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However, the change of expression of CB1-Rs and CB2-Rs involve in 2Hz EA anti-relapse pathway is still unclear. To explore the changes of expression of CB1-Rs and CB2-Rs, heroin self-administration (SA) model rats were adopted and treated using 2Hz EA. The expressions of CB1-Rs and CB2-Rs were observed using immunohistochemistry method. The results showed that, compared with the control group, active pokes in the heroin-addicted group increased, while the active pokes decreased significantly in 2Hz EA group compared with heroin-addicted group. Correspondingly, the expression of CB1-Rs in prefrontal cortex (PFC), hippocampus (Hip), nucleus accumbens (NAc) and ventral tegmental area (VTA) all increased significantly while the expression of CB2-Rs in those relapse-relevant brain regions decreased obviously in heroin-addicted group when compared with the control group. In addition, the expression of CB1-Rs obviously decreased in the 2Hz EA group while the expression of CB2-Rs in those relapse-relevant brain regions increased significantly when compared with the heroin-addicted group. It indicated that 2Hz EA could attenuate the heroin-evoked seeking behaviors effectively. The anti-relapse effects of 2Hz EA might be related to the decrease of CB1-Rs and increase of CB2-Rs expression in relapse-relevant brain regions of heroin SA rats.<\/jats:p>","DOI":"10.33549\/physiolres.934106","type":"journal-article","created":{"date-parts":[[2019,11,13]],"date-time":"2019-11-13T07:16:31Z","timestamp":1573629391000},"page":"835-844","source":"Crossref","is-referenced-by-count":4,"title":["2Hz-Electroacupuncture Attenuates Heroin-Seeking Behaviors via Adjusts CB1-Rs and CB2-Rs Expression in Relapse-Relevant Brain Regions of Heroin Self-Administration Rats"],"prefix":"10.33549","author":[{"given":"L.","family":"CHEN","sequence":"first","affiliation":[{"name":"School of Physical Education, Jianghan University, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X.-K.","family":"GONG","sequence":"additional","affiliation":[{"name":"Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.-L.","family":"LENG","sequence":"additional","affiliation":[{"name":"Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.-M.","family":"MA","sequence":"additional","affiliation":[{"name":"Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Q.","family":"RU","sequence":"additional","affiliation":[{"name":"Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Q.","family":"XIONG","sequence":"additional","affiliation":[{"name":"Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"YUE","sequence":"additional","affiliation":[{"name":"Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"ZHOU","sequence":"additional","affiliation":[{"name":"Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X.","family":"TIAN","sequence":"additional","affiliation":[{"name":"Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.-Y.","family":"LI","sequence":"additional","affiliation":[{"name":"Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.-X.","family":"WU","sequence":"additional","affiliation":[{"name":"School of Physical Education, Jianghan University, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"19169","published-online":{"date-parts":[[2019,10,31]]},"reference":[{"key":"ref0","doi-asserted-by":"publisher","unstructured":"BAYEWITCH M, AVIDOR-REISS T, LEVY R, BARG J, MECHOULAM R, VOGEL Z: The peripheral cannabinoid receptor: adenylate cyclase inhibition and G protein coupling. 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