{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T05:49:10Z","timestamp":1771307350573,"version":"3.50.1"},"reference-count":39,"publisher":"Institute of Physiology of the Czech Academy of Sciences","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Physiol Res"],"published-print":{"date-parts":[[2013,10,31]]},"abstract":"<jats:p>Hypolipidemic compound pirinixic acid (WY-14643, WY) is known to exert pleiotropic (other than primary) effects, such as activation of peroxisome proliferator-activated receptors (PPAR-\u03b1), transcription factors regulating different cardiac functions. Their role in ischemia-reperfusion (I\/R) injury and cardioprotection is less clear, although protective effects of PPAR agonists have been documented. This study was designed to explore the effects of WY on the I\/R injury in the rat heart and potential mechanisms involved, including mitochondrial KATP channels (mitoKATP) opening and production of reactive oxygen species (ROS). Langendorff-perfused hearts of rats intragastrally treated with WY (3 mg\/kg\/day) for 5 days and of control animals were subjected to 30-min global ischemia and 2-h reperfusion with or without 15-min perfusion with mitoKATP blocker 5-hydroxydecanoate (5-HD) prior to I\/R. Evaluation of the infarct size (IS, TTC staining) served as the main end-point of protection. Lipid peroxidation (a marker of ROS production) was determined by measurement of myocardial concentration of conjugated dienes (CD), whereas protein expression of endothelial NO synthase was analysed by Western blotting. A 2-fold increase in the cardiac protein levels of eNOS after treatment with WY was accompanied by lower post-I\/R levels of CD compared with those in the hearts of untreated controls, although WY itself enhanced ROS generation prior to ischemia. IS was reduced by 47 % in the hearts of WY-treated rats (P&lt;0.05), and this effect was reversed by 5-HD. Results suggest that PPAR-\u03b1 activation may confer protection against lethal I\/R injury in the rat heart that involves up-regulation of eNOS, mitoKATP opening and reduced oxidative stress during I\/R.<\/jats:p>","DOI":"10.33549\/physiolres.932591","type":"journal-article","created":{"date-parts":[[2020,8,6]],"date-time":"2020-08-06T08:30:20Z","timestamp":1596702620000},"page":"577-584","source":"Crossref","is-referenced-by-count":6,"title":["Treatment of Rats With Hypolipidemic Compound Pirinixic Acid Protects Their Hearts Against Ischemic Injury: Are Mitochondrial KATP Channels and Reactive Oxygen Species Involved?"],"prefix":"10.33549","author":[{"given":"M.","family":"NEM\u010cEKOV\u00c1","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"\u010cARNICK\u00c1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"FERKO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"MUR\u00c1RIKOV\u00c1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"LEDV\u00c9NYIOV\u00c1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"RAVINGEROV\u00c1","sequence":"additional","affiliation":[{"name":"Institute for Heart Research, Slovak Academy of Sciences, Centre of Excellence of SAS NOREG, Bratislava, Slovak Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"19169","published-online":{"date-parts":[[2013,10,17]]},"reference":[{"key":"ref0","unstructured":"ANDELOV\u00c1 E, BARTEKOV\u00c1 M, PANCZA D, STYK J, RAVINGEROV\u00c1 T: The role of NO in ischemia\/reperfusion injury in isolated rat heart. 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