{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T08:03:02Z","timestamp":1790755382111,"version":"4.1.0"},"reference-count":36,"publisher":"Inter-Research Science Center","license":[{"start":{"date-parts":[[2024,8,15]],"date-time":"2024-08-15T00:00:00Z","timestamp":1723680000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.int-res.com\/journals\/terms-of-use\/"}],"content-domain":{"domain":["int-res.com"],"crossmark-restriction":false},"short-container-title":["Dis. Aquat. Org."],"abstract":"<jats:p>Piscine francisellosis is one of the most important bacterial diseases affecting various fish species worldwide. <jats:italic>Francisella orientalis, F. noatunensis<\/jats:italic>, and <jats:italic>F. salimarina<\/jats:italic> (<jats:italic>F. marina<\/jats:italic>) have been reported as etiological agents of disease in fish. A <jats:italic>Francisella<\/jats:italic> sp. was isolated from several diseased red drum <jats:italic>Sciaenops ocellatus<\/jats:italic> experiencing morbidity in Florida, USA, in 2008. In this study, molecular and phenotypic characterization of the recovered isolate was conducted. Phenotypically, the isolate showed a biochemical reaction profile distinct from that of <jats:italic>F. orientalis<\/jats:italic> and <jats:italic>F. salimarina<\/jats:italic>. Although the 16S rRNA sequence of this isolate shared 99.61% identity to the type strain of <jats:italic>F. philomiragia<\/jats:italic> O#319L<jats:sup>T<\/jats:sup>, whole genome analysis (average nucleotide identity &lt;95%; digital DNA-DNA hybridization &lt;70%) and a multilocus sequence analysis of 8 concatenated housekeeping genes in comparison with other <jats:italic>Francisella<\/jats:italic> spp. indicated that this isolate was a novel <jats:italic>Francisella<\/jats:italic> species, more closely related to <jats:italic>F. orientalis<\/jats:italic>. Immersion, intracoelomic injection, and co-habitation challenges using a Nile tilapia <jats:italic>Oreochromis niloticus<\/jats:italic> fingerling model of infection were done to investigate virulence in a piscine model. Variably pigmented granulomas and pigmented macrophage aggregates were observed in the kidneys and spleens of the challenged fish, but no mortality was recorded during the 15 d challenge period, suggesting that this novel <jats:italic>Francisella<\/jats:italic> sp. might be an opportunistic pathogen of fish. Based on the phenotypic and genotypic differences from other <jats:italic>Francisella<\/jats:italic> spp. observed in this study, we propose the name <jats:italic>Francisella sciaenopsi<\/jats:italic> sp. nov. for this novel isolate.<\/jats:p>","DOI":"10.3354\/dao03803","type":"journal-article","created":{"date-parts":[[2024,6,28]],"date-time":"2024-06-28T13:51:44Z","timestamp":1719582704000},"page":"79-89","update-policy":"https:\/\/doi.org\/10.3354\/policy","source":"Crossref","is-referenced-by-count":2,"title":["Francisella sciaenopsi sp. nov. isolated from diseased red drum Sciaenops ocellatus in Florida, USA"],"prefix":"10.3354","volume":"159","author":[{"given":"M","family":"Kawahara","sequence":"first","affiliation":[{"name":"Department of Medicine & Epidemiology, School of Veterinary Medicine, University of California-Davis, One Shields Avenue, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"TT","family":"Cody","sequence":"additional","affiliation":[{"name":"Fish and Wildlife Health, Fish and Wildlife Research Institute, Florida Fish and Wildlife Conservation Commission, St. Petersburg, Florida 33701, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"RPE","family":"Yanong","sequence":"additional","affiliation":[{"name":"Tropical Aquaculture Laboratory, Fisheries and Aquatic Sciences Program, School of Forest, Fisheries, and Geomatics Sciences, Institute of Food and Agricultural Sciences, University of Florida, Ruskin, Florida 33570, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Henderson","sequence":"additional","affiliation":[{"name":"California Animal Health and Food Safety Lab, School of Veterinary Medicine, University of California-Davis, San Bernardino, CA 92408, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z","family":"Yazdi","sequence":"additional","affiliation":[{"name":"Department of Medicine & Epidemiology, School of Veterinary Medicine, University of California-Davis, One Shields Avenue, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Soto","sequence":"additional","affiliation":[{"name":"Department of Medicine & Epidemiology, School of Veterinary Medicine, University of California-Davis, One Shields Avenue, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1913","published-online":{"date-parts":[[2024,8,15]]},"reference":[{"key":"Ref1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0110726"},{"key":"Ref2","doi-asserted-by":"publisher","DOI":"10.1038\/srep23080"},{"key":"Ref3","unstructured":"Atlantic States Marine Fisheries Commission (2024) Red drum. https:\/\/www.asmfc.org\/species\/red-drum (accessed 26 January 2024)"},{"key":"Ref4","unstructured":"Bert TM, McMichael RH Jr, Cody RP, Forstchen AB and others (2003) Evaluating stock enhancement strategies: a multi-disciplinary approach. 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Haworth Press (Taylor & Francis Group), Binghamton, NY"}],"container-title":["Diseases of Aquatic Organisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.int-res.com\/articles\/dao2024\/159\/d159p079.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.int-res.com\/articles\/dao2024\/159\/d159p079.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,15]],"date-time":"2024-08-15T10:21:20Z","timestamp":1723717280000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.int-res.com\/abstracts\/dao\/v159\/p79-89\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,15]]},"references-count":36,"URL":"https:\/\/doi.org\/10.3354\/dao03803","relation":{},"ISSN":["0177-5103","1616-1580"],"issn-type":[{"value":"0177-5103","type":"print"},{"value":"1616-1580","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,15]]}}}