{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T21:57:17Z","timestamp":1790027837637,"version":"4.0.1"},"reference-count":34,"publisher":"Inter-Research Science Center","license":[{"start":{"date-parts":[[2023,8,31]],"date-time":"2023-08-31T00:00:00Z","timestamp":1693440000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["int-res.com"],"crossmark-restriction":false},"short-container-title":["Dis. Aquat. Org."],"published-print":{"date-parts":[[2023,8,31]]},"abstract":"<jats:p>This work aims to generate the data needed to set epidemiological cut-off values for minimum inhibitory concentration (MIC) and disc-diffusion zone measurements of <jats:italic>Vibrio anguillarum<\/jats:italic>. A total of 261 unique isolates were tested, applying standard methods specifying incubation at 28\u00b0C for 24-28 h. Aggregated MIC distributions for a total of 247 isolates were determined in 9 laboratories for 11 agents. Data aggregations of the disc zone for the 10 agents analysed contained between 157 and 218 observations made by 4 to 7 laboratories. Acceptable ranges for quality control (QC) reference strains were available for 7 agents and the related multi-laboratory aggregated data were censored, excluding the data of a laboratory that failed to meet QC requirements. Statistical methods were applied to calculate epidemiological cut-off values. Cut-off values for MIC data were calculated for florfenicol (\u22641 \u00b5g ml<jats:sup>-1<\/jats:sup>), gentamicin (\u22644 \u00b5g ml<jats:sup>-1<\/jats:sup>), oxytetracycline (\u22640.25 \u00b5g ml<jats:sup>-1<\/jats:sup>) and trimethoprim\/sulfamethoxazole (\u22640.125\/2.38 \u00b5g ml<jats:sup>-1<\/jats:sup>). The cut-off values for disc zone data were calculated for enrofloxacin (\u226529 mm), florfenicol (\u226527 mm), gentamicin (\u226519 mm), oxolinic acid (\u226524 mm), oxytetracycline (\u226524 mm) and trimethoprim\/sulfamethoxazole (\u226526 mm). MIC and disc-diffusion zone data for the other agents where not supported by QC, thus yielding only provisional cut-off values (meropenem, ceftazidime). Regardless of whether QC is available, some of the aggregated MIC distributions (enrofloxacin, oxolinic acid), disc zone (sulfamethoxazole), and MIC and disc-diffusion distributions (ampicillin, chloramphenicol) did not meet the statistical requirements. The data produced will be submitted to the Clinical Laboratory Standards Institute for their consideration in setting international consensus epidemiological cut-off values.<\/jats:p>","DOI":"10.3354\/dao03745","type":"journal-article","created":{"date-parts":[[2023,7,6]],"date-time":"2023-07-06T08:21:00Z","timestamp":1688631660000},"page":"109-123","update-policy":"https:\/\/doi.org\/10.3354\/policy","source":"Crossref","is-referenced-by-count":12,"title":["Epidemiological cut-off values for Vibrio anguillarum MIC and disc diffusion data generated by standardised methods"],"prefix":"10.3354","volume":"155","author":[{"given":"P","family":"Smith","sequence":"first","affiliation":[{"name":"School of Natural Science, University of Galway, Galway H91 TK33, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Le Devendec","sequence":"additional","affiliation":[{"name":"Anses, Ploufragan-Plouzan\u00e9-Niort Laboratory, 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Selarl du Dr Alain Le Breton, 1989 Rue des Pyr\u00e9n\u00e9es, 31330 Grenade sur Garonne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Zrn\u010di\u0107","sequence":"additional","affiliation":[{"name":"Laboratory for Fish and Molluscs Diseases, Croatian Veterinary Institute, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"IG","family":"Zupi\u010di\u0107","sequence":"additional","affiliation":[{"name":"Istanbul University, Faculty of Aquatic Sciences, 34234 Istanbul, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Orai\u0107","sequence":"additional","affiliation":[{"name":"Laboratory for Fish and Molluscs Diseases, Croatian Veterinary Institute, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Karata\u015f","sequence":"additional","affiliation":[{"name":"Istanbul University, Faculty of Aquatic Sciences, 34234 Istanbul, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Verner-Jeffreys","sequence":"additional","affiliation":[{"name":"Centre for Environment, Fisheries and Aquaculture Science, Weymouth DT4 8UB, UK"},{"name":"UK FAO Reference Centre for AMR, Weymouth DT4 8UB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"AW","family":"Joseph","sequence":"additional","affiliation":[{"name":"Centre for Environment, Fisheries and Aquaculture Science, Weymouth DT4 8UB, UK"},{"name":"UK FAO Reference Centre for AMR, Weymouth DT4 8UB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Light","sequence":"additional","affiliation":[{"name":"Veterinary Medicines Directorate (VMD), Woodham Lane, New Haw, Surrey KT15 3LS, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"van Essen-Zandbergen","sequence":"additional","affiliation":[{"name":"Wageningen Bioveterinary Research, 8221 RA Lelystad, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B","family":"van Gelderen","sequence":"additional","affiliation":[{"name":"Wageningen Bioveterinary Research, 8221 RA Lelystad, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Voorbergen-Laarman","sequence":"additional","affiliation":[{"name":"Wageningen Bioveterinary Research, 8221 RA Lelystad, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"OLM","family":"Haenen","sequence":"additional","affiliation":[{"name":"Wageningen Bioveterinary Research, 8221 RA Lelystad, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"KT","family":"Veldman","sequence":"additional","affiliation":[{"name":"Wageningen Bioveterinary Research, 8221 RA Lelystad, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Madsen","sequence":"additional","affiliation":[{"name":"Section for Fish and Shellfish Diseases, National Institute of Aquatic Resources (DTU Aqua), Technical University of Denmark, 2800 Kongens Lyngby, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"KK","family":"Mouritsen","sequence":"additional","affiliation":[{"name":"Section for Fish and Shellfish Diseases, National Institute of Aquatic Resources (DTU Aqua), Technical University of Denmark, 2800 Kongens Lyngby, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Smith Svanevik","sequence":"additional","affiliation":[{"name":"Department of Contaminants and Biohazards, Institute of Marine Research, 5005 Bergen, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F","family":"H\u00e5konsholm","sequence":"additional","affiliation":[{"name":"Department of Contaminants and Biohazards, Institute of Marine Research, 5005 Bergen, 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World Organisation for Animal Health. www.oie.int\/en\/international-standard-setting\/aquatic-code\/access-online\/"}],"container-title":["Diseases of Aquatic Organisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.int-res.com\/articles\/dao_oa\/d155p109.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.int-res.com\/articles\/dao_oa\/d155p109.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T05:13:31Z","timestamp":1745558011000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.int-res.com\/abstracts\/dao\/v155\/p109-123\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,31]]},"references-count":34,"URL":"https:\/\/doi.org\/10.3354\/dao03745","relation":{},"ISSN":["0177-5103","1616-1580"],"issn-type":[{"value":"0177-5103","type":"print"},{"value":"1616-1580","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,31]]},"assertion":[{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}