{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T17:22:37Z","timestamp":1787764957358,"version":"build-2784847793"},"reference-count":22,"publisher":"Inter-Research Science Center","license":[{"start":{"date-parts":[[2024,10,10]],"date-time":"2024-10-10T00:00:00Z","timestamp":1728518400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["int-res.com"],"crossmark-restriction":false},"short-container-title":["Dis. Aquat. Org."],"abstract":"<jats:p>Acute hepatopancreatic necrosis disease (AHPND) is a devastating shrimp disease caused by a binary toxin, PirAB, produced by <jats:italic>Vibrio parahaemolyticus<\/jats:italic> and other closely related bacteria. To address AHPND, over 300 unique single-domain antibodies (also known as nanobodies) derived from the V<jats:sub>H<\/jats:sub>H domains of <jats:italic>Lama glama<\/jats:italic> heavy-chain-only antibodies were raised against either PirA or PirB and characterized. Nanobodies were shortlisted based on their affinities for either PirA or PirB, their relative stability in intestinal fluids, and their ability to reduce PirAB-induced death in brine shrimp <jats:italic>Artemia salina<\/jats:italic>. From these data, a subset of nanobodies was tested for their ability to reduce AHPND in whiteleg shrimp <jats:italic>Penaeus vannamei<\/jats:italic>, and nanobodies targeting either PirA or PirB provided significant disease protection to whiteleg shrimp. These results show that nanobodies can be a new option for shrimp farmers to reduce or eliminate the impact of AHPND on their operations.<\/jats:p>","DOI":"10.3354\/dao03817","type":"journal-article","created":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T08:49:50Z","timestamp":1724316590000},"page":"7-12","update-policy":"https:\/\/doi.org\/10.3354\/policy","source":"Crossref","is-referenced-by-count":2,"title":["PirA- or PirB-binding nanobodies can protect whiteleg shrimp from the acute hepatopancreatic necrosis disease toxin"],"prefix":"10.3354","volume":"160","author":[{"given":"S","family":"Cheung","sequence":"first","affiliation":[{"name":"NovoBind Livestock Therapeutics, Vancouver, BC V6E 0C3, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SA","family":"Loutet","sequence":"additional","affiliation":[{"name":"NovoBind Livestock Therapeutics, Vancouver, BC V6E 0C3, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Zaytsoff","sequence":"additional","affiliation":[{"name":"NovoBind Livestock Therapeutics, Vancouver, BC V6E 0C3, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F","family":"Van Petegem","sequence":"additional","affiliation":[{"name":"University of British Columbia, Department of Biochemistry and Molecular Biology, Vancouver, BC V6T 1Z3, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"LH","family":"Tran","sequence":"additional","affiliation":[{"name":"ShrimpVet Laboratory, Nong Lam University, Ho Chi Minh City 720371, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Abnousi","sequence":"additional","affiliation":[{"name":"NovoBind Livestock Therapeutics, Vancouver, BC V6E 0C3, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1913","published-online":{"date-parts":[[2024,10,10]]},"reference":[{"key":"Ref1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00870-15"},{"key":"Ref2","doi-asserted-by":"publisher","DOI":"10.1002\/0471142735.im0217s103"},{"key":"Ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2022.06.042"},{"key":"Ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2022.104003"},{"key":"Ref5","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.1270"},{"key":"Ref6","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17050723"},{"key":"Ref7","doi-asserted-by":"publisher","DOI":"10.3354\/dao02830"},{"key":"Ref8","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0208978"},{"key":"Ref9","doi-asserted-by":"publisher","DOI":"10.3390\/toxins13080524"},{"key":"Ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2017.12.062"},{"key":"Ref11","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1503129112"},{"key":"Ref12","doi-asserted-by":"publisher","DOI":"10.1038\/s43016-020-0029-6"},{"key":"Ref13","doi-asserted-by":"publisher","DOI":"10.3390\/md15120373"},{"key":"Ref14","doi-asserted-by":"publisher","DOI":"10.3390\/toxins11040233"},{"key":"Ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.psj.2024.103578"},{"key":"Ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2008.08.010"},{"key":"Ref17","doi-asserted-by":"publisher","DOI":"10.1007\/s12010-009-8555-6"},{"key":"Ref18","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-063011-092449"},{"key":"Ref19","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13024"},{"key":"Ref20","doi-asserted-by":"publisher","DOI":"10.3354\/dao02621"},{"key":"Ref21","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0070-x"},{"key":"Ref22","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiu196"}],"container-title":["Diseases of Aquatic Organisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.int-res.com\/articles\/dao_oa\/d160p007.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.int-res.com\/articles\/dao_oa\/d160p007.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,10]],"date-time":"2024-10-10T12:10:45Z","timestamp":1728562245000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.int-res.com\/abstracts\/dao\/v160\/p7-12\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,10]]},"references-count":22,"URL":"https:\/\/doi.org\/10.3354\/dao03817","relation":{},"ISSN":["0177-5103","1616-1580"],"issn-type":[{"value":"0177-5103","type":"print"},{"value":"1616-1580","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,10]]},"assertion":[{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}