{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T10:30:58Z","timestamp":1764844258947,"version":"3.46.0"},"reference-count":20,"publisher":"Inter-Research Science Center","license":[{"start":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T00:00:00Z","timestamp":1764806400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.int-res.com\/for-librarians\/reader-subscriber-license"}],"content-domain":{"domain":["int-res.com"],"crossmark-restriction":false},"short-container-title":["Dis. Aquat. Org."],"accepted":{"date-parts":[[2025,8,19]]},"abstract":"<jats:p>\n                    This study analysed published data on the distributions of minimum inhibitory concentrations of a group of freshwater isolates classified as\n                    <jats:italic>Aeromonas<\/jats:italic>\n                    spp. with the aim of establishing whether they provided any evidence that epidemiological cut-off values set from these data would be unreliable. This group contained 233 isolates and included members of at least 11 species. The standard deviations (SDs) of the wild-type distributions for 10 antimicrobial agents were calculated for this multi-species group using the ECOFFinder and normalised resistance interpretation (NRI) algorithms. These were compared to the SDs of 110 distributions established for individual species published by the European Committee on Antimicrobial Susceptibility Testing. Fifty-one of these distributions had been generated by multiple laboratories and 59 by single laboratories. When the ECOFFinder algorithm was used to calculate the SDs, the mean for the multi-species group was 0.63 log\n                    <jats:sub>2<\/jats:sub>\n                    \u00b5g ml\n                    <jats:sup>-1<\/jats:sup>\n                    , and the 51 individual species and multiple-laboratory groups were 0.68 and 0.65 log\n                    <jats:sub>2<\/jats:sub>\n                    \u00b5g ml\n                    <jats:sup>-1<\/jats:sup>\n                    , respectively. When the NRI algorithm was used, the mean for the multi-species group was 0.79 log\n                    <jats:sub>2<\/jats:sub>\n                    \u00b5g ml\n                    <jats:sup>-1<\/jats:sup>\n                    , and the 51 individual species and multiple-laboratory groups were 0.79 and 0.76 log\n                    <jats:sub>2<\/jats:sub>\n                    \u00b5g ml\n                    <jats:sup>-1<\/jats:sup>\n                    , respectively. These comparisons indicate that the heterogeneity in the susceptibility to antimicrobial agents within the multi-species group of\n                    <jats:italic>Aeromonas<\/jats:italic>\n                    is not significantly different from that recorded for individual species. This analysis, therefore, suggests that epidemiological cut-off values designed to be applied to all members of the genus\n                    <jats:italic>Aeromonas<\/jats:italic>\n                    would not be inherently unreliable.\n                  <\/jats:p>","DOI":"10.3354\/dao03878","type":"journal-article","created":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T08:14:33Z","timestamp":1757405673000},"page":"143-148","update-policy":"https:\/\/doi.org\/10.3354\/policy","source":"Crossref","is-referenced-by-count":0,"title":["Feasibility of setting epidemiological cut-off values for Aeromonas spp."],"prefix":"10.3354","volume":"164","author":[{"given":"Peter","family":"Smith","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandrine","family":"Baron","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Goran","family":"Kronvall","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1913","published-online":{"date-parts":[[2025,12,4]]},"reference":[{"key":"Ref1","doi-asserted-by":"publisher","DOI":"10.3109\/inf.1981.13.issue-2.13"},{"key":"Ref2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.00503"},{"key":"Ref3","unstructured":"CLSI (2006) Methods for antimicrobial disk susceptibility testing of bacteria isolated from aquatic animals. Approved guideline M42-A. Clinical and Laboratory Standards Institute, Wayne, PA"},{"key":"Ref4","unstructured":"CLSI (2006) Methods for broth dilution susceptibility testing of bacteria isolated from aquatic animals. Approved guideline M49-A. Clinical and Laboratory Standards Institute, Wayne, PA"},{"key":"Ref5","unstructured":"CLSI (2020) Methods for antimicrobial broth dilution and disk diffusion susceptibility testing of bacteria isolated from aquatic animals. CLSI guideline VET03, 2nd edn. Clinical Laboratory Standards Institute, Wayne, PA"},{"key":"Ref6","unstructured":"CLSI (2020) Performance standards for antimicrobial susceptibility testing of bacteria isolated from aquatic animals. CLSI guideline VET04, 3rd edn. Clinical Laboratory Standards Institute, Wayne, PA"},{"key":"Ref7","unstructured":"EUCAST (2021) SOP 10.2 MIC distributions and the setting of epidemiological cut-off (ECOFF) values. www.eucast.org\/eucastsops\/ (accessed 20 April 2024)"},{"key":"Ref8","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8010129"},{"key":"Ref9","unstructured":"Figueras MJ, Fern\u00e1ndez-Bravo A (2025) Introductory chapter: Aeromonas, the aquatic multipotential bacteria that may affect humans and animals - an overview of the past to face the future. In: Figueras MJ, Fern\u00e1ndez-Bravo A (eds) Aeromonas - a ubiquitous bacterium that infects humans and animals. IntechOpen, London. www.intechopen.com\/books\/1003505"},{"key":"Ref10","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00039-09"},{"key":"Ref11","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg312"},{"key":"Ref12","doi-asserted-by":"publisher","DOI":"10.2166\/wh.2009.041"},{"key":"Ref13","unstructured":"Kronvall G, Runehagen A (1981) Improved bacterial resistance testing by a standardized SIR-system. Lakartidningen 78:3483-3485"},{"key":"Ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2011.04.006"},{"key":"Ref15","unstructured":"Myhre E, Kronvall G (2000) High precision of disk tests reveals microheterogeneity of antibiotic susceptibility. Clin Microbiol Infect 6:109-110"},{"key":"Ref16","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12837"},{"key":"Ref17","unstructured":"Smith P (2019) The performance of antimicrobial susceptibility testing programmes relevant to aquaculture and aquaculture products. FAO Fisheries and Aquaculture Circular No. 1191. FAO, Rome"},{"key":"Ref18","doi-asserted-by":"publisher","DOI":"10.48045\/001c.38713"},{"key":"Ref19","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2018.02.020"},{"key":"Ref20","doi-asserted-by":"publisher","DOI":"10.3354\/dao03745"}],"container-title":["Diseases of Aquatic Organisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.int-res.com\/articles\/dao2025\/164\/d164p143.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.int-res.com\/articles\/dao2025\/164\/d164p143.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T10:29:39Z","timestamp":1764844179000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.int-res.com\/abstracts\/dao\/v164\/dao03878"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,4]]},"references-count":20,"URL":"https:\/\/doi.org\/10.3354\/dao03878","archive":["CLOCKSS"],"relation":{},"ISSN":["0177-5103","1616-1580"],"issn-type":[{"type":"print","value":"0177-5103"},{"type":"electronic","value":"1616-1580"}],"subject":[],"published":{"date-parts":[[2025,12,4]]}}}