{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T20:04:36Z","timestamp":1762113876523},"reference-count":0,"publisher":"Institute of Physiology of the Czech Academy of Sciences","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Physiol Res"],"published-print":{"date-parts":[[2008]]},"abstract":"<jats:p>Dendritic cell (DC) vaccination is an attractive approach to the\ntreatment of patients with lymphoid tumors. To evaluate its\nfeasibility, we have tested the functional properties of DC and\nT-lymphocytes in patients with treated and untreated chronic\nlymphocytic leukemia (CLL) and follicular lymphoma (FL). Healthy\nvolunteers were used both as controls and as a source of cells for\nallogeneic mixed leukocyte reaction (MLR). In these reactions,\ndendritic cells from both untreated and treated patients were\ncomparable to dendritic cells from healthy volunteers. In all the\nuntreated patients studied, autologous dendritic cells promoted\nthe survival and proliferation of both CD4 and CD8 lymphocytes\n(though the proliferation response was much better in the CD4\nsubset), whereas only 3 out of 5 treated patients were able to\nmount this response with CD4 lymphocytes and 4 out of 5 with\nCD8 lymphocytes. In 3 out of 5 untreated patients, pulsing of\nDCs with tetanus toxoid promoted a better CD4 response than\nwas achieved with unpulsed DCs, while none of 5 treated\npatients had an additional response after pulsing with tetanus\ntoxoid. None of patients studied, either treated or untreated, had\na better CD8 response to pulsed DCs than to unpulsed ones.\nDuring CD4 lymphocyte proliferation, more CD4+CD25hi\nlymphocytes were generated in both treated and untreated\npatients than in healthy controls. Poor proliferation of cytotoxic\ncells and preferential proliferation of CD4+CD25hi T-regulatory\ncells in response to self and\/or foreign antigens might be one of\nthe mechanisms responsible for immunosuppression and\nimpaired tumor surveillance in patients with lymphoid\nmalignancies.<\/jats:p>","DOI":"10.33549\/physiolres.930857","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T16:58:16Z","timestamp":1619456296000},"page":"289-298","source":"Crossref","is-referenced-by-count":1,"title":["Dendritic cells and T lymphocyte interactions in patients with lymphoid malignancies"],"prefix":"10.33549","author":[{"given":"R","family":"Pytl\u00edk","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Hofman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Kideryov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"\u010cervinkov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Obrtl\u00edkov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"\u0160\u00e1lkov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Trn\u011bn\u00fd","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Klener","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"19169","published-online":{"date-parts":[[2008]]},"container-title":["Physiological Research"],"original-title":[],"language":"en","deposited":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T16:58:17Z","timestamp":1619456297000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.biomed.cas.cz\/physiolres\/pdf\/57\/57_289.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008]]},"references-count":0,"URL":"https:\/\/doi.org\/10.33549\/physiolres.930857","relation":{},"ISSN":["1802-9973","0862-8408"],"issn-type":[{"value":"1802-9973","type":"electronic"},{"value":"0862-8408","type":"print"}],"subject":[],"published":{"date-parts":[[2008]]}}}