{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T17:55:59Z","timestamp":1762624559727},"reference-count":0,"publisher":"Institute of Physiology of the Czech Academy of Sciences","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Physiol Res"],"published-print":{"date-parts":[[2009]]},"abstract":"<jats:p>The oxidative mechanisms of injury-induced damage of neurons\nwithin the spinal cord are not very well understood. We used a\nmodel of T8-T9 spinal cord injury (SCI) in the rat to induce\nneuronal degeneration. In this spinal cord injury model, unilateral\navulsion of the spinal cord causes oxidative stress of neurons. We\ntested the hypothesis that apurinic\/apyrimidinic endonuclease (or\nredox effector factor-1, APE\/Ref-1) regulates this neuronal\noxidation mechanism in the spinal cord region caudal to the\nlesion, and that DNA damage is an early upstream signal. The\nembryonic neural stem cell therapy significantly decreased DNAdamage levels in both study groups \u2013 acutely (followed up to\n7 days after SCI), and chronically (followed up to 28 days after\nSCI) injured animals. Meanwhile, mRNA levels of APE\/Ref-1\nsignificantly increased after embryonic neural stem cell therapy in\nacutely and chronically injured animals when compared to acute\nand chronic sham groups. Our data has demonstrated that an\nincrease of APE\/Ref-1 mRNA levels in the caudal region of spinal\ncord strongly correlated with DNA damage after traumatic spinal\ncord injury. We suggest that DNA damage can be observed both\nin lesional and caudal regions of the acutely and chronically\ninjured groups, but DNA damage is reduced with embryonic\nneural stem cell therapy.<\/jats:p>","DOI":"10.33549\/physiolres.931388","type":"journal-article","created":{"date-parts":[[2021,2,18]],"date-time":"2021-02-18T09:01:03Z","timestamp":1613638863000},"page":"427-434","source":"Crossref","is-referenced-by-count":10,"title":["Alterations in the expression of the apurinic\/apyrimidinic endonuclease-1\/redox factor-1 (APE\/ref-1) and DNA damage in the caudal region of acute and chronic spinal cord injured rats treated by embryonic neural stem cells"],"prefix":"10.33549","author":[{"given":"T","family":"Dagci","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Armagan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Konyalioglu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Yalcin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"19169","published-online":{"date-parts":[[2009]]},"container-title":["Physiological Research"],"original-title":[],"language":"en","deposited":{"date-parts":[[2021,2,18]],"date-time":"2021-02-18T09:01:04Z","timestamp":1613638864000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.biomed.cas.cz\/physiolres\/pdf\/58\/58_427.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009]]},"references-count":0,"URL":"https:\/\/doi.org\/10.33549\/physiolres.931388","relation":{},"ISSN":["1802-9973","0862-8408"],"issn-type":[{"value":"1802-9973","type":"electronic"},{"value":"0862-8408","type":"print"}],"subject":[],"published":{"date-parts":[[2009]]}}}