{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T11:52:48Z","timestamp":1781783568531,"version":"3.54.5"},"reference-count":90,"publisher":"Inter-Research Science Center","license":[{"start":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T00:00:00Z","timestamp":1781740800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.int-res.com\/for-librarians\/reader-subscriber-license"}],"content-domain":{"domain":["int-res.com"],"crossmark-restriction":false},"short-container-title":["Dis. Aquat. Org."],"accepted":{"date-parts":[[2026,4,7]]},"abstract":"<jats:p>\n                    Infectious diseases, particularly those caused by members of the genus\n                    <jats:italic>Ranavirus<\/jats:italic>\n                    (\n                    <jats:italic>Iridoviridae<\/jats:italic>\n                    ), have caused mass mortality events in amphibian populations worldwide. Frog virus 3 (FV3) infects a wide range of amphibian hosts and results in high mortality. Despite the global prevalence of FV3, its origin and routes of spread remain unclear, largely because of limited genetic data from limited geographic regions. This study aimed to classify the ranaviruses isolated in the Republic of Korea through whole-genome sequencing. We collected amphibian carcasses and both symptomatic and asymptomatic live amphibians. The asymptomatic amphibians were collected from locations with previous infections. Among the 278 amphibian samples collected from 24 locations, 44 were positive for ranavirus according to quantitative PCR (qPCR) analysis. We obtained whole-genome sequences from 5 isolates, all of which were identified as FV3-like ranaviruses and belonged to the Asian FV3 clade in the maximum likelihood phylogenetic tree. By comparative analysis of open reading frames (ORFs) and phylogenomic trees, the Korean isolates were further divided into 2 strains: FV3-Kor_MT (mountain lineage) and FV3-Kor_LL (lowland lineage). Our findings highlight the importance of surveillance of a wide range of amphibian species and facilitate phylogenetic analyses of global FV3-like strains, including newly verified Korean strains.\n                  <\/jats:p>","DOI":"10.3354\/dao03922","type":"journal-article","created":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T06:24:28Z","timestamp":1776147868000},"page":"1-15","update-policy":"https:\/\/doi.org\/10.3354\/policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification and phylogenetic analysis of Korean frog virus 3 (FV3)-like ranaviruses through whole-genome sequencing"],"prefix":"10.3354","volume":"166","author":[{"given":"Jongsun","family":"Kim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haan Woo","family":"Sung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tae Sung","family":"Jung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun Jin","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9209-0493","authenticated-orcid":true,"given":"Daesik","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1913","published-online":{"date-parts":[[2026,6,18]]},"reference":[{"key":"Ref1","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"Ref2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.12.2607"},{"key":"Ref3","doi-asserted-by":"publisher","DOI":"10.1080\/19768354.2015.1028442"},{"key":"Ref4","doi-asserted-by":"publisher","DOI":"10.1016\/bs.aivir.2018.02.005"},{"key":"Ref5","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-13755-1_4"},{"key":"Ref6","doi-asserted-by":"publisher","DOI":"10.3354\/dao03387"},{"key":"Ref7","doi-asserted-by":"publisher","DOI":"10.1139\/facets-2020-0013"},{"key":"Ref8","doi-asserted-by":"crossref","unstructured":"Brunner JL, Storfer A, Le Sage EH, Garner TW, Gray MJ, Hoverman JT (2024) Ranavirus ecology: from individual infections to population epidemiology to community impacts. In: Gray MJ, Chinchar VG (eds) Ranaviruses: emerging pathogens of ectothermic vertebrates. Springer, Cham, p 231-270","DOI":"10.1007\/978-3-031-64973-8_7"},{"key":"Ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2021.104200"},{"key":"Ref10","doi-asserted-by":"publisher","DOI":"10.1139\/facets-2020-0007"},{"key":"Ref11","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.05589-11"},{"key":"Ref12","doi-asserted-by":"publisher","DOI":"10.1186\/1297-9716-44-101"},{"key":"Ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2017.07.028"},{"key":"Ref14","unstructured":"Davis DR, Farkas JK, Kruisselbrink TR, Watters JL, Ellsworth ED, Kerby JL, Siler CD (2019) Prevalence and distribution of ranavirus in amphibians from southeastern Oklahoma, USA. Herpetol Conserv Biol 14:360-369"},{"key":"Ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2012.07.001"},{"key":"Ref16","doi-asserted-by":"publisher","DOI":"10.1007\/s10393-014-0950-y"},{"key":"Ref17","doi-asserted-by":"publisher","DOI":"10.1007\/s00442-016-3682-6"},{"key":"Ref18","doi-asserted-by":"publisher","DOI":"10.1002\/zoo.21180"},{"key":"Ref19","doi-asserted-by":"publisher","DOI":"10.3354\/dao072087"},{"key":"Ref20","doi-asserted-by":"publisher","DOI":"10.1111\/cobi.13938"},{"key":"Ref21","doi-asserted-by":"publisher","DOI":"10.30802\/AALAS-JAALAS-19-000141"},{"key":"Ref22","doi-asserted-by":"publisher","DOI":"10.32800\/abc.2009.32.0123"},{"key":"Ref23","doi-asserted-by":"publisher","DOI":"10.1007\/s10393-013-0856-0"},{"key":"Ref24","doi-asserted-by":"publisher","DOI":"10.3354\/dao03354"},{"key":"Ref25","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-13755-1_8"},{"key":"Ref26","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2002.tb04400.x"},{"key":"Ref27","doi-asserted-by":"publisher","DOI":"10.3354\/dao067009"},{"key":"Ref28","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2001.1245"},{"key":"Ref29","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2023.1291872"},{"key":"Ref30","doi-asserted-by":"publisher","DOI":"10.3354\/dao02200"},{"key":"Ref31","doi-asserted-by":"publisher","DOI":"10.1007\/s10393-011-0731-9"},{"key":"Ref32","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2021.736955"},{"key":"Ref33","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-10-224"},{"key":"Ref34","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-09575-2"},{"key":"Ref35","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4285"},{"key":"Ref36","doi-asserted-by":"publisher","DOI":"10.3391\/bir.2019.8.4.23"},{"key":"Ref37","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts199"},{"key":"Ref38","doi-asserted-by":"publisher","DOI":"10.5141\/jee.22.051"},{"key":"Ref39","doi-asserted-by":"publisher","DOI":"10.3390\/v16050675"},{"key":"Ref40","unstructured":"Kim S, Sim MY, Eom AH, Park DS, Ra NY (2009) PCR detection of ranavirus in gold-spotted pond frogs (Rana plancyi chosenica) from Korea. Korean J Environ Biol 27:110-113"},{"key":"Ref41","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2010.11.007"},{"key":"Ref42","doi-asserted-by":"publisher","DOI":"10.1007\/s10393-014-0974-3"},{"key":"Ref43","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.EI10-0004-2015"},{"key":"Ref44","doi-asserted-by":"publisher","DOI":"10.3390\/ani11102865"},{"key":"Ref45","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2017.02.016"},{"key":"Ref46","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-012-1454-0"},{"key":"Ref47","doi-asserted-by":"publisher","DOI":"10.1080\/19768354.2017.1376706"},{"key":"Ref48","unstructured":"Lee J, Park D (2016) The encyclopedia of Korean amphibians. Nature and Ecology Co., Seoul"},{"key":"Ref49","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-012-1316-9"},{"key":"Ref50","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S387215"},{"key":"Ref51","doi-asserted-by":"publisher","DOI":"10.1111\/cobi.14343"},{"key":"Ref52","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1996.0080"},{"key":"Ref53","doi-asserted-by":"crossref","unstructured":"Marschang RE, Meddings JI, Waltzek TB, Hick P, Allender MC, Wirth W, Duffus AL (2024) Ranavirus distribution and host range. In: Gray MJ, Chinchar VG (eds) Ranaviruses: emerging pathogens of ectothermic vertebrates. Springer, Cham, p 155-230","DOI":"10.1007\/978-3-031-64973-8_6"},{"key":"Ref54","doi-asserted-by":"publisher","DOI":"10.3354\/dao02096"},{"key":"Ref55","doi-asserted-by":"publisher","DOI":"10.3390\/v3112351"},{"key":"Ref56","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci12070683"},{"key":"Ref57","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02486-09"},{"key":"Ref58","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-11-46"},{"key":"Ref59","doi-asserted-by":"publisher","DOI":"10.7589\/2015-02-033"},{"key":"Ref60","doi-asserted-by":"publisher","DOI":"10.3354\/dao02443"},{"key":"Ref61","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msu300"},{"key":"Ref62","unstructured":"Park IK, Koo KS, Moon KY, Lee JG, Park D (2017) PCR detection of ranavirus from dead Kaloula borealis and sick Hyla japonica tadpoles in the wild. Korean J Herpetol 8:10-14"},{"key":"Ref63","doi-asserted-by":"publisher","DOI":"10.1186\/s41610-020-00179-2"},{"key":"Ref64","doi-asserted-by":"publisher","DOI":"10.1002\/ecs2.4968"},{"key":"Ref65","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0184768"},{"key":"Ref66","doi-asserted-by":"publisher","DOI":"10.1890\/0012-9658(2006)87[1671:EIDAAP]2.0.CO;2"},{"key":"Ref67","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0157473"},{"key":"Ref68","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0112904"},{"key":"Ref69","doi-asserted-by":"publisher","DOI":"10.5194\/we-24-115-2024"},{"key":"Ref70","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoinf.2014.07.009"},{"key":"Ref71","doi-asserted-by":"publisher","DOI":"10.3390\/v14051073"},{"key":"Ref72","doi-asserted-by":"publisher","DOI":"10.7589\/2018-09-224"},{"key":"Ref73","doi-asserted-by":"publisher","DOI":"10.3390\/v11010026"},{"key":"Ref74","doi-asserted-by":"publisher","DOI":"10.1126\/science.aav0379"},{"key":"Ref75","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocon.2009.02.007"},{"key":"Ref76","doi-asserted-by":"publisher","DOI":"10.3201\/eid1712.110236"},{"key":"Ref77","doi-asserted-by":"publisher","DOI":"10.1002\/ecy.3885"},{"key":"Ref78","doi-asserted-by":"publisher","DOI":"10.1139\/facets-2020-0043"},{"key":"Ref79","doi-asserted-by":"publisher","DOI":"10.3354\/dao02792"},{"key":"Ref80","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2004.02.019"},{"key":"Ref81","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-7-150"},{"key":"Ref82","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2006.04.002"},{"key":"Ref83","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00765-19"},{"key":"Ref84","doi-asserted-by":"crossref","unstructured":"Waltzek TB, Subramaniam K, Jancovich JK (2024) Ranavirus taxonomy and phylogeny In: Gray MJ, Chinchar VG (eds) Ranaviruses: emerging pathogens of ectothermic vertebrates. Springer, Cham, p 7-22","DOI":"10.1007\/978-3-031-64973-8_2"},{"key":"Ref85","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000007588"},{"key":"Ref86","doi-asserted-by":"publisher","DOI":"10.3390\/v10070372"},{"key":"Ref87","unstructured":"WOAH (World Organization for Animal Health) (2019) Manual of diagnostic tests for aquatic animals: Chapter 2.1.2. Infection with ranavirus. www.woah.org\/fileadmin\/Home\/eng\/Health_standards\/aahm\/current\/chapitre_ranavirus.pdf (accessed 18 Dec 2025)"},{"key":"Ref88","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.13534"},{"key":"Ref89","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens12050730"},{"key":"Ref90","doi-asserted-by":"publisher","DOI":"10.1093\/cz\/zoy097"}],"container-title":["Diseases of Aquatic Organisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.int-res.com\/articles\/dao2026\/166\/dao03922.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.int-res.com\/articles\/dao2026\/166\/dao03922.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T11:12:55Z","timestamp":1781781175000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.int-res.com\/abstracts\/dao\/v166\/dao03922"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,18]]},"references-count":90,"URL":"https:\/\/doi.org\/10.3354\/dao03922","archive":["CLOCKSS"],"relation":{},"ISSN":["0177-5103","1616-1580"],"issn-type":[{"value":"0177-5103","type":"print"},{"value":"1616-1580","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,18]]}}}