{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T13:00:23Z","timestamp":1780578023373,"version":"3.54.1"},"reference-count":63,"publisher":"Inter-Research Science Center","license":[{"start":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T00:00:00Z","timestamp":1780531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.int-res.com\/for-librarians\/reader-subscriber-license"}],"content-domain":{"domain":["int-res.com"],"crossmark-restriction":false},"short-container-title":["Dis. Aquat. Org."],"accepted":{"date-parts":[[2026,3,6]]},"abstract":"<jats:p>\n                    Saprolegniosis is a common infectious disease caused by members of the genus\n                    <jats:italic>Saprolegnia<\/jats:italic>\n                    . This genus contains several species, some of which can cause severe infection in vulnerable fish such as rainbow trout\n                    <jats:italic>Oncorhynchus mykiss<\/jats:italic>\n                    . This study reports the isolation and identification of 4\n                    <jats:italic>Saprolegnia<\/jats:italic>\n                    species, namely\n                    <jats:italic>S. parasitica<\/jats:italic>\n                    ,\n                    <jats:italic>S. diclina<\/jats:italic>\n                    ,\n                    <jats:italic>S<\/jats:italic>\n                    .\n                    <jats:italic>aenigmatica<\/jats:italic>\n                    and\n                    <jats:italic>S. asterophora<\/jats:italic>\n                    , from a rainbow trout farm. The isolates were identified by amplification and sequencing of the internal transcribed spacer region. Among the isolates,\n                    <jats:italic>S. parasitica<\/jats:italic>\n                    and\n                    <jats:italic>S. diclina<\/jats:italic>\n                    were more common.\n                    <jats:italic>S. diclina<\/jats:italic>\n                    was found only in eggs and alevins, whereas\n                    <jats:italic>S. parasitica<\/jats:italic>\n                    was observed in different life stages of rainbow trout from fry to adult. Using the isolates, a species-specific PCR for the molecular identification of\n                    <jats:italic>S. parasitica<\/jats:italic>\n                    was optimised; the PCR protocol had a limit of detection of 0.025 ng template in a 20 \u00b5l reaction. Sensitivity of\n                    <jats:italic>S. parasitica<\/jats:italic>\n                    and\n                    <jats:italic>S. diclina<\/jats:italic>\n                    to boric acid and hydrogen peroxide was also determined; this assay also included another important\n                    <jats:italic>Saprolegnia<\/jats:italic>\n                    species (\n                    <jats:italic>S. australis<\/jats:italic>\n                    isolated from rainbow trout from a different rearing facility) to evaluate the comparative response among different species.\n                    <jats:italic>S. parasitica<\/jats:italic>\n                    and\n                    <jats:italic>S. diclina<\/jats:italic>\n                    showed similar sensitivity to boric acid, whereas\n                    <jats:italic>S. diclina<\/jats:italic>\n                    showed the highest sensitivity to hydrogen peroxide followed by\n                    <jats:italic>S. parasitica<\/jats:italic>\n                    and\n                    <jats:italic>S. australis.<\/jats:italic>\n                    This highlights the importance of accurate species identification for choosing suitable control measures and the judicious use of anti-oomycete drugs.\n                  <\/jats:p>","DOI":"10.3354\/dao03917","type":"journal-article","created":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T16:38:40Z","timestamp":1773765520000},"page":"1-11","update-policy":"https:\/\/doi.org\/10.3354\/policy","source":"Crossref","is-referenced-by-count":0,"title":["Saprolegnia species isolated from rainbow trout: molecular identification, specific PCR and drug sensitivity"],"prefix":"10.3354","volume":"166","author":[{"given":"Victoria Chanu","family":"Khangembam","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimpal","family":"Thakuria","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Garima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0545-6608","authenticated-orcid":true,"given":"Vinita","family":"Pant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5045-253X","authenticated-orcid":true,"given":"Kishor","family":"Kunal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1913","published-online":{"date-parts":[[2026,6,4]]},"reference":[{"key":"Ref1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0091878"},{"key":"Ref2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-54534-y"},{"key":"Ref3","doi-asserted-by":"publisher","DOI":"10.1126\/science.290.5493.972"},{"key":"Ref4","doi-asserted-by":"publisher","DOI":"10.2307\/2399880"},{"key":"Ref5","unstructured":"Beakes GW, Wood SE, Burr AW (1994) Features which characterize Saprolegnia isolates from salmonid fish lesions\u2014a review. 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