key":"Ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2014.04.009"},{"key":"Ref2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1977.tb15813.x"},{"key":"Ref3","doi-asserted-by":"publisher","DOI":"10.1126\/science.1200674"},{"key":"Ref4","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2109"},{"key":"Ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2012.10.019"},{"key":"Ref6","doi-asserted-by":"publisher","DOI":"10.3389\/fpsyg.2019.01746"},{"key":"Ref7","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-10-356"},{"key":"Ref8","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-24.3.407"},{"key":"Ref9","doi-asserted-by":"publisher","DOI":"10.2307\/3286550"},{"key":"Ref10","doi-asserted-by":"publisher","DOI":"10.1002\/9780813804620.ch12"},{"key":"Ref11","unstructured":"IUCN (2013) Guidelines for reintroductions and other conservation translocations. 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