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The aim was to find out whether separate effects of RPC and DM would stimulate the mechanisms of cardioprotection to a maximal level or whether RPC and DM conditions would cooperate in stimulation of cardioprotection. Experiments were performed on male Wistar rats divided into groups: control, DM, RPC and DM treated by RPC (RPC+DM). RPC protocol of 3 cycles of 5-min hind limb ischemia followed by 5-min reperfusion was used. Ischemic-reperfusion injury was induced by 30-min ischemia followed by 40-min reperfusion of the hearts in Langendorff mode. Mitochondria were isolated by differential centrifugation, infarct size assessed by staining with 1 % 2,3,5-triphenyltetrazolium chloride, mitochondrial membrane fluidity with a fluorescent probe DPH, CoQ9 and CoQ10 with HPLC. Results revealed that RPC as well as DM decreased the infarct size and preserved mitochondrial function by increasing the mitochondrial membrane fluidity. Both used models separately offered a sufficient protection against ischemic-reperfusion injury without an additive effect of their combination.<\/jats:p>","DOI":"10.33549\/physiolres.933218","type":"journal-article","created":{"date-parts":[[2020,4,15]],"date-time":"2020-04-15T08:25:34Z","timestamp":1586939134000},"page":"S617-S625","source":"Crossref","is-referenced-by-count":6,"title":["Participation of Heart Mitochondria in Myocardial Protection Against Ischemia\/Reperfusion Injury: Benefit Effects of Short-Term Adaptation Processes"],"prefix":"10.33549","author":[{"given":"M.","family":"FERKO","sequence":"first","affiliation":[{"name":"Institute for Heart Research, Slovak Academy of Sciences, Centre of Excellence of SAS NOREG, Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"KANCIROV\u00c1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"JA\u0160OV\u00c1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"WACZUL\u00cdKOV\u00c1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"\u010cARNICK\u00c1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"KUCHARSK\u00c1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"ULI\u010cN\u00c1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"VAN\u010cOV\u00c1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"MUR\u00c1RIKOV\u00c1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"RAVINGEROV\u00c1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"ZIEGELH\u00d6FFER","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"19169","published-online":{"date-parts":[[2015,12,14]]},"reference":[{"key":"ref0","doi-asserted-by":"publisher","unstructured":"BAKKER A, DE BIE M, BERNAERT I, RAVINGEROVA T, ZIEGELH\u00d6FFER A, VAN BELLE H, JACOB W: Increased extracellular calcium concentrations in the isolated rat heart: effects on mitochondrial contact site formation. 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