{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T23:40:02Z","timestamp":1785368402215,"version":"3.55.0"},"reference-count":32,"publisher":"Inter-Research Science Center","license":[{"start":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T00:00:00Z","timestamp":1702512000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["int-res.com"],"crossmark-restriction":false},"short-container-title":["Dis. Aquat. Org."],"published-print":{"date-parts":[[2023,12,14]]},"abstract":"<jats:p>This work generates the data needed to set epidemiological cut-off values for disc-diffusion zone measurements of <jats:italic>Vibrio cholerae<\/jats:italic>. The susceptibility of 147 European isolates of non-O1\/non-O139 <jats:italic>V. cholerae<\/jats:italic> to 19 antibiotics was established using a standardised disc diffusion method which specified incubation of Mueller Hinton agar plates at 35\u00b0C. Epidemiological cut-off values were calculated by analysis of the zone size data with the statistically based normalised resistance interpretation method. Cut-off values for 17 agents were calculated by analysis of the aggregated data from all 4 laboratories participating in this study. The cut-off values calculated were \u226518 mm for amoxicillin\/clavulanate, \u226518 mm for amikacin, \u226519 mm for ampicillin, \u226527 mm for cefepime, \u226531 mm for cefotaxime, \u226524 mm for ceftazidime, \u226524 mm for chloramphenicol, \u226531 mm for ciprofloxacin, \u226516 mm for erythromycin, \u2265 27 mm for florfenicol, \u226516 mm for gentamicin, \u226523 mm for imipenem, \u226525 mm for meropenem, \u226529 mm for nalidixic acid, \u226528 mm for norfloxacin, \u226513 mm for streptomycin and \u226523 mm for tetracycline. For the other 2 agents the data from 1 laboratory was excluded from the censored aggregation because the data from that laboratory was considered excessively imprecise. The cut-off values for these 2 agents calculated for the aggregation of the data from 3 laboratories were \u226523 mm for trimethoprim and \u226524 mm for trimethoprim\/sulfamethoxazole. These zone size data will be submitted to the Clinical Laboratory Standards Institute (CLSI) and European Committee for Antimicrobial Susceptibility Testing (EUCAST) for their consideration in setting international consensus epidemiological cut-off values for non O1\/non-O139 <jats:italic>V. cholerae<\/jats:italic>.<\/jats:p>","DOI":"10.3354\/dao03766","type":"journal-article","created":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T10:31:31Z","timestamp":1698143491000},"page":"115-121","update-policy":"https:\/\/doi.org\/10.3354\/policy","source":"Crossref","is-referenced-by-count":3,"title":["Epidemiological cut-off values for non-O1\/ non-O139 Vibrio cholerae disc diffusion data generated by standardised methods"],"prefix":"10.3354","volume":"156","author":[{"given":"P","family":"Smith","sequence":"first","affiliation":[{"name":"School of Natural Science, University of Galway, Galway H91 TK33, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Le Devendec","sequence":"additional","affiliation":[{"name":"Anses, Ploufragan-Plouzan\u00e9-Niort Laboratory, Mycoplasmology-Bacteriology and Antimicrobial Resistance Unit, 22440 Ploufragan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Jouy","sequence":"additional","affiliation":[{"name":"Anses, Ploufragan-Plouzan\u00e9-Niort Laboratory, Mycoplasmology-Bacteriology and Antimicrobial Resistance Unit, 22440 Ploufragan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Larvor","sequence":"additional","affiliation":[{"name":"Anses, Ploufragan-Plouzan\u00e9-Niort Laboratory, Mycoplasmology-Bacteriology and Antimicrobial Resistance Unit, 22440 Ploufragan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Lesne","sequence":"additional","affiliation":[{"name":"\u00c9cole des Hautes \u00c9tudes en Sante Publique, Laboratoire d\u2019\u00c9tude et de Recherche en Environnent et Sante, 35000 Rennes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"AKT","family":"Kirschner","sequence":"additional","affiliation":[{"name":"Institute for Hygiene and Applied Immunology - Water Microbiology, Medical University Vienna, Kinderspitalgasse 15, 1090 Austria"},{"name":"Division Water Quality & Health, Karl Landsteiner University of Health Sciences, Dr. Karl-Dorrek-Stra\u00dfe 30, 3500 Krems, Austria"},{"name":"Interuniversity Cooperation Centre Water & Health, www.waterandhealth.at"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Rehm","sequence":"additional","affiliation":[{"name":"Institute for Hygiene and Applied Immunology - Water Microbiology, Medical University Vienna, Kinderspitalgasse 15, 1090 Austria"},{"name":"Division Water Quality & Health, Karl Landsteiner University of Health Sciences, Dr. Karl-Dorrek-Stra\u00dfe 30, 3500 Krems, Austria"},{"name":"Interuniversity Cooperation Centre Water & Health, www.waterandhealth.at"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Leopold","sequence":"additional","affiliation":[{"name":"Division Water Quality & Health, Karl Landsteiner University of Health Sciences, Dr. Karl-Dorrek-Stra\u00dfe 30, 3500 Krems, Austria"},{"name":"Interuniversity Cooperation Centre Water & Health, www.waterandhealth.at"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Pleininger","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology and Hygiene, Austrian Agency for Health and Food Safety, Austria, Waehringer Strasse 25A, 1094 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F","family":"Heger","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology and Hygiene, Austrian Agency for Health and Food Safety, Austria, Waehringer Strasse 25A, 1094 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"J\u00e4ckel","sequence":"additional","affiliation":[{"name":"Consultant Laboratory for Vibrio spp. in Food, Department Biological Safety, German Federal Institute for Risk Assessment, Max-Dohrn Str. 8-10, 1089 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"G\u00f6llner","sequence":"additional","affiliation":[{"name":"Consultant Laboratory for Vibrio spp. in Food, Department Biological Safety, German Federal Institute for Risk Assessment, Max-Dohrn Str. 8-10, 1089 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Nekat","sequence":"additional","affiliation":[{"name":"Consultant Laboratory for Vibrio spp. in Food, Department Biological Safety, German Federal Institute for Risk Assessment, Max-Dohrn Str. 8-10, 1089 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JA","family":"Hammerl","sequence":"additional","affiliation":[{"name":"Consultant Laboratory for Vibrio spp. in Food, Department Biological Safety, German Federal Institute for Risk Assessment, Max-Dohrn Str. 8-10, 1089 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Baron","sequence":"additional","affiliation":[{"name":"Anses, Ploufragan-Plouzan\u00e9-Niort Laboratory, Mycoplasmology-Bacteriology and Antimicrobial Resistance Unit, 22440 Ploufragan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1913","reference":[{"key":"Ref1","unstructured":"Baron S, Chevalier S, Lesne J (2007) Vibrio cholerae in the environment: a simple method for reliable identification of the species. J Health Popul Nutr 25:312-318"},{"key":"Ref2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01671"},{"key":"Ref3","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01637"},{"key":"Ref4","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.01179"},{"key":"Ref5","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03540-14"},{"key":"Ref6","unstructured":"CLSI (2010) Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria, CLSI guideline M45, 2nd edn. Clinical and Laboratory Standards Institute, Wayne, PA"},{"key":"Ref7","unstructured":"CLSI (2010) Performance standards for antimicrobial susceptibility testing, twentieth informational supplement. M100-S20. Clinical and Laboratory Standards Institute, Wayne, PA"},{"key":"Ref8","unstructured":"CLSI (2015) Methods for antimicrobial dilution and disc susceptibility testing of infrequently isolated of fastidious bacteria. CLSI guideline M45, 3rd edn. Clinical and Laboratory Standards Institute, Wayne, PA"},{"key":"Ref9","unstructured":"CLSI (2015) Performance standards for antimicrobial susceptibility testing, twenty-fifth informational supplement M100-S25. Clinical and Laboratory Standards Institute, Wayne, PA"},{"key":"Ref10","unstructured":"CLSI (2016) Performance standards for antimicrobial susceptibility testing, twenty-sixth informational supplement M100-S26. Clinical and Laboratory Standards Institute, Wayne, PA"},{"key":"Ref11","unstructured":"CLSI (2018) Development of in vitro susceptibility testing criteria and quality control parameters. CLSI guideline M23, 5th edn. Clinical and Laboratory Standards Institute, Wayne, PA"},{"key":"Ref12","unstructured":"CLSI (2018) Performance standards for antimicrobial susceptibility testing, twenty-eighth informational supplement M100-S28. Clinical and Laboratory Standards Institute, Wayne, PA"},{"key":"Ref13","unstructured":"CLSI (2020) Performance standards for antimicrobial susceptibility testing of bacteria isolated from aquatic animals. CLSI guideline VET04, 3rd edn. Clinical Laboratory Standards Institute, Wayne, PA"},{"key":"Ref14","unstructured":"CLSI (2021) Performance standards for antimicrobial susceptibility testing. Thirty-first informational supplement M100-S31. Clinical and Laboratory Standards Institute, Wayne, PA"},{"key":"Ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2019.06.031"},{"key":"Ref16","doi-asserted-by":"publisher","DOI":"10.2436\/20.1501.01.295"},{"key":"Ref17","unstructured":"EUCAST (European Committee on Antimicrobial Susceptibility Testing) (2021) MIC distributions and epidemiological cut-off value (ECOFF) setting, EUCAST SOP 10.2. www.eucast.org."},{"key":"Ref18","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.846819"},{"key":"Ref19","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.1998.tb01128.x"},{"key":"Ref20","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.023051-0"},{"key":"Ref21","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02600"},{"key":"Ref22","doi-asserted-by":"publisher","DOI":"10.2166\/wst.1991.0096"},{"key":"Ref23","doi-asserted-by":"publisher","DOI":"10.1111\/raq.12460"},{"key":"Ref24","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.11.4145-4151.2000"},{"key":"Ref25","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.13612"},{"key":"Ref26","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0008607"},{"key":"Ref27","doi-asserted-by":"publisher","DOI":"10.20506\/rst.31.1.2097"},{"key":"Ref28","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2018.02.020"},{"key":"Ref29","doi-asserted-by":"publisher","DOI":"10.3354\/dao03745"},{"key":"Ref30","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1900141116"},{"key":"Ref31","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.15040"},{"key":"Ref32","unstructured":"WHO (2019) Critically important antimicrobials for human medicine, 6th revision. World Health Organisation, Geneva"}],"container-title":["Diseases of Aquatic Organisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.int-res.com\/articles\/dao_oa\/d156p115.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.int-res.com\/articles\/dao_oa\/d156p115.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T13:53:31Z","timestamp":1702562011000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.int-res.com\/abstracts\/dao\/v156\/p115-121\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,14]]},"references-count":32,"URL":"https:\/\/doi.org\/10.3354\/dao03766","relation":{},"ISSN":["0177-5103","1616-1580"],"issn-type":[{"value":"0177-5103","type":"print"},{"value":"1616-1580","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12,14]]},"assertion":[{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}