{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T09:18:38Z","timestamp":1759742318851,"version":"3.40.5"},"reference-count":43,"publisher":"Inter-Research Science Center","license":[{"start":{"date-parts":[[2025,1,9]],"date-time":"2025-01-09T00:00:00Z","timestamp":1736380800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.int-res.com\/journals\/terms-of-use\/"}],"content-domain":{"domain":["int-res.com"],"crossmark-restriction":false},"short-container-title":["Dis. Aquat. Org."],"abstract":"<jats:p>Cyvirus cyprinidallo 2 (CyHV-2) is the pathogen of herpesviral hematopoietic necrosis (HVHN) that mainly infects goldfish <jats:italic>Carassius auratus<\/jats:italic> and crucian carp <jats:italic>C. carassius<\/jats:italic> and is characterized by high infectivity and pathogenicity. The availability of rapid and convenient detection methods is essential for early detection of CyHV-2. A colloidal gold immunochromatographic strip using 2 specific anti-CyHV-2 monoclonal antibodies (MAbs) has been developed and validated for rapid detection of CyHV-2. The test results can be viewed within 10min. The detection limit of test strip was 2.08 \u00d7 10<jats:sup>2<\/jats:sup>TCID<jats:sub>50<\/jats:sub> ml<jats:sup>-1<\/jats:sup>, and it showed no cross-reactivity with other freshwater fish viral pathogen except KHV (koi herpesvirus). The specificity of the strip was 100% when spleen and kidney tissues of CyHV-2 infected and healthy crucian carp were assayed following an experimental challenge. The colloidal gold immunochromatographic strip will be an effective device for the rapid detection of CyHV-2 in the future.<\/jats:p>","DOI":"10.3354\/dao03833","type":"journal-article","created":{"date-parts":[[2024,11,18]],"date-time":"2024-11-18T14:28:45Z","timestamp":1731940125000},"page":"1-9","update-policy":"https:\/\/doi.org\/10.3354\/policy","source":"Crossref","is-referenced-by-count":1,"title":["Development of colloidal gold immunochromatographic strip for rapid detection of cyvirus cyprinidallo 2"],"prefix":"10.3354","volume":"161","author":[{"given":"L","family":"Zhao","sequence":"first","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, 201306 Shanghai, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Wang","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, 201306 Shanghai, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X","family":"Liu","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, 201306 Shanghai, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y","family":"Zhou","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, 201306 Shanghai, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Li","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, 201306 Shanghai, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Lu","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, 201306 Shanghai, PR China"},{"name":"Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, 201306 Shanghai, PR China"},{"name":"National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, 201306 Shanghai, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y","family":"Jiang","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, 201306 Shanghai, PR China"},{"name":"Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, 201306 Shanghai, PR China"},{"name":"National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, 201306 Shanghai, PR China"},{"name":"College of Fisheries and Life Science, Shanghai Ocean University, 201306 Shanghai, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1913","published-online":{"date-parts":[[2025,1,9]]},"reference":[{"key":"Ref1","doi-asserted-by":"publisher","DOI":"10.3147\/jsfp.34.209"},{"key":"Ref2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.03206-12"},{"key":"Ref3","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12048"},{"key":"Ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2020.735184"},{"key":"Ref5","doi-asserted-by":"publisher","DOI":"10.3354\/dao069137"},{"key":"Ref6","doi-asserted-by":"publisher","DOI":"10.1577\/H05-007.1"},{"key":"Ref7","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2008.00988.x"},{"key":"Ref8","doi-asserted-by":"publisher","DOI":"10.1177\/104063879801000415"},{"key":"Ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2021.114342"},{"key":"Ref10","doi-asserted-by":"publisher","DOI":"10.3354\/dao03561"},{"key":"Ref11","doi-asserted-by":"publisher","DOI":"10.12717\/DR.2020.24.4.277"},{"key":"Ref12","doi-asserted-by":"publisher","DOI":"10.3354\/dao02627"},{"key":"Ref13","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2007.00847.x"},{"key":"Ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2020.735156"},{"key":"Ref15","doi-asserted-by":"publisher","DOI":"10.1093\/jaoacint\/qsaa152"},{"key":"Ref16","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12648"},{"key":"Ref17","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13868"},{"key":"Ref18","doi-asserted-by":"crossref","unstructured":"Li G, Wang X, Li Q, Yang J and others (2021) Development of an immunochromatographic strip for rapid detection of H7 subtype avian influenza viruses. 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