{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T12:03:49Z","timestamp":1784203429047,"version":"3.55.0"},"reference-count":64,"publisher":"Inter-Research Science Center","license":[{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.int-res.com\/for-librarians\/reader-subscriber-license"}],"content-domain":{"domain":["int-res.com"],"crossmark-restriction":false},"short-container-title":["Dis. Aquat. Org."],"accepted":{"date-parts":[[2026,4,27]]},"abstract":"<jats:p>\n                    <jats:italic>Proteus mirabilis<\/jats:italic>\n                    is a Gram-negative opportunistic pathogen increasingly reported in aquatic animals; however, its pathogenic potential and genomic features in ornamental fish remain unclear. This study isolated 3 strains (WSY-112, WSY-113, and WSY-114) from diseased Asian arowana\n                    <jats:italic>Scleropages formosus<\/jats:italic>\n                    displaying hemorrhagic and ulcerative lesions. All 3 isolates were identified as\n                    <jats:italic>P. mirabilis<\/jats:italic>\n                    through physiological and biochemical characterization as well as 16S rRNA and\n                    <jats:italic>gyrB<\/jats:italic>\n                    sequencing. Strain WSY-114 was selected for further analyses, including whole-genome sequencing, antimicrobial susceptibility testing, and experimental infection. Genomic annotation identified multiple virulence-related genes for flagellar assembly, adhesion, toxin production, iron acquisition, and type\u202fIII\/IV\/VI secretion systems. Experimental infection in zebrafish\n                    <jats:italic>Danio rerio<\/jats:italic>\n                    showed dose-dependent mortality (LD\n                    <jats:sub>50<\/jats:sub>\n                    \u202f=\u202f6.6\u202f\u00d7\u202f10\n                    <jats:sup>4<\/jats:sup>\n                    \u202fCFU g\n                    <jats:sup>-1<\/jats:sup>\n                    ) and clinical signs mirroring natural infections in the arowana. The strain exhibited resistance to multiple tested antibiotics, including tetracyclines (tetracycline, minocycline, and doxycycline), macrolides (erythromycin and azithromycin), and ampicillin, consistent with antibiotic-resistance genes detected in its genome. These results indicate that\n                    <jats:italic>P. mirabilis<\/jats:italic>\n                    exhibits pathogenic potential under experimental conditions and may represent a potential risk factor in ornamental fish.\n                  <\/jats:p>","DOI":"10.3354\/dao03925","type":"journal-article","created":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T11:43:38Z","timestamp":1777895018000},"page":"1-15","update-policy":"https:\/\/doi.org\/10.3354\/policy","source":"Crossref","is-referenced-by-count":0,"title":["Isolation, identification, and genomic features of Proteus mirabilis from diseased Asian arowana Scleropages formosus"],"prefix":"10.3354","volume":"167","author":[{"given":"Han","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyi","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuoran","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8070-5304","authenticated-orcid":true,"given":"Jingfeng","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1913","published-online":{"date-parts":[[2026,7,16]]},"reference":[{"key":"Ref1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003608"},{"key":"Ref2","doi-asserted-by":"publisher","DOI":"10.1128\/ecosalplus.esp-0009-2017"},{"key":"Ref3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000376"},{"key":"Ref4","doi-asserted-by":"publisher","DOI":"10.1038\/75556"},{"key":"Ref5","doi-asserted-by":"publisher","DOI":"10.1111\/eva.12673"},{"key":"Ref6","unstructured":"Behrens AL, Karber L (1953) Determination of LD50. In: Finney DJ (ed) Statistical method in biological assay. Academic Press, New York, NY, p 60-63"},{"key":"Ref7","unstructured":"Bergey DH, Holt JG (1994) Bergey\u2019s manual of determinative bacteriology, 9th edn. Williams & Wilkins, Baltimore, MD"},{"key":"Ref8","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty095"},{"key":"Ref9","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1865504"},{"key":"Ref10","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-8-209"},{"key":"Ref11","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz310"},{"key":"Ref12","doi-asserted-by":"publisher","DOI":"10.1111\/jwas.12936"},{"key":"Ref13","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn663"},{"key":"Ref14","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-9173-0"},{"key":"Ref15","doi-asserted-by":"publisher","DOI":"10.1002\/0471250953.bi0410s05"},{"key":"Ref16","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02742"},{"key":"Ref17","unstructured":"Chen KY, Zuo ZC, Yao CX, Cai DJ (2020) Isolation, identification and drug resistance analysis of Proteus mirabilis from dairy cow. Yunnan Nong Ye Da Xue Xue Bao 35:629-634"},{"key":"Ref18","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki008"},{"key":"Ref19","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-021-00571-5"},{"key":"Ref20","unstructured":"CLSI (Clinical and Laboratory Standards Institute) (2023) Performance standards for antimicrobial susceptibility testing, 33rd edn. CLSI supplement M100. Clinical and Laboratory Standards Institute, Wayne, PA"},{"key":"Ref21","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-015-0720-6"},{"key":"Ref22","doi-asserted-by":"publisher","DOI":"10.1007\/s13213-012-0474-z"},{"key":"Ref23","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02810"},{"key":"Ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2016.10.038"},{"key":"Ref25","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2021.736395"},{"key":"Ref26","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00851-18"},{"key":"Ref27","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-015-0849-0"},{"key":"Ref28","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00019-07"},{"key":"Ref29","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw1004"},{"key":"Ref30","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh063"},{"key":"Ref31","doi-asserted-by":"publisher","DOI":"10.1111\/1749-4877.12510"},{"key":"Ref32","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00321-21"},{"key":"Ref33","doi-asserted-by":"publisher","DOI":"10.1101\/gr.215087.116"},{"key":"Ref34","doi-asserted-by":"publisher","DOI":"10.1101\/gr.092759.109"},{"key":"Ref35","doi-asserted-by":"publisher","DOI":"10.1016\/j.psj.2022.101710"},{"key":"Ref36","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4781(98)00210-3"},{"key":"Ref37","doi-asserted-by":"publisher","DOI":"10.1126\/science.1159483"},{"key":"Ref38","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(00)88945-3"},{"key":"Ref39","doi-asserted-by":"publisher","DOI":"10.1016\/j.mex.2024.103133"},{"key":"Ref40","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt509"},{"key":"Ref41","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.13.4.534"},{"key":"Ref42","doi-asserted-by":"publisher","DOI":"10.2174\/092986711796150469"},{"key":"Ref43","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.06272-11"},{"key":"Ref44","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2018.06.006"},{"key":"Ref45","doi-asserted-by":"publisher","DOI":"10.26028\/cybium\/2004-274-006"},{"key":"Ref46","doi-asserted-by":"publisher","DOI":"10.1039\/B906535K"},{"key":"Ref47","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms12020396"},{"key":"Ref48","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj001"},{"key":"Ref49","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.733668"},{"key":"Ref50","doi-asserted-by":"publisher","DOI":"10.1128\/iai.56.12.3285-3293.1988"},{"key":"Ref51","doi-asserted-by":"publisher","DOI":"10.1016\/S0924-8579(01)00297-7"},{"key":"Ref52","doi-asserted-by":"publisher","DOI":"10.1101\/gr.082081.108"},{"key":"Ref53","doi-asserted-by":"publisher","DOI":"10.1007\/s10142-024-01419-7"},{"key":"Ref54","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2025.06.003"},{"key":"Ref55","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-017-3024-6"},{"key":"Ref56","doi-asserted-by":"publisher","DOI":"10.4103\/ATMPH.ATMPH_511_17"},{"key":"Ref57","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01537-12"},{"key":"Ref58","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M114.044875"},{"key":"Ref59","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj047"},{"key":"Ref60","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-146-10-2385"},{"key":"Ref61","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-017-0844-4"},{"key":"Ref62","doi-asserted-by":"publisher","DOI":"10.1080\/23308249.2019.1697641"},{"key":"Ref63","doi-asserted-by":"publisher","DOI":"10.1016\/j.aaf.2021.05.007"},{"key":"Ref64","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2024.1381832"}],"container-title":["Diseases of Aquatic Organisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.int-res.com\/articles\/dao2026\/167\/dao03925.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.int-res.com\/articles\/dao2026\/167\/dao03925.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T11:11:28Z","timestamp":1784200288000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.int-res.com\/abstracts\/dao\/v167\/dao03925"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,16]]},"references-count":64,"URL":"https:\/\/doi.org\/10.3354\/dao03925","archive":["CLOCKSS"],"relation":{},"ISSN":["0177-5103","1616-1580"],"issn-type":[{"value":"0177-5103","type":"print"},{"value":"1616-1580","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,16]]}}}