{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:32:43Z","timestamp":1790616763445,"version":"4.1.0"},"reference-count":72,"publisher":"Inter-Research Science Center","license":[{"start":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T00:00:00Z","timestamp":1722470400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["int-res.com"],"crossmark-restriction":false},"short-container-title":["Dis. Aquat. Org."],"abstract":"<jats:p>The chytrid <jats:italic>Batrachochytrium dendrobatidis<\/jats:italic> (<jats:italic>B<\/jats:italic><jats:italic>d<\/jats:italic>) is a widespread fungus causing amphibian declines across the globe. Although data on <jats:italic>Bd<\/jats:italic> occurrence in Eastern Europe are scarce, a recent species distribution model (SDM) for <jats:italic>Bd<\/jats:italic> reported that western and north-western parts of Ukraine are highly suitable to the pathogen. We verified the SDM-predicted range of <jats:italic>Bd<\/jats:italic> in Ukraine by sampling amphibians across the country and screening for <jats:italic>Bd<\/jats:italic> using qPCR. A total of 446 amphibian samples (tissue and skin swabs) from 11 species were collected from 36 localities. We obtained qPCR-positive results for 33 samples including waterfrogs (<jats:italic>Pelophylax esculentus<\/jats:italic> complex) and fire- and yellow-bellied toads (<jats:italic>Bombina<\/jats:italic> spp.) from 8 localities. We found that <jats:italic>Bd<\/jats:italic>-positive localities had significantly higher predicted <jats:italic>Bd<\/jats:italic> habitat suitability than sites that were pathogen-free. Amplification and sequencing of the internal transcribed spacer (ITS) region of samples with the highest <jats:italic>Bd<\/jats:italic> load revealed matches with ITS haplotypes of the globally distributed <jats:italic>Bd<\/jats:italic>GPL strain, and a single case of the <jats:italic>Bd<\/jats:italic>ASIA-2\/<jats:italic>Bd<\/jats:italic>BRAZIL haplotype. We found that <jats:italic>Bd<\/jats:italic> was non-randomly distributed across Ukraine, with infections present in the western and north-central forested peripheries of the country with a relatively cool, moist climate. On the other hand, our results suggest that <jats:italic>Bd<\/jats:italic> is absent or present in low abundance in the more continental central, southern and eastern regions of Ukraine, corroborating the model-predicted distribution of chytrid fungus. These areas could potentially serve as climatic refugia for <jats:italic>Bd<\/jats:italic>-susceptible amphibian hosts.<\/jats:p>","DOI":"10.3354\/dao03802","type":"journal-article","created":{"date-parts":[[2024,6,28]],"date-time":"2024-06-28T13:51:44Z","timestamp":1719582704000},"page":"15-27","update-policy":"https:\/\/doi.org\/10.3354\/policy","source":"Crossref","is-referenced-by-count":6,"title":["Countrywide screening supports model-based predictions of the distribution of Batrachochytrium dendrobatidis in Ukraine"],"prefix":"10.3354","volume":"159","author":[{"given":"J","family":"Jak\u00f3bik","sequence":"first","affiliation":[{"name":"Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University, 30-387 Krak\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Drohvalenko","sequence":"additional","affiliation":[{"name":"Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University, 30-387 Krak\u00f3w, Poland"},{"name":"Zoology and Animal Ecology Department, School of Biology, V. N. Karazin Kharkiv National University, 61022 Kharkiv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Fernandez Melendez","sequence":"additional","affiliation":[{"name":"Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University, 30-387 Krak\u00f3w, Poland"},{"name":"Department of Pathobiology, Pharmacology and Zoological Medicine, Faculty of Veterinary Medicine, 9820 Merelbeke, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"K\u0119pa","sequence":"additional","affiliation":[{"name":"Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University, 30-387 Krak\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O","family":"Klynova","sequence":"additional","affiliation":[{"name":"Department of Mycology and Plant Resistance, School of Biology, V. N. Karazin Kharkiv National University, 61022 Kharkiv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Fedorova","sequence":"additional","affiliation":[{"name":"Zoology and Animal Ecology Department, School of Biology, V. N. Karazin Kharkiv National University, 61022 Kharkiv, Ukraine"},{"name":"Laboratory of Fish Genetics, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, 277 21 Lib\u011bchov, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O","family":"Korshunov","sequence":"additional","affiliation":[{"name":"Zoology and Animal Ecology Department, School of Biology, V. N. Karazin Kharkiv National University, 61022 Kharkiv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O","family":"Marushchak","sequence":"additional","affiliation":[{"name":"I. I. Schmalhausen Institute of Zoology, National Academy of Sciences of Ukraine, 01054 Kyiv, Ukraine"},{"name":"Universit\u00e9 de Strasbourg, CNRS, IPHC UMR 7178, 67000 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O","family":"Nekrasova","sequence":"additional","affiliation":[{"name":"I. I. Schmalhausen Institute of Zoology, National Academy of Sciences of Ukraine, 01054 Kyiv, Ukraine"},{"name":"Universit\u00e9 de Strasbourg, CNRS, IPHC UMR 7178, 67000 Strasbourg, France"},{"name":"Department of Ecology, Institute of Life Sciences and Technologies, Daugavpils University, 5401 Daugavpils, Latvia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N","family":"Suriadna","sequence":"additional","affiliation":[{"name":"Melitopol Institute of Ecology and Social Technologies of the University Ukraine, 04071 Kyiv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N","family":"Smirnov","sequence":"additional","affiliation":[{"name":"Chernivtsi Regional Museum of Local Lore, 58002 Chernivtsi, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O","family":"Tkachenko","sequence":"additional","affiliation":[{"name":"T.H. Shevchenko National University Chernihiv Colehium, 14013 Chernihiv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Tupikov","sequence":"additional","affiliation":[{"name":"Society for Conservation GIS Ukraine, Svobody sq. 4, 61022 Kharkiv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Dufresnes","sequence":"additional","affiliation":[{"name":"Institut de Syst\u00e9matique, Evolution, Biodiversit\u00e9, Mus\u00e9um national d\u2019Histoire naturelle, CNRS, Sorbonne Universit\u00e9, EPHE-PSL, Universit\u00e9 des Antilles, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O","family":"Zinenko","sequence":"additional","affiliation":[{"name":"Department of Mycology and Plant Resistance, School of Biology, V. N. 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Springer, Cham, p 59-\u206070","DOI":"10.1007\/978-3-319-13755-1_3"},{"key":"Ref25","doi-asserted-by":"publisher","DOI":"10.3201\/eid0908.030145"},{"key":"Ref26","doi-asserted-by":"publisher","DOI":"10.1111\/csp2.12978"},{"key":"Ref27","doi-asserted-by":"publisher","DOI":"10.1163\/15685381-bja10031"},{"key":"Ref28","doi-asserted-by":"publisher","DOI":"10.3354\/dao03121"},{"key":"Ref29","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-4642.2007.00394.x"},{"key":"Ref30","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7998.2006.00220.x"},{"key":"Ref31","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2015.0465"},{"key":"Ref32","doi-asserted-by":"crossref","unstructured":"Liu X, Rohr JR, Li Y (2013) Climate, vegetation, introduced hosts and trade shape a global wildlife pandemic. 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Nature 516:158-161","DOI":"10.1038\/516158a"},{"key":"Ref39","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1739.2009.01211.x"},{"key":"Ref40","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-28304-1"},{"key":"Ref41","doi-asserted-by":"publisher","DOI":"10.1126\/science.aar1965"},{"key":"Ref42","doi-asserted-by":"publisher","DOI":"10.3354\/dao02662"},{"key":"Ref43","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0056802"},{"key":"Ref44","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1739.2005.00108.x"},{"key":"Ref45","doi-asserted-by":"publisher","DOI":"10.1111\/eva.12940"},{"key":"Ref46","doi-asserted-by":"publisher","DOI":"10.3354\/dao03631"},{"key":"Ref47","doi-asserted-by":"publisher","DOI":"10.1007\/s10531-022-02525-3"},{"key":"Ref48","doi-asserted-by":"publisher","DOI":"10.1080\/15572536.2005.11832990"},{"key":"Ref49","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-4642.2008.00548.x"},{"key":"Ref50","unstructured":"Pysanets Ye (2012). Amphibians of the [sic] Eastern Europe. Part I. Order Caudata. Zoological museum NNPM NAS Ukraine, Kyiv"},{"key":"Ref51","doi-asserted-by":"publisher","DOI":"10.3354\/dao075201"},{"key":"Ref52","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.3632"},{"key":"Ref53","doi-asserted-by":"publisher","DOI":"10.3390\/d1010052"},{"key":"Ref54","doi-asserted-by":"publisher","DOI":"10.1002\/eap.1607"},{"key":"Ref55","doi-asserted-by":"publisher","DOI":"10.1126\/science.aav0379"},{"key":"Ref56","unstructured":"Scherer R (2018) PropCIs: various confidence interval methods for proportions. R package version 0.3-0. https:\/\/cran.r-project.org\/package=PropCIs"},{"key":"Ref57","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294X.2012.05710.x"},{"key":"Ref58","doi-asserted-by":"publisher","DOI":"10.1111\/ecog.00733"},{"key":"Ref59","doi-asserted-by":"publisher","DOI":"10.3354\/dao01923"},{"key":"Ref60","doi-asserted-by":"publisher","DOI":"10.3354\/dao03230"},{"key":"Ref61","doi-asserted-by":"publisher","DOI":"10.1126\/science.1103538"},{"key":"Ref62","doi-asserted-by":"crossref","unstructured":"Suriadna N, Mykytynets H (2023) Amphibians of the genus Rana in the steppe zone of Ukraine: population status, distribution, and ecological features. GEO & BIO 25:187-\u2060201","DOI":"10.53452\/gb2515"},{"key":"Ref63","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msab120"},{"key":"Ref64","doi-asserted-by":"crossref","unstructured":"Thompson JD, Gibson TJ, Higgins DG (2003) Multiple sequence alignment using ClustalW and ClustalX. Curr Protoc Bioinf 1:2.3.1-\u20602.3.22","DOI":"10.1002\/0471250953.bi0203s00"},{"key":"Ref65","doi-asserted-by":"publisher","DOI":"10.3390\/jof9060607"},{"key":"Ref66","unstructured":"Vojar J, Havl\u00edkov\u00e1 B, Solsk\u00fd M, Jablonski D, Ikovi\u0107 V, Bal\u00e1\u017e V (2017) Distribution, prevalence and amphibian hosts of Batrachochytrium dendrobatidis in the Balkans. Salamandra 53:44-\u206049"},{"key":"Ref67","unstructured":"V\u00f6r\u00f6s J, Bosch J, D\u00e1n \u00c1, Hartel T (2013) First record of Batrachochytrium dendrobatidis on amphibians in Romania. North-West J Zool 9: 446-449"},{"key":"Ref68","unstructured":"V\u00f6r\u00f6s J, Herczeg D, F\u00fcl\u00f6p A, G\u00e1l TJ, D\u00e1n \u00c1, Harmos K, Bosch J (2018) Batrachochytrium dendrobatidis in Hungary: an overview of recent and historical occurrence. Acta Herpetol 13:125-\u2060140"},{"key":"Ref69","doi-asserted-by":"crossref","unstructured":"Wake DB, Vredenburg VT (2008) Are we in the midst of the sixth mass extinction? A view from the world of amphibians. 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