{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T18:27:05Z","timestamp":1791224825209,"version":"4.1.0"},"reference-count":0,"publisher":"Institute of Physiology of the Czech Academy of Sciences","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Physiol Res"],"published-print":{"date-parts":[[2009]]},"abstract":"<jats:p>GABA exhibits depolarizing action in the immature neurons due\nto high intracellular activity of chloride ions. It is maintained by\ncation-chloride cotransporter NKCC1 which is present in\nimmature brain. Bumetanide is a specific inhibitor of this\ncotransporter. We studied possible anticonvulsant activity of\nbumetanide in pentylenetetrazol-induced seizures in three age\ngroups of rat pups (7, 12, and 18 days old). Pretreatment with\nbumetanide (0.2-1 mg\/kg i.p.) resulted in dose-dependent\ndecrease of incidence of the tonic phase of generalized tonicclonic seizures in 12-day-old rats only. No effect was observed in\nyounger and older animals. Higher dose of bumetanide\n(2.5 mg\/kg) did not affect tonic convulsions but, on the contrary,\ndecreased latencies of generalized seizures in 12-day-old\nanimals. Lack of marked anticonvulsant effect is probably due to\nrelative maturity of neurons in the brainstem where the\ngenerator of generalized seizures is localized. Age- and dosespecific suppression of the tonic phase needs further analysis.<\/jats:p>","DOI":"10.33549\/physiolres.931897","type":"journal-article","created":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T16:23:58Z","timestamp":1614615838000},"page":"927-930","source":"Crossref","is-referenced-by-count":24,"title":["Age- and dose-specific anticonvulsant action of bumetanide in immature rats"],"prefix":"10.33549","author":[{"given":"P","family":"Mare\u0161","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"19169","published-online":{"date-parts":[[2009]]},"container-title":["Physiological Research"],"original-title":[],"language":"en","deposited":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T16:23:59Z","timestamp":1614615839000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.biomed.cas.cz\/physiolres\/pdf\/58\/58_927.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009]]},"references-count":0,"URL":"https:\/\/doi.org\/10.33549\/physiolres.931897","relation":{},"ISSN":["1802-9973","0862-8408"],"issn-type":[{"value":"1802-9973","type":"electronic"},{"value":"0862-8408","type":"print"}],"subject":[],"published":{"date-parts":[[2009]]}}}