{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T21:40:40Z","timestamp":1790199640258,"version":"4.1.0"},"reference-count":13,"publisher":"Inter-Research Science Center","license":[{"start":{"date-parts":[[2025,2,20]],"date-time":"2025-02-20T00:00:00Z","timestamp":1740009600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.int-res.com\/journals\/terms-of-use\/"}],"content-domain":{"domain":["int-res.com"],"crossmark-restriction":false},"short-container-title":["Dis. Aquat. Org."],"abstract":"<jats:p>Viral hemorrhagic septicemia virus (VHSV) was detected and identified in healthy bastard halibut <jats:italic>Paralichthys olivaceus<\/jats:italic> on the east coast of China through a combination of virus isolation, real-time reverse transcription polymerase chain reaction (RT-PCR) and conventional RT-PCR followed by sequencing. Analysis using MEGA11 software revealed that the isolated VHSV belongs to genotype IVa. Bastard halibut fry were experimentally infected by immersion and gill inoculation with infected cell culture medium and exhibited clinical signs. Cytopathic effects (CPE) were observed again when the epithelioma papulosum cyprini (EPC) cells were inoculated with homogenized tissue samples from the diseased fish. Real-time RT-PCR confirmed the existence of the virus in the infected fish tissues. Transmission electron microscopy (TEM) revealed the presence of numerous virus particles in the infected EPC cells, both extracellularly and intracellularly. The combination of clinical signs, typical CPE, and genetic analysis identified the isolated virus as VHSV.<\/jats:p>","DOI":"10.3354\/dao03840","type":"journal-article","created":{"date-parts":[[2025,1,15]],"date-time":"2025-01-15T10:52:38Z","timestamp":1736938358000},"page":"69-74","update-policy":"https:\/\/doi.org\/10.3354\/policy","source":"Crossref","is-referenced-by-count":3,"title":["Isolation and characterization of viral hemorrhagic septicemia virus from farmed bastard halibut"],"prefix":"10.3354","volume":"161","author":[{"given":"Y","family":"Xu","sequence":"first","affiliation":[{"name":"Comprehensive Technology Center, Lianyungang Customs, Lianyungang, Jiangsu, 222000, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Tang","sequence":"additional","affiliation":[{"name":"Animal, Plant and Food Inspection Center, Nanjing Customs District, Nanjing, Jiangsu, 210019, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Wang","sequence":"additional","affiliation":[{"name":"Comprehensive Technology Center, Lianyungang Customs, Lianyungang, Jiangsu, 222000, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W","family":"Dou","sequence":"additional","affiliation":[{"name":"Comprehensive Technology Center, Lianyungang Customs, Lianyungang, Jiangsu, 222000, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N","family":"Wang","sequence":"additional","affiliation":[{"name":"Chinese Academy of Inspection and Quarantine, Beijing, 100176, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Zhou","sequence":"additional","affiliation":[{"name":"Comprehensive Technology Center, Lianyungang Customs, Lianyungang, Jiangsu, 222000, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Duan","sequence":"additional","affiliation":[{"name":"Comprehensive Technology Center, Lianyungang Customs, Lianyungang, Jiangsu, 222000, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1913","published-online":{"date-parts":[[2025,2,20]]},"reference":[{"key":"Ref1","doi-asserted-by":"publisher","DOI":"10.3354\/dao03618"},{"key":"Ref2","doi-asserted-by":"publisher","DOI":"10.3354\/dao02344"},{"key":"Ref3","doi-asserted-by":"publisher","DOI":"10.3354\/dao02588"},{"key":"Ref4","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12910"},{"key":"Ref5","doi-asserted-by":"publisher","DOI":"10.3724\/issn1000-3207-2007-3-345-t"},{"key":"Ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2018.03.047"},{"key":"Ref7","doi-asserted-by":"publisher","DOI":"10.1016\/0959-8030(95)00002-X"},{"key":"Ref8","doi-asserted-by":"publisher","DOI":"10.3354\/dao02728"},{"key":"Ref9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0135146"},{"key":"Ref10","unstructured":"Sun YJ, Jiang YL, Liu H, Gao LY, Shi XJ, He JQ, Wang Z (2009) The isolation and characterization of a rhabdovirus from stone flounder, Kareius bicoloratus. Chin J Vet Sci 29:277-282 (in Chinese with English abstract)"},{"key":"Ref11","unstructured":"Walker PJ, Benmansour A, Dietzgen R (2000) Family Rhabdoviridae. In: Van Regenmortel MHV, Fauquet CM, Bishop DHL and others (eds) Virus taxonomy. Classification and nomenclature of viruses. Seventh Report of the International Committee on Taxonomy of Viruses. Academic Press, New York, NY, p 563-583"},{"key":"Ref12","unstructured":"WOAH (World Organization for Animal Health) (2023) Infection with viral haemorrhagic septicaemia virus. In: Olesen NJ, Garver K, Kim HJ (eds) Manual of diagnostic tests for aquatic animals, Chap 2.3.10. World Organization for Animal Health, Paris, p 377-401"},{"key":"Ref13","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-013-1716-5"}],"container-title":["Diseases of Aquatic Organisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.int-res.com\/articles\/dao2025\/161\/d161p069.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.int-res.com\/articles\/dao2025\/161\/d161p069.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T10:52:38Z","timestamp":1740135158000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.int-res.com\/abstracts\/dao\/v161\/p69-74\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,20]]},"references-count":13,"URL":"https:\/\/doi.org\/10.3354\/dao03840","relation":{},"ISSN":["0177-5103","1616-1580"],"issn-type":[{"value":"0177-5103","type":"print"},{"value":"1616-1580","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,20]]}}}