{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T12:41:40Z","timestamp":1792672900041,"version":"4.5.0"},"reference-count":46,"publisher":"Inter-Research Science Center","license":[{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.int-res.com\/for-librarians\/reader-subscriber-license"}],"content-domain":{"domain":["int-res.com"],"crossmark-restriction":false},"short-container-title":["Dis. Aquat. Org."],"accepted":{"date-parts":[[2026,7,16]]},"abstract":"<jats:p>\n                    Tilapia (genus\n                    <jats:italic>Oreochromis<\/jats:italic>\n                    ) contribute significantly to food security and aquaculture development in the Department of San Mart\u00edn, an administrative region in the Peruvian Amazon. During the final quarter of 2024, an investigation was carried out following reports of infectious disease outbreaks associated with unusually high mortality in farmed tilapia located in the provinces of Mariscal C\u00e1ceres and Bellavista. A total of 153 samples were analysed, including 120 broodstock, 24 fry, and 9 juvenile specimens. PCR testing detected\n                    <jats:italic>Streptococcus agalactiae<\/jats:italic>\n                    in at least 1 juvenile sample from each of Farms A and B, representing the first confirmed cases of\n                    <jats:italic>S. agalactiae<\/jats:italic>\n                    associated with the outbreaks. In the hatchery, microbiological analysis of broodstock and fry samples yielded 6 bacterial isolates compatible with\n                    <jats:italic>Streptococcus<\/jats:italic>\n                    spp., which were subsequently confirmed as\n                    <jats:italic>S. agalactiae<\/jats:italic>\n                    by PCR. At Farm C,\n                    <jats:italic>S. agalactiae<\/jats:italic>\n                    was confirmed through PCR, and 3 bacterial isolates (P01, P02, and P03) were recovered and comprehensively characterized. Molecular analyses confirmed all isolates as\n                    <jats:italic>S. agalactiae<\/jats:italic>\n                    . Further characterization identified serotype Ia using conventional PCR and sequence type ST7 through multilocus sequence typing (MLST). Three representative isolates obtained from diseased juvenile tilapia were further characterized by whole-genome sequencing and MLST analysis, confirming their classification as\n                    <jats:italic>S. agalactiae<\/jats:italic>\n                    serotype Ia sequence type ST7. Clinical signs observed during the outbreaks included erratic swimming, lethargy, exophthalmos, fin erosion, and hepatic alterations, consistent with streptococcosis. This study represents the first molecular characterization of\n                    <jats:italic>S. agalactiae<\/jats:italic>\n                    serotype Ia ST7 associated with streptococcosis outbreaks in farmed tilapia in Peru.\n                  <\/jats:p>","DOI":"10.3354\/dao03938","type":"journal-article","created":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T15:23:56Z","timestamp":1786116236000},"page":"1-12","update-policy":"https:\/\/doi.org\/10.3354\/policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular identification and characterization of Streptococcus agalactiae serotype Ia (ST7) associated with streptococcosis outbreaks in farmed tilapia in San Mart\u00edn, Peru"],"prefix":"10.3354","volume":"168","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1006-182X","authenticated-orcid":true,"given":"Anghela Vanessa","family":"Mogoll\u00f3n-Calder\u00f3n","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7700-0848","authenticated-orcid":true,"given":"Francisco Miroslav","family":"Ulloa-Stanojlovic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4392-9026","authenticated-orcid":true,"given":"Stephanie","family":"Tapia-Chirinos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Armando","family":"Yong-Rojas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine","family":"Zapata Lovera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose","family":"Rodr\u00edguez-Callan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodolfo","family":"Velazco-Pe\u00f1a","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7335-2902","authenticated-orcid":true,"given":"Marco Cesar","family":"Medina Morillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Quevedo-Alvarado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5406-4443","authenticated-orcid":true,"given":"Muriel","family":"G\u00f3mez-S\u00e1nchez Orezzoli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1913","published-online":{"date-parts":[[2026,10,22]]},"reference":[{"key":"Ref1","doi-asserted-by":"publisher","DOI":"10.3390\/biology13110914"},{"key":"Ref2","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-024-04298-z"},{"key":"Ref3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0007421"},{"key":"Ref4","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-13228-z"},{"key":"Ref5","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.00121"},{"key":"Ref6","doi-asserted-by":"publisher","DOI":"10.21105\/joss.05968"},{"key":"Ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.aaf.2024.05.002"},{"key":"Ref8","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty149"},{"key":"Ref9","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-13-41"},{"key":"Ref10","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12319"},{"key":"Ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2021.105084"},{"key":"Ref12","unstructured":"FAO (2021) Risk profile - Group B Streptococcus (GBS) - Streptococcus agalactiae sequence type (ST) 283 in freshwater fish. 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