{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T22:41:38Z","timestamp":1781649698784,"version":"3.54.5"},"reference-count":27,"publisher":"Institute of Physiology of the Czech Academy of Sciences","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Physiol Res"],"published-print":{"date-parts":[[2012,6,30]]},"abstract":"<jats:p>UVB radiation from sunlight induces an acute corneal inflammation, photokeratitis, accompanied by changes in corneal hydration. We employed a method of ultrasonic pachymetry for daily examination of central corneal thickness as an index of corneal hydration of the rabbit cornea repeatedly irradiated by UVB radiation (312 nm, daily dose of 0.25 J\/cm2 during three or four days) as influenced by UVB absorber (actinoquinol combined with hyaluronic acid) dropped on the ocular surface during irradiation. One day after the third irradiation procedure the animals were sacrificed and corneas examined immuno-histochemically for peroxynitrite formation, a marker of oxidative damage, the antioxidant aldehyde dehydrogenase 3A1 and endothelial nitric oxide synthase, an enzyme generated nitric oxide. Results show that UV absorber combined with hyaluronic acid protected the cornea against UVB-induced changes in corneal thickness and microscopical disturbances to the cornea (both seen after buffered saline application) until the fourth experimental day. These UVB doses are equivalent to a daily exposure of 2.5 hrs of the human cornea to solar UVB radiation for three consecutive days. It is suggested that actinoquinol\/ hyaluronic acid drops might be helpful for the human eye in the defence against photooxidative and other oxidative processes.<\/jats:p>","DOI":"10.33549\/physiolres.932242","type":"journal-article","created":{"date-parts":[[2020,4,5]],"date-time":"2020-04-05T10:03:58Z","timestamp":1586081038000},"page":"299-306","source":"Crossref","is-referenced-by-count":15,"title":["Central Corneal Thickness Considered an Index of Corneal Hydration of the UVB Irradiated Rabbit Cornea as Influenced by UVB Absorber"],"prefix":"10.33549","author":[{"given":"\u010c.","family":"\u010cEJKA","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"LUYCKX","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"\u010cEJKOV\u00c1","sequence":"additional","affiliation":[{"name":"Laboratory of Eye Histochemistry and Pharmacology, Institute of Experimental Medicine, Center of Excellence, Academy of Sciences of the Czech Republic, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"19169","published-online":{"date-parts":[[2012,6,16]]},"reference":[{"key":"ref0","doi-asserted-by":"publisher","unstructured":"\u010cEJKA \u010c, PL\u00c1TEN\u00cdK J, GURYCA V, MICH\u00c1LEK J, BR\u016eNOV\u00c1 B, \u010cEJKOV\u00c1 J: Light absorption properties of the rabbit cornea repeatedly irradiated with UVB rays. Photochem Photobiol 83: 652-657, 2007.","DOI":"10.1111\/j.1751-1097.2007.00061.x"},{"key":"ref1","doi-asserted-by":"publisher","unstructured":"\u010cEJKA \u010c, LUYCKX J, ARDAN T, \u010cEJKOV\u00c1 J: The effect of actinoquinol with hyaluronic acid in eye drops on the optical properties and oxidative damage of the rabbit cornea irradiated with UVB rays. Photochem Photobiol 86: 1294-1306, 2010.","DOI":"10.1111\/j.1751-1097.2010.00796.x"},{"key":"ref2","doi-asserted-by":"publisher","unstructured":"\u010cEJKA \u010c, ARDAN T, \u0160IRC J, MICH\u00c1LEK J, BENE\u0160 J, BR\u016eNOV\u00c1 B, ROSINA J: Hydration and transparency of the rabbit cornea irradiated with UVB-doses of 0.25 J\/cm2 and 0.5 J\/cm2 compared with equivalent UVB radiation exposure reaching the human cornea from sunlight. 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Mol Vision 15: 577-583, 2009."},{"key":"ref17","unstructured":"ROSENTHAL FS, PHOON C, BAKALIAN AE, TAYLOR HR: The ocular dose of ultraviolet radiation to outdoor workers. Invest Ophthalmol Vis Sci 29: 649-656, 1988."},{"key":"ref18","unstructured":"SASAKI H: Sun\u00b4s UV rays more dangerous in snow than on the beach. (Authorized interview given by Hiroshi Sasaki to Danielle Demetriou, Tokyo, for The Daily Telegraph), 2009. Available from: http:\/\/www.telegraph.co.uk\/science\/6766419\/Suns-UV-rays."},{"key":"ref19","doi-asserted-by":"publisher","unstructured":"SASAKI H, KAWAKAMI Y, ONO M, JONASSON F, SHUI YB, CHENG HM, ROBMAN L, MCCARTY C, CHEW SJ, SASAKI K: Localization of cortical cataract in subjects of diverse races and latitude. Invest Ophthalmol Vis Sci 44: 4210-4214, 2003.","DOI":"10.1167\/iovs.01-1221"},{"key":"ref20","doi-asserted-by":"crossref","unstructured":"SLINEY DH: UV radiation ocular exposure dosimetry. 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