{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T21:56:57Z","timestamp":1790027817831,"version":"4.0.1"},"reference-count":33,"publisher":"Inter-Research Science Center","license":[{"start":{"date-parts":[[2023,8,3]],"date-time":"2023-08-03T00:00:00Z","timestamp":1691020800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["int-res.com"],"crossmark-restriction":false},"short-container-title":["Dis. Aquat. Org."],"published-print":{"date-parts":[[2023,8,3]]},"abstract":"<jats:p>The lack of internationally harmonised criteria for interpreting the data generated by standardised susceptibility testing methods presents a serious obstacle for the development of prudent use of antimicrobials in aquaculture. The data required to set epidemiological cut-off values for minimum inhibitory concentrations for antibiotic agents against <jats:italic>Vibrio harveyi<\/jats:italic> was determined using a standard microdilution method that specified the use of cation-adjusted Mueller Hinton broth and incubation at 28\u00b0C for 24 to 28 h. In total, 120 observations were made in 4 independent laboratories from 109 unique isolates. The aggregated data from these laboratories were analysed by the normalised resistance method and by ECOFFinder to calculate epidemiological cut-off values. The data for chloramphenicol, meropenem and sulfamethoxazole were not considered as suitable for analysis. The data for ampicillin indicated that this species is innately resistant to this agent. No acceptable ranges for quality control strains have been set for ceftazidime and, therefore, only provisional cut-off values could be generated for this agent. The epidemiological cut-off values were, however, calculated for the other 6 agents. These values were \u22640.5 \u00b5g ml<jats:sup>-1<\/jats:sup> for enrofloxacin, \u22641 \u00b5g ml<jats:sup>-1<\/jats:sup> for florfenicol, oxolinic acid and oxytetracycline, \u22644 \u00b5g ml<jats:sup>-1<\/jats:sup> for gentamicin and \u22640.5\/9.5 \u00b5g ml<jats:sup>-1<\/jats:sup> for trimethoprim\/sulfamethoxazole. Evidence is presented demonstrating that the data for these 6 antimicrobial agents was of sufficient quantity and quality that they could be used by the relevant authorities to set internationally harmonised, consensus epidemiological cut-off values for <jats:italic>V. harveyi<\/jats:italic>.<\/jats:p>","DOI":"10.3354\/dao03740","type":"journal-article","created":{"date-parts":[[2023,6,15]],"date-time":"2023-06-15T10:33:10Z","timestamp":1686825190000},"page":"35-42","update-policy":"https:\/\/doi.org\/10.3354\/policy","source":"Crossref","is-referenced-by-count":7,"title":["Setting epidemiological cut-off values for Vibrio harveyi relevant to MIC data generated by a standardised microdilution method"],"prefix":"10.3354","volume":"155","author":[{"given":"P","family":"Smith","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Galway, Galway H91 TK33, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Cortinovis","sequence":"additional","affiliation":[{"name":"Fish Pathology Unit, Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Pretto","sequence":"additional","affiliation":[{"name":"Fish Pathology Unit, Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Manfrin","sequence":"additional","affiliation":[{"name":"Fish Pathology Unit, Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Florio","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medical Sciences, Alma Mater Studiorum Universit\u00e0 di Bologna, 40064 Ozzano Emilia (BO), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Fioravanti","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medical Sciences, Alma Mater Studiorum Universit\u00e0 di Bologna, 40064 Ozzano Emilia (BO), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Baron","sequence":"additional","affiliation":[{"name":"Anses, Ploufragan-Plouzan\u00e9-Niort Laboratory, Mycoplasmology-Bacteriology and Antimicrobial Resistance Unit, 22440 Ploufragan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Le Devendec","sequence":"additional","affiliation":[{"name":"Anses, Ploufragan-Plouzan\u00e9-Niort Laboratory, Mycoplasmology-Bacteriology and Antimicrobial Resistance Unit, 22440 Ploufragan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Jouy","sequence":"additional","affiliation":[{"name":"Anses, Ploufragan-Plouzan\u00e9-Niort Laboratory, Mycoplasmology-Bacteriology and Antimicrobial Resistance Unit, 22440 Ploufragan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Le Breton","sequence":"additional","affiliation":[{"name":"Vet\u2019eau-Selarl du Dr Alain Le Breton, 1989 Rue des Pyr\u00e9n\u00e9es, 31330 Grenade sur Garonne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Picon-Camacho","sequence":"additional","affiliation":[{"name":"Vet\u2019eau-Selarl du Dr Alain Le Breton, 1989 Rue des Pyr\u00e9n\u00e9es, 31330 Grenade sur Garonne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"IG","family":"Zupi\u010di\u0107","sequence":"additional","affiliation":[{"name":"Laboratory for Fish and Molluscs Diseases, Croatian Veterinary Institute, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Orai\u0107","sequence":"additional","affiliation":[{"name":"Laboratory for Fish and Molluscs Diseases, Croatian Veterinary Institute, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Zrn\u010di\u0107","sequence":"additional","affiliation":[{"name":"Laboratory for Fish and Molluscs Diseases, Croatian Veterinary Institute, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1913","reference":[{"key":"Ref1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines8040734"},{"key":"Ref2","doi-asserted-by":"crossref","unstructured":"Amaro C, Fouz B, Sanjuan E, Romalde JL (2020) Vibriosis. 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