{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T10:30:43Z","timestamp":1758709843417},"reference-count":0,"publisher":"Institute of Physiology of the Czech Academy of Sciences","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Physiol Res"],"published-print":{"date-parts":[[2010]]},"abstract":"<jats:p>The present study investigated cardiac function in hearts of mice\nwith total deficiency of the \u03b21-, \u03b22- and \u03b23-adrenoceptors (TKO) in\ncomparison to wildtype mice (WT). We investigated cardiac\nmorphology and echocardiographic function, measured protein\nexpression of Ca2+-regulatory proteins, SERCA 2a activity,\nmyofibrillar function, and performed running wheel tests. Heart\nweight and heart-to-body weight ratio were significantly smaller\nin TKO as compared to WT. This was accompanied by a decrease\nin the size of the cardiomyocytes in TKO. Heart rate and ejection\nfraction were significantly diminished in TKO as compared to WT.\nProtein expressions of SERCA 2a, ryanodine receptor and\nNa+\/Ca2+-exchanger were similar in TKO and WT mice, but\nphospholamban protein expression was increased. PKAdependent phosphorylation of phospholamban at serine 16 was absent and CaMKII-dependent phosphorylation at threonine 17 was decreased in TKO. All alterations were paralleled by a decrease in SERCA 2a-activity. A similar maximal calciumdependent tension but an increased myofibrillar calciumsensitivity was measured in TKO as compared to WT. We did not observe relevant functional impairments of TKO in running wheel\ntests. In the absence of \u03b2-agonistic stimulation, SERCA 2a activity\nis mainly regulated by alterations of phospholamban expression\nand phosphorylation. The decreased SERCA 2a activity following\n\u03b2-adrenoceptor deficiency may be partly compensated by an\nincreased myofibrillar calcium-sensitivity.<\/jats:p>","DOI":"10.33549\/physiolres.931851","type":"journal-article","created":{"date-parts":[[2021,2,2]],"date-time":"2021-02-02T11:49:18Z","timestamp":1612266558000},"page":"679-689","source":"Crossref","is-referenced-by-count":4,"title":["Total \u03b2-adrenoceptor deficiency results in cardiac hypotrophy and negative inotropy"],"prefix":"10.33549","author":[{"given":"S","family":"Lee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Grafweg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Schneider","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Jimenez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J-P","family":"Giacobino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Ghanem","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Tiemann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W","family":"Bloch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"M\u00fcller-Ehmsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"RHG","family":"Schwinger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Brixius","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"19169","published-online":{"date-parts":[[2010]]},"container-title":["Physiological Research"],"original-title":[],"language":"en","deposited":{"date-parts":[[2021,2,3]],"date-time":"2021-02-03T16:10:53Z","timestamp":1612368653000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.biomed.cas.cz\/physiolres\/pdf\/59\/59_679.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010]]},"references-count":0,"URL":"https:\/\/doi.org\/10.33549\/physiolres.931851","relation":{},"ISSN":["1802-9973","0862-8408"],"issn-type":[{"value":"1802-9973","type":"electronic"},{"value":"0862-8408","type":"print"}],"subject":[],"published":{"date-parts":[[2010]]}}}